⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 13-DEC-2020

⊟Summary

• organism: Streptococcus pneumoniae SPN994039
locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
pan locus tag^?: PNEUPAN003613000
symbol: SPN994039_RS09850
pan gene symbol^?: —
• synonym:
• product: class I SAM-dependent methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
• symbol: SPN994039_RS09850
• product: class I SAM-dependent methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1820103..1821056
• length: 954
• essential: unknown

⊟Accession numbers

• Location: NC_021005 (1820103..1821056) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1854 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGATTTTGAAAAAATTGAACAAGCTTATACCTATTTACTAGAGAATGTCCAAGTCATC
  CAAAGTGATTTGGCGACCAACTTTTATGACGCCTTGGTGGAGCAAAACAGCATCTATCTG
  GATGGTGAGACTGAGCTAGAACAGGTCAAAGACAACAATCAGACCCTTAAGCGTTTAGCA
  CTACGCAAAGAAGAATGGCTCAAGACCTACCAGTTTCTCTTGATGAAGGCTGGGCAAACA
  GAACCCTTGCAGGCCAATCACCAGTTTACACCGGATGCTATTGCTTTACTTTTGGTGTTT
  ATTGTGGAAGAGCTGTTTAAAGAGGAGGAAATTACTATCCTCGAAATGGGTTCTGGGATG
  GGAATTTTGGGCGCTACTTTCTTGACCTCGCTTACTAAAAAGGTGGATTATTTGGGAATG
  GAAGTGGATGATTTGCTGATTGATCTGGCAGCTAGCATGGCAGATGTAATTGGTTTGCAG
  GCTGGCTTTGTCCAAGGAGATGCCGTTCGTCCACAAATGCTCAAAGAAAGCGATGTGGTC
  ATCAGTGACTTGCCTGTTGGCTATTATCCTGATGATGCCGTTGCGTCGCGCCATCAAGTT
  GCTTCTAGCCAAGAACATACTTACGCCCATCACTTGCTCATGGAACAAGGGCTTAAGTAC
  CTCAAGTCAGACGGATACGCTATTTTTCTAGCTCCGAGTGATTTGTTGACCAGTCCTCAA
  AGTGATTTGTTAAAAGAATGGCTGAAAGAAGAGGCGAGTCTGGTTGCTATGATTAGTCTG
  CCTGAAAATCTCTTTGCTAATGCTAAACAATCTAAGACTATTTTTATCCTACAGAAGAAA
  AGTGAAATAGCAGTGGAGCCTTTTGTTTATCCACTTGCTAGCTTGCAAGATGCAAGTGTT
  TTAATGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  954
  

⊟Protein

⊟General

• locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
• symbol: SPN994039_RS09850
• description: class I SAM-dependent methyltransferase
• length: 317
• theoretical pI: 4.24752
• theoretical MW: 35718.6
• GRAVY: -0.066877

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 20.7)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 20.5)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 17.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 17.3)
  and 3 more
  Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 15.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 15.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.8)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 37.5)
  and 5 more
  MTS; Methyltransferase small domain (PF05175; HMM-score: 19.5)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 19.3)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 18.5)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.1)
  Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002563
  • TAT(Tat/SPI): 0.00015
  • LIPO(Sec/SPII): 0.000428
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000345099 NCBI
• UniProt:

⊟Protein sequence

• MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGETELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIVEELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQAGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGLKYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFILQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1854 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications