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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
  • symbol: SPN994039_RS09850
  • product: class I SAM-dependent methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1820103..1821056
  • length: 954
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_021005 (1820103..1821056) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1854 PneumoBrowse

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTTTGAAAAAATTGAACAAGCTTATACCTATTTACTAGAGAATGTCCAAGTCATC
    CAAAGTGATTTGGCGACCAACTTTTATGACGCCTTGGTGGAGCAAAACAGCATCTATCTG
    GATGGTGAGACTGAGCTAGAACAGGTCAAAGACAACAATCAGACCCTTAAGCGTTTAGCA
    CTACGCAAAGAAGAATGGCTCAAGACCTACCAGTTTCTCTTGATGAAGGCTGGGCAAACA
    GAACCCTTGCAGGCCAATCACCAGTTTACACCGGATGCTATTGCTTTACTTTTGGTGTTT
    ATTGTGGAAGAGCTGTTTAAAGAGGAGGAAATTACTATCCTCGAAATGGGTTCTGGGATG
    GGAATTTTGGGCGCTACTTTCTTGACCTCGCTTACTAAAAAGGTGGATTATTTGGGAATG
    GAAGTGGATGATTTGCTGATTGATCTGGCAGCTAGCATGGCAGATGTAATTGGTTTGCAG
    GCTGGCTTTGTCCAAGGAGATGCCGTTCGTCCACAAATGCTCAAAGAAAGCGATGTGGTC
    ATCAGTGACTTGCCTGTTGGCTATTATCCTGATGATGCCGTTGCGTCGCGCCATCAAGTT
    GCTTCTAGCCAAGAACATACTTACGCCCATCACTTGCTCATGGAACAAGGGCTTAAGTAC
    CTCAAGTCAGACGGATACGCTATTTTTCTAGCTCCGAGTGATTTGTTGACCAGTCCTCAA
    AGTGATTTGTTAAAAGAATGGCTGAAAGAAGAGGCGAGTCTGGTTGCTATGATTAGTCTG
    CCTGAAAATCTCTTTGCTAATGCTAAACAATCTAAGACTATTTTTATCCTACAGAAGAAA
    AGTGAAATAGCAGTGGAGCCTTTTGTTTATCCACTTGCTAGCTTGCAAGATGCAAGTGTT
    TTAATGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
  • symbol: SPN994039_RS09850
  • description: class I SAM-dependent methyltransferase
  • length: 317
  • theoretical pI: 4.24752
  • theoretical MW: 35718.6
  • GRAVY: -0.066877

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 20.7)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 20.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 17.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 17.3)
    and 3 more
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 15.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.8)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 37.5)
    and 5 more
    MTS; Methyltransferase small domain (PF05175; HMM-score: 19.5)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 19.3)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 18.5)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.1)
    Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002563
    • TAT(Tat/SPI): 0.00015
    • LIPO(Sec/SPII): 0.000428
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000345099 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGETELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIVEELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQAGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGLKYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFILQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI

Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Expression data[edit | edit source]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

NCBI: 13-DEC-2020

Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN994039
  • locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
  • pan locus tag?: PNEUPAN003613000
  • symbol: SPN994039_RS09850
  • pan gene symbol?:
  • synonym:
  • product: class I SAM-dependent methyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
  • symbol: SPN994039_RS09850
  • product: class I SAM-dependent methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1820103..1821056
  • length: 954
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_021005 (1820103..1821056) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1854 PneumoBrowse

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTTTGAAAAAATTGAACAAGCTTATACCTATTTACTAGAGAATGTCCAAGTCATC
    CAAAGTGATTTGGCGACCAACTTTTATGACGCCTTGGTGGAGCAAAACAGCATCTATCTG
    GATGGTGAGACTGAGCTAGAACAGGTCAAAGACAACAATCAGACCCTTAAGCGTTTAGCA
    CTACGCAAAGAAGAATGGCTCAAGACCTACCAGTTTCTCTTGATGAAGGCTGGGCAAACA
    GAACCCTTGCAGGCCAATCACCAGTTTACACCGGATGCTATTGCTTTACTTTTGGTGTTT
    ATTGTGGAAGAGCTGTTTAAAGAGGAGGAAATTACTATCCTCGAAATGGGTTCTGGGATG
    GGAATTTTGGGCGCTACTTTCTTGACCTCGCTTACTAAAAAGGTGGATTATTTGGGAATG
    GAAGTGGATGATTTGCTGATTGATCTGGCAGCTAGCATGGCAGATGTAATTGGTTTGCAG
    GCTGGCTTTGTCCAAGGAGATGCCGTTCGTCCACAAATGCTCAAAGAAAGCGATGTGGTC
    ATCAGTGACTTGCCTGTTGGCTATTATCCTGATGATGCCGTTGCGTCGCGCCATCAAGTT
    GCTTCTAGCCAAGAACATACTTACGCCCATCACTTGCTCATGGAACAAGGGCTTAAGTAC
    CTCAAGTCAGACGGATACGCTATTTTTCTAGCTCCGAGTGATTTGTTGACCAGTCCTCAA
    AGTGATTTGTTAAAAGAATGGCTGAAAGAAGAGGCGAGTCTGGTTGCTATGATTAGTCTG
    CCTGAAAATCTCTTTGCTAATGCTAAACAATCTAAGACTATTTTTATCCTACAGAAGAAA
    AGTGAAATAGCAGTGGAGCCTTTTGTTTATCCACTTGCTAGCTTGCAAGATGCAAGTGTT
    TTAATGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

This data comes from external databases and cannot be edited.

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SPN994039_RS09850 [old locus tag: SPN994039_17950 ]
  • symbol: SPN994039_RS09850
  • description: class I SAM-dependent methyltransferase
  • length: 317
  • theoretical pI: 4.24752
  • theoretical MW: 35718.6
  • GRAVY: -0.066877

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 20.7)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 20.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 17.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 17.3)
    and 3 more
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 15.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.8)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 37.5)
    and 5 more
    MTS; Methyltransferase small domain (PF05175; HMM-score: 19.5)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 19.3)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 18.5)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.1)
    Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002563
    • TAT(Tat/SPI): 0.00015
    • LIPO(Sec/SPII): 0.000428
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000345099 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGETELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIVEELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQAGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGLKYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFILQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI

Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Expression data[edit | edit source]

Biological Material[edit | edit source]

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

This data comes from external databases and cannot be edited.

Other Information[edit | edit source]

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]