⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 06-FEB-2015

⊟Summary

• organism: Streptococcus pneumoniae ATCC 700669
locus tag: SPN23F10040 [new locus tag: SPN23F_RS05115 ]
pan locus tag^?: PNEUPAN002248000
symbol: SPN23F10040
pan gene symbol^?: spxR
• synonym:
• product: putative DNA-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN23F10040 [new locus tag: SPN23F_RS05115 ]
• symbol: SPN23F10040
• product: putative DNA-binding protein
• replicon: chromosome
• strand: +
• coordinates: 969660..970937
• length: 1278
• essential: unknown

⊟Accession numbers

• Location: FM211187 (969660..970937) NCBI
• BioCyc: see SPN23F_RS05115
• MicrobesOnline: 5830868 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_0969 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAGTAAGCATCAGGAAATTCTAAGCTATTTGGAGGAATTACCAGTAGGTAAAAGGGTC
  AGTGTTCGTAGCATTTCGAATCATCTAGGAGTTAGTGATGGAACAGCCTATCGAGCTATT
  AAAGAAGCTGAAAACCGTGGAATTGTGGAGACCCGTCCTAGAAGTGGAACAATTCGTGTT
  AAATCCCAGAAAGTTGCTATAGAGAGATTAACGTTTGCTGAAATTGCAGAAGTGACTTCT
  TCTGAGGTTCTGGCTGGGCAAGAAGGTTTAGAGAGAGAGTTTAGTAAGTTTTCAATTGGT
  GCCATGACTGAACAAAATATCTTGTCTTACCTTCATGATGGGGGGCTCTTGATTGTCGGA
  GACCGAACCCGTATTCAGTTGCTAGCCTTGGAAAATGAAAATGCAGTTCTGGTTACAGGG
  GGATTTCAGGTTCATGATGATGTGCTTAAACTGGCCAATCAAAAAGGGATTCCTGTTCTA
  AGAAGTAAGCATGATACCTTTACCGTCGCGACCATGATCAATAAAGCCTTGTCAAATGTC
  CAAATCAAGACTGATATTCTGACAGTTGAGAAACTTTATCGCCCTAGTCATGAGTATGGT
  TTTCTGAGAGAGACAGATACAGTTAAAGATTATTTGGACTTGGTTCGTAAGAATCGTAGC
  AGCCGTTTCCCTGTTATCAATCAACATCAGGTCGTTGTTGGTGTTGTAACCATGAGAGAC
  GCTGGTGATAAATCACCAAGCACGACAATTGATAAGGTTATGTCTCGTAGTCTATTTTGG
  GTTGGATTATCGACAAATATTGCCAATGTGAGTCAACGGATGATCGCAGAAGACTTTGAA
  ATGGTACCAGTTGTTCGAAGCAATCAAACTTTGCTTGGCGTTGTGACGCGACGAGATGTC
  ATGGAGAAGATGAGCCGTTCCCAAGTTTCGGCTCTACCAACTTTTTCTGAGCAGATTGGA
  CAAAAGCTCTCTTATCACCATGATGAAGTAGTCATTACAGTGGAACCCTTTATGCTAGAA
  AAAAATGGAGTTTTGGCTAATGGTGTATTGGCAGAAATTCTGACCCACATGACCCAAGAT
  TTAGTTGTTAATAGTGGTCGCAATCTCATTATCGAGCAGATGCTGATCTACTTTTTGCAG
  GCTGTTCAGATAGATGATATATTGCGCATTCAGGCACGGATTATTCATCATACGAGACGG
  TCAGCTATAATTGATTACGATATTTATCATGGTCACCAGATTGTTTCAAAAGCAAATGTG
  ACTGTTAAAATTAATTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1278
  

⊟Protein

⊟General

• locus tag: SPN23F10040 [new locus tag: SPN23F_RS05115 ]
• symbol: SPN23F10040
• description: putative DNA-binding protein
• length: 425
• theoretical pI: 8.68924
• theoretical MW: 47722.5
• GRAVY: -0.12

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 34.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 33.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 27.7)
  and 10 more
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 26.1)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 23.3)
  tol-pal system-associated acyl-CoA thioesterase (TIGR02799; EC 3.1.2.-; HMM-score: 22.5)
  Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 19.9)
  Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 19.9)
  Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 18.7)
  Unknown function General acyl-CoA thioester hydrolase, YbgC/YbaW family (TIGR00051; HMM-score: 18.4)
  phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 14.7)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 12.2)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 12.2)
• TheSEED  :
  • FIG009439: Cytosolic protein containing multiple CBS domains
• ⊞⊞PFAM:
  MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 105.6)
  and 20 more
  no clan defined CBS; CBS domain (PF00571; HMM-score: 49.7)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 26)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 25.2)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 22.8)
  HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 22.7)
  MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 20.7)
  no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 19.6)
  HTH (CL0123) HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 19.1)
  HTH_11; HTH domain (PF08279; HMM-score: 18.8)
  MarR_2; MarR family (PF12802; HMM-score: 18.8)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 18.8)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 18.6)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 17.7)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 16.7)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 14.1)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 13.7)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 13)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.8)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.5)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004741
  • TAT(Tat/SPI): 0.001173
  • LIPO(Sec/SPII): 0.000707
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: CAR68828 NCBI
• UniProt:

⊟Protein sequence

• MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAENRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAMTEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVLRSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKNRSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIAEDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITVEPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQARIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: murZ > SPN23F10030 > SPN23F10040 > SPN23F10050

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0969 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Smirla Ramos-Montañez, Ho-Ching Tiffany Tsui, Kyle J Wayne, Jordan L Morris, Lindsey E Peters, Faming Zhang, Krystyna M Kazmierczak, Lok-To Sham, Malcolm E Winkler
Polymorphism and regulation of the spxB (pyruvate oxidase) virulence factor gene by a CBS-HotDog domain protein (SpxR) in serotype 2 Streptococcus pneumoniae.
Mol Microbiol: 2008, 67(4);729-46
[PubMed:18179423] [WorldCat.org] [DOI] (P p)