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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-AUG-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae SPN994039
  • locus tag: SPN994039_14900 [new locus tag: SPN994039_RS08060 ]
  • pan locus tag?: PNEUPAN003131000
  • symbol: engA
  • pan gene symbol?: engA
  • synonym: der
  • product: GTP-binding protein EngA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN994039_14900 [new locus tag: SPN994039_RS08060 ]
  • symbol: engA
  • product: GTP-binding protein EngA
  • replicon: chromosome
  • strand: -
  • coordinates: 1519341..1520651
  • length: 1311
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: FQ312044 (1519341..1520651) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1519 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGCCCTACCAACTATTGCCATTGTAGGACGTCCCAATGTTGGGAAATCAACCCTATTT
    AATCGGATCGCTGGTGAGCGAATCTCCATTGTAGAAGATGTCGAAGGAGTGACACGTGAC
    CGTATTTATGCAACGGGTGAGTGGCTCAATCGTTCTTTTAGCATGATTGATACAGGAGGA
    ATTGATGATGTCGATGCTCCTTTCATGGAACAAATCAAGCACCAGGCAGAAATTGCCATG
    GAAGAAGCAGATGTTATCGTTTTTGTCGTGTCTGGTAAGGAAGGAATTACTGATGCAGAC
    GAATACGTAGCTCGTAAGCTTTATAAGACCCACAAACCAGTTATCCTCGCAGTCAACAAG
    GTGGACAACCCTGAGATGCGAAATGATATCTATGATTTCTATGCCCTCGGTTTGGGTGAA
    CCACTGCCTATCTCATCTGTCCATGGTATCGGTACAGGGGATGTACTAGATGCGATTGTG
    GAAAACCTTCCAAATGAATATGAAGAAGAAAATCCAGATGTCATTAAGTTTAGCTTGATT
    GGTCGTCCTAACGTTGGAAAATCAAGCTTGATTAATGCTATCTTGGGAGAAGACCGTGTC
    ATTGCCAGTCCTGTTGCTGGAACAACTCGTGACGCTATTGATACCCACTTTACAGATACA
    GATGGTCAAGAGTTTACCATGATTGATACGGCTGGTATGCGTAAGTCTGGTAAGGTTTAT
    GAAAATACTGAGAAGTACTCTGTCATGCGTGCCATGCGTGCTATTGACCGTTCAGATGTG
    GTCTTAATGGTCATCAATGCGGAAGAGGGGATTCGTGAATACGACAAACGTATCGCTGGA
    TTTGCTCATGAAGCTGGTAAAGGGATGATTATCGTGGTCAACAAGTGGGATACGCTTGAA
    AAAGATAACCACACTATGAAAAACTGGGAAGAAGATATCCGTGAGCAGTTCCAATACCTG
    CCTTACGCACCGATTATCTTTGTATCAGCTTTAACCAAGCAACGTCTCCACAAACTTCCT
    GAGATGATTAAGCAAATCAGCGAAAGTCAAAATACACGTATTCCATCAGCTGTCTTGAAC
    GATGTCATCATGGATGCCATTGCCATCAACCCAACACCGACAGACAAAGGAAAACGTCTC
    AAGATTTTCTATGCGACCCAAGTGGCAACCAAACCACCAACCTTTGTCATCTTTGTCAAT
    GAAGAAGAACTCATGCACTTTTCTTACCTGCGTTTCTTGGAAAATCAAATCCGCAAGGCC
    TTTGTTTTTGAGGGAACACCGATTCATCTCATCGCAAGAAAACGCAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1311

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN994039_14900 [new locus tag: SPN994039_RS08060 ]
  • symbol: EngA
  • description: GTP-binding protein EngA
  • length: 436
  • theoretical pI: 5.07777
  • theoretical MW: 49109.8
  • GRAVY: -0.238991

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 577.7)
    and 29 more
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 174.9)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 172.7)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 169.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 165.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 102)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 92)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 77.3)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 65.9)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 64.5)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 55.6)
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 46.6)
    Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 37.2)
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 30.1)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines GTP-binding protein (TIGR00991; HMM-score: 28.7)
    Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 26.8)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 25.2)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 23.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines chloroplast protein import component Toc86/159, G and M domains (TIGR00993; HMM-score: 23.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 20.5)
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 17.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 14.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 14.4)
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 13.7)
    Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 12.5)
    Hypothetical proteins Conserved TIGR00703 family protein (TIGR00703; HMM-score: 12.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 11.9)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • ⊞PFAM:
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 179)
    and 31 more
    no clan defined KH_dom-like; KH-domain-like of EngA bacterial GTPase enzymes, C-terminal (PF14714; HMM-score: 115.5)
    P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 99.8)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 83.7)
    AIG1; AIG1 family (PF04548; HMM-score: 68.8)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 58.3)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 42.5)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 34.1)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 31.2)
    ABC_tran; ABC transporter (PF00005; HMM-score: 29.9)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 27.4)
    Ras; Ras family (PF00071; HMM-score: 25.1)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 23.6)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.8)
    AAA_18; AAA domain (PF13238; HMM-score: 19.6)
    DUF5906; Family of unknown function (DUF5906) (PF19263; HMM-score: 19.5)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 18.4)
    P-loop_NTPase (CL0023) NTPase_1; NTPase (PF03266; HMM-score: 17.8)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.6)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 17.3)
    Nribosyltransf (CL0498) Nuc_deoxyri_tr2; Nucleoside 2-deoxyribosyltransferase like (PF15891; HMM-score: 16.7)
    P-loop_NTPase (CL0023) NB-ARC; NB-ARC domain (PF00931; HMM-score: 16.5)
    DO-GTPase2; Double-GTPase 2 (PF19993; HMM-score: 15.7)
    TniB; Bacterial TniB protein (PF05621; HMM-score: 15.4)
    MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 15.3)
    AAA_24; AAA domain (PF13479; HMM-score: 15.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 14.6)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.5)
    vWA-like (CL0128) DUF1194; Protein of unknown function (DUF1194) (PF06707; HMM-score: 14.3)
    P-loop_NTPase (CL0023) Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 13.9)
    AAA_22; AAA domain (PF13401; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9861
    • Cytoplasmic Membrane Score: 0.0089
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.005
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004411
    • TAT(Tat/SPI): 0.00032
    • LIPO(Sec/SPII): 0.000491
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CCP35184 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYATGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEYVARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIVENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFTDTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDKRIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQRLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKPPTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1519 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPN994039_14900&oldid=153369"
  • This page was last edited on 20 January 2022, at 18:58.
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