⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 25-OCT-2019

⊟Summary

• organism: Streptococcus pneumoniae ATCC 49619
locus tag: SPAT_0393 [new locus tag: EL263_RS02120 ]
pan locus tag^?: PNEUPAN001273000
symbol: liaR
pan gene symbol^?: vraR
• synonym:
• product: Transcriptional regulatory protein LiaR

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAT_0393 [new locus tag: EL263_RS02120 ]
• symbol: liaR
• product: Transcriptional regulatory protein LiaR
• replicon: chromosome
• strand: +
• coordinates: 388521..389153
• length: 633
• essential: unknown

⊟Accession numbers

• Location: AP018938 (388521..389153) NCBI
• BioCyc: see EL263_RS02120
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0352 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAAAATTTTACTAGTAGATGACCATGAAATGGTCCGATTGGGCTTGAAAAGCTACTTT
  GACCTCCAAGACGATGTAGAAGTTGTGGGTGAGGCGTCCAACGGGTCTCAAGGGATTGAC
  TTGGCCTTGGAACTGCGTCCAGATGTCATTGTCATGGATATTGTCATGCCTGAGATGAAT
  GGGATTGACGCGACCTTAGCAATCCTTAAAGAATGGCCTGAAGCCAAGATTTTGATTGTG
  ACCTCTTATTTGGACAATGAAAAAATCATGCCAGTCTTAGATGCTGGTGCCAAAGGCTAT
  ATGCTCAAGACTTCTAGTGCAGATGAATTGCTTCATGCCGTCAGTAAGGTAGCTGCTGGC
  GAGCTGGCCATTGAGCAAGAGGTTAGCAAGAAGGTTGAATACCACCGCAATCATATGGAA
  CTTCATGAAGAATTGACTGCGCGTGAGCGAGATGTTCTCCAACTCATCGCCAAGGGCTAC
  GAAAATCAGCGCATCGCAGATGACCTCTTTATCTCTCTCAAGACGGTCAAGACCCACGTG
  TCCAACATTCTTGCCAAACTTGAAGTTAGCGATCGTACCCAGGCGGCTGTCTATGCCTTT
  CAGCACCATTTGGTCGGGCATGAGGAGTTTTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  633
  

⊟Protein

⊟General

• locus tag: SPAT_0393 [new locus tag: EL263_RS02120 ]
• symbol: LiaR
• description: Transcriptional regulatory protein LiaR
• length: 210
• theoretical pI: 4.64916
• theoretical MW: 23561.9
• GRAVY: -0.077619

⊟Function

• ⊞⊞TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 69.1)
  and 13 more
  transcriptional regulator EpsA (TIGR03020; HMM-score: 46.6)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 43.4)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 43.4)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 43.4)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 37.8)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 37.8)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 36.8)
  LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 35.3)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 25.6)
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 25.6)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 25)
  Cellular processes Cellular processes Sporulation and germination RNA polymerase sigma-H factor (TIGR02859; HMM-score: 12.8)
  Genetic information processing Transcription Transcription factors RNA polymerase sigma-H factor (TIGR02859; HMM-score: 12.8)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 90.6)
  HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 77.5)
  and 4 more
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 29.2)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 21.3)
  no clan defined DUF3071; Protein of unknown function (DUF3071) (PF11268; HMM-score: 12.8)
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002273
  • TAT(Tat/SPI): 0.000108
  • LIPO(Sec/SPII): 0.000274
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBG38450 NCBI
• UniProt:

⊟Protein sequence

• MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGYENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0352 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Vegard Eldholm, Beatrice Gutt, Ola Johnsborg, Reinhold Brückner, Patrick Maurer, Regine Hakenbeck, Thorsten Mascher, Leiv Sigve Håvarstein
The pneumococcal cell envelope stress-sensing system LiaFSR is activated by murein hydrolases and lipid II-interacting antibiotics.
J Bacteriol: 2010, 192(7);1761-73
[PubMed:20118250] [WorldCat.org] [DOI] (I p)