⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 30-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae G54
locus tag: SPG_0351 [new locus tag: SPG_RS01780 ]
pan locus tag^?: PNEUPAN001272000
symbol: SPG_0351
pan gene symbol^?: vraS
• synonym:
• product: histidine kinase TCS03

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPG_0351 [new locus tag: SPG_RS01780 ]
• symbol: SPG_0351
• product: histidine kinase TCS03
• replicon: chromosome
• strand: +
• coordinates: 345841..346836
• length: 996
• essential: unknown

⊟Accession numbers

• Location: CP001015 (345841..346836) NCBI
• BioCyc: see SPG_RS01780
• MicrobesOnline: 5755620 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_0351 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAAAAAACAAGCCTATGTCATTATTGCTCTCACCTCCTTCCTATTTGTCTTTTTTTTC
  TCCCACAGCTTGCTGGAAATACTTGATTTTGACTGGTCTATCTTTTTGCACGATGTCGAA
  AAAACAGAAAAATTTGTCTTTTTATTGTTGGTTTTCAGCATGTCCATGACCTGTCTCTTA
  GCCCTGTTTTGGCGAGGGATCGAAGAGCTTTCTCTAAGAAAAATGCAGGCTAATCTCAAG
  CGTTTATTGGCAGGGCAAGAAGTAGTTCAGGTTGTAGATCCAGATTTGGATGCCAGTTTC
  AAGTCCTTATCAGGTAAACTTAACCTTTTGACAGAGGCTCTTCAAAAAGCTGAAAATCAT
  AGCCTTGCTCAGGAAGAGGAAATCATCGAGAAGGAACGGAAGCGAATTGCTCGGGATTTG
  CACGATACAGTCAGTCAGGAGTTGTTTGCGGCCCACATGATTTTATCGGGTATCAGTCAG
  CAGGCTTTGAAATTGGATAGAGAAAAGATGCAGACCCAGTTGCAGAGTGTCACAGCTATT
  TTAGAAACAGCCCAGAAGGATTTGCGGGTTTTGCTCTTGCATTTGCGACCAGTTGAACTG
  GAGCAGAAGAGCTTGATAGAAGGGATTCAAATTCTCTTAAAAGAGCTTGAGGACAAGAGT
  GATCTTAGGGTTAGTCTCAAGCAGAATATGACGAAATTGCCTAAGAAAATCGAGGAGCAT
  ATCTTCCGTATCCTGCAAGAGTTGATTAGCAATACGCTCCGCCATGCCCAGGCATCTTGC
  CTAGATGTCTACCTCTATCAGACAGATGTTGAATTGCAACTGAAGGTGGTGGACAATGGG
  ATTGGTTTCCAGTTAGGGAGCTTAGACGACTTGAGTTATGGACTGCGAAATATCAAGGAT
  CGGGTTGAAGATATGGCAGGGACGGTTCAGTTATTAACAGCTCCCAAGCAAGGGCTGGCA
  GTTGATATCCGTATTCCCCTGTTAGATAAGGAATGA
  60
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  420
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  540
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  960
  996
  

⊟Protein

⊟General

• locus tag: SPG_0351 [new locus tag: SPG_RS01780 ]
• symbol: SPG_0351
• description: histidine kinase TCS03
• length: 331
• theoretical pI: 5.67703
• theoretical MW: 37925
• GRAVY: 0.0111782

⊟Function

• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 19)
• TheSEED  :
  • Sensor histidine kinase VraS
• ⊞⊞PFAM:
  His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 60.1)
  and 7 more
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 25.9)
  no clan defined TssO; Type VI secretion system, TssO (PF17561; HMM-score: 17.4)
  Cystatin (CL0121) Latexin; Latexin (PF06907; HMM-score: 11.5)
  no clan defined TEX13; Testis-expressed sequence 13 protein family (PF15186; HMM-score: 11.5)
  DUF3091; Protein of unknown function (DUF3091) (PF11291; HMM-score: 11.1)
  Sec2p; GDP/GTP exchange factor Sec2p (PF06428; HMM-score: 11)
  DinB (CL0310) DUF1993; Domain of unknown function (DUF1993) (PF09351; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.04
  • Cytoplasmic Membrane Score: 9.96
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024299
  • TAT(Tat/SPI): 0.000109
  • LIPO(Sec/SPII): 0.013656
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI:
• RefSeq: ACF55019 NCBI
• UniProt:

⊟Protein sequence

• MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKSLSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQQALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKDRVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mvaK1 > mvaD > mvaK2 > SPG_0349 > SPG_0350 > SPG_0351 > SPG_0352

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0351 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Vegard Eldholm, Beatrice Gutt, Ola Johnsborg, Reinhold Brückner, Patrick Maurer, Regine Hakenbeck, Thorsten Mascher, Leiv Sigve Håvarstein
The pneumococcal cell envelope stress-sensing system LiaFSR is activated by murein hydrolases and lipid II-interacting antibiotics.
J Bacteriol: 2010, 192(7);1761-73
[PubMed:20118250] [WorldCat.org] [DOI] (I p)