⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 24-APR-2024

⊟Summary

• organism: Streptococcus pneumoniae Hungary19A-6
locus tag: SPH_RS02690 [old locus tag: SPH_0553 ]
pan locus tag^?: PNEUPAN001338000
symbol: ilvC
pan gene symbol^?: ilvC
• synonym:
• product: ketol-acid reductoisomerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPH_RS02690 [old locus tag: SPH_0553 ]
• symbol: ilvC
• product: ketol-acid reductoisomerase
• replicon: chromosome
• strand: +
• coordinates: 500637..501659
• length: 1023
• essential: unknown

⊟Accession numbers

• Location: NC_010380 (500637..501659) NCBI
• BioCyc: SPH_RS02690 BioCyc
• MicrobesOnline: see SPH_0553
• PneumoBrowse for strain D39V: SPV_0406 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGACAGTTCAAATGGAATATGAAAAAGATGTTAAAGTAGCAGCACTTGACGGTAAAAAA
  ATCGCCGTTATCGGTTATGGTTCACAAGGACATGCACATGCTCAAAACTTGCGTGATTCA
  GGTCGTGACGTTATTATCGGTGTACGTCCAGGTAAATCTTTTGATAAAGCAAAAGAAGAT
  GGATTTGATACTTACACAGTAGTAGAAGCTACTAAGTTGGCTGATGTTATCATGATCTTG
  GCGCCAGACGAAATTCAACAAGAATTGTACGAAGCAGAAATCGCTCCAAACTTGGAAGCT
  GGAAACGCAGTTGGATTTGCCCATGGTTTCAACATCCACTTTGAATTTATCAAAGTTCCT
  GCGGATGTAGATGTCTTCATGTGTGCTCCTAAAGGACCAGGACACTTGGTACGTCGTACT
  TACGAAGAAGGATTTGGTGTTCCAGCTCTTTATGCAGTATACCAAGATGCAACAGGAAAT
  GCTAAAAACATTGCTATGGACTGGTGTAAAGGTGTTGGAGCGGCTCGTGTAGGTCTTCTT
  GAAACAACTTACAAAGAAGAAACTGAAGAAGATTTGTTTGGTGAACAAGCTGTACTTTGT
  GGTGGTTTGACTGCCCTTATCGAAGCAGGTTTCGAAGTCTTGACAGAAGCAGGTTACGCT
  CCAGAATTGGCTTACTTTGAAGTTCTTCACGAAATGAAATTGATCGTTGACTTGATCTAC
  GAAGGTGGATTCAAGAAAATGCGTCAATCTATTTCAAACACTGCTGAATACGGTGACTAT
  GTATCAGGTCCACGTGTAATCACTGAACAAGTTAAAGAAAATATGAAGGCTGTCTTGGCA
  GACATCCAAAATGGTAAATTTGCAAATGACTTTGTAAATGACTATAAAGCTGGACGTCCA
  AAATTGACTGCTTACCGTGAACAAGCAGCTAACCTTGAAATTGAAAAAGTTGGTGCAGAA
  TTGCGTAAAGCAATGCCATTCGTTGGTAAAAACGACGATGATGCATTCAAAATCTATAAC
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1023
  

⊟Protein

⊟General

• locus tag: SPH_RS02690 [old locus tag: SPH_0553 ]
• symbol: IlvC
• description: ketol-acid reductoisomerase
• length: 340
• theoretical pI: 4.57001
• theoretical MW: 37378.1
• GRAVY: -0.215588

⊟Function

• ⊞⊞reaction:
  EC 1.1.1.86?  ExPASy
  Ketol-acid reductoisomerase (NADP⁺) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 444)
  and 5 more
  Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 25.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 16.7)
  2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 12.9)
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 12.6)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 11.4)
• TheSEED: see SPH_0553
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 251.9)
  6PGD_C (CL0106) IlvC; Acetohydroxy acid isomeroreductase, catalytic domain (PF01450; HMM-score: 211.9)
  and 10 more
  NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 27.6)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 25.9)
  AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 22.9)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 22.7)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 17)
  no clan defined DUF1103; Repeat of unknown function (DUF1103) (PF06513; HMM-score: 16.3)
  NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 15.3)
  CoA_binding; CoA binding domain (PF02629; HMM-score: 14.6)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 12.5)
  FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.040638
  • TAT(Tat/SPI): 0.002691
  • LIPO(Sec/SPII): 0.00366
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000218063 NCBI
• UniProt: see SPH_0553

⊟Protein sequence

• MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDVIIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGNAVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGNAKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAGYAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMKAVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDDAFKIYN

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPH_RS02680 > ilvN > ilvC

⊟Regulation

• regulator: CodY see SPH_0553

⊟Expression data

• PneumoExpress for strain D39V: SPV_0406 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications