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serotype 4
D39
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D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
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serotype 3
AP200
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serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1109 [new locus tag: SPR_RS05535 ]
  • pan locus tag?: PNEUPAN002497000
  • symbol: fhs
  • pan gene symbol?: fhs
  • synonym:
  • product: Formate--tetrahydrofolate ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1109 [new locus tag: SPR_RS05535 ]
  • symbol: fhs
  • product: Formate--tetrahydrofolate ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1109867..1111537
  • length: 1671
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAAAACAGATATTGAAATCGCACAGAGTATTGAGTTGAAGCCAATTGTTGATGTTGTA
    GAGAAACTTGGTATTTCTTACGACGATTTGGAGTTGTACGGAAAGTACAAGGCTAAACTC
    AGCTTTGATAAAATTCGTGCAGTTGAGAGCAATCCAGTCGGTAAATTGATCTTGGTTACT
    GCCATCAACCCAACACCTGCAGGTGAAGGAAAGTCGACGCTTACCATTGGTCTTGCGGAT
    GCCTTGAACAAGATTGGCAAGAAAACCATGATTGCTATCCGCGAACCGTCTCTTGGTCCA
    GTCATGGGGATCAAGGGTGGTGCTGCTGGTGGTGGGTATGCACAAGTTCTGCCAATGGAA
    GACATCAACCTCCACTTTACTGGAGATATGCATGCTATTACAACTGCCAACAATGCCCTT
    TCTGCCTTGATTGACAACCACTTGCACCAAGGGAATGAGCTGGGAATTGATCAACGTCGT
    ATCCTCTGGAAACGCGTTGTGGACTTGAACGACCGTGCGCTCCGCCATGTGACTGTTGGT
    CTTGGTGGTCCTCTAAACGGTATTCCACGTGAGGACGGTTTTGATATTACAGTTGCTTCA
    GAAATCATGGCAATTCTTTGCTTGGCAACGGACATCGAGGACTTGAAACGTCGTTTGGCG
    AATATCGTTATTGGTTATCGCTATGACCGTACGCCTGTTTCTGTAGGTGATTTGCAGGTT
    GAGGGTGCCTTGGCTTTGATTTTGAAGGATGCTATTAAGCCAAACTTGGTTCAGACAATT
    TACGGTACACCTGCCTTTGTACACGGTGGTCCATTTGCCAATATTGCTCATGGCTGTAAC
    TCTGTTTTGGCGACGACAACAGCCCTTCACTTGGCTGATTACACTGTTACTGAAGCTGGT
    TTTGGTGCGGACCTTGGTGCTGAGAAATTCCTTGATATCAAGACACCAAACTTGCCAACA
    TCTCCAGATGCAGTTGTTATTGTCGCAACCCTTCGTGCCCTTAAGATGAATGGTGGTGTG
    GCTAAAGACGCTCTGACTGAAGAAAATGTAGAGGCAGTTCGTGCAGGTTTTGCTAACTTG
    AAACGCCACGTTGAAAATATCCGTAAGTTCGGTATTCCAGCAGTTGTAGCTATTAACGAA
    TTTGTATCTGATACAGAAGCTGAAATTGCAGTCTTAAAAGAACTCTGTGCCTCAATCGAT
    GTACCAGTTGAATTGGCTAGTGTCTGGGCTGATGGAGCAGAAGGTGGAGTAGCACTTGCC
    GAAACAGTTGTTAAGACAATTGCTGAAAATCCAGCTAACTATAAACGTTTGTATGATAAT
    GACCTTTCTGTCCAAGAAAAGATTGAAAAAATTGTCACTGAAATCTATCGTGGTAGCAAA
    GTGAACTTTGAGAAGAAATCTCAAACACAAATTGCTCAAATCGTTCAAAACGGTTGGGAC
    AAATTGCCAATCTGTATGGCTAAAACTCAATACAGTTTCTCAGATAATCCAAATGCGCTT
    GGAGCACCAGAAAACTTTGAAATTACCATTCGTGAATTGGTACCAAAATTAGGTGCAGGC
    TTCATCGTTGCCTTAACTGGTGATGTTATGACCATGCCAGGTCTTCCAAAACGTCCAGCA
    GCTCTCAACATGGATGTTGAAAGCGATGGAACTGTACTAGGCTTGTTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1671

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1109 [new locus tag: SPR_RS05535 ]
  • symbol: Fhs
  • description: Formate--tetrahydrofolate ligase
  • length: 556
  • theoretical pI: 5.09027
  • theoretical MW: 59629.3
  • GRAVY: 0.069964

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.4.3?  ExPASy
    Formate--tetrahydrofolate ligase ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
  • TIGRFAM:
  • ⊞TheSEED  :
    • Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    and 3 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
    Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  Formate--tetrahydrofolate ligase (EC 6.3.4.3)
  • ⊞PFAM:
    P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 878.4)
    and 2 more
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 18.4)
    no clan defined DUF719; Protein of unknown function (DUF719) (PF05334; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003959
    • TAT(Tat/SPI): 0.000607
    • LIPO(Sec/SPII): 0.000373
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDKIRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVMGIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRRILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRRLANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIAHGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRALKMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVLKELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEKIVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEITIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulators: MtaR* (repression) regulon, PurR (repression) regulon

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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