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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0149 [new locus tag: SP_RS00770 ]
  • pan locus tag?: PNEUPAN000871000
  • symbol: SP_0149
  • pan gene symbol?: metQ
  • synonym:
  • product: lipoprotein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0149 [new locus tag: SP_RS00770 ]
  • symbol: SP_0149
  • product: lipoprotein
  • replicon: chromosome
  • strand: +
  • coordinates: 146497..147351
  • length: 855
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE005672 (146497..147351) NCBI
  • BioCyc: see SP_RS00770
  • MicrobesOnline: 115690 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0151 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAATCAAAAAATGGCTTGGTCTAGCAGCCCTTGCTACAGTCGCAGGTTTGGCTCTT
    GCAGCTTGCGGAAACTCAGAAAAGAAAGCAGACAATGCAACAACTATCAAAATCGCAACT
    GTTAACCGTAGCGGTTCTGAAGAAAAACGTTGGGACAAAATCCAAGAATTGGTTAAAAAA
    GACGGAATTACCTTGGAATTTACAGAGTTCACAGACTACTCACAACCAAACAAAGCAACT
    GCTGATGGCGAAGTAGATTTGAACGCTTTCCAACACTATAACTTCTTGAACAACTGGAAC
    AAAGAAAACGGAAAAGACCTTGTAGCGATTGCAGATACTTACATCTCTCCAATCCGCCTT
    TACTCAGGTTTGAATGGAAGTGCCAACAAGTACACTAAAGTAGAAGACATCCCAGCAAAC
    GGAGAAATCGCTGTACCGAATGACGCTACAAACGAAAGCCGTGCGCTTTATTTGCTTCAA
    TCAGCTGGCTTGATTAAATTGGATGTTTCTGGAACTGCTCTTGCAACAGTTGCCAACATC
    AAAGAAAATCCAAAGAACTTGAAAATCACTGAATTGGACGCTAGCCAAACAGCTCGTTCA
    TTGTCATCAGTTGACGCTGCCGTTGTAAACAATACCTTCGTTACAGAAGCAAAATTGGAC
    TACAAGAAATCACTTTTCAAAGAACAAGCTGATGAAAACTCAAAACAATGGTACAACATC
    ATTGTTGCAAAAAAAGATTGGGAAACATCACCTAAGGCTGATGCTATCAAGAAAGTAATC
    GCAGCTTACCACACAGATGACGTGAAAAAAGTTATCGAAGAATCATCAGATGGTTTGGAT
    CAACCAGTTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    855

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0149 [new locus tag: SP_RS00770 ]
  • symbol: SP_0149
  • description: lipoprotein
  • length: 284
  • theoretical pI: 5.26389
  • theoretical MW: 31176.8
  • GRAVY: -0.439437

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 168.9)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 10.9)
  • ⊞TheSEED  :
    • Methionine ABC transporter substrate-binding protein
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine ABC transporter substrate-binding protein
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine ABC transporter substrate-binding protein
  • ⊞PFAM:
    PBP (CL0177) Lipoprotein_9; NlpA lipoprotein (PF03180; HMM-score: 306.3)
    and 2 more
    no clan defined ATP19; ATP synthase subunit K (PF11022; HMM-score: 16)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.001531
    • TAT(Tat/SPI): 0.000108
    • LIPO(Sec/SPII): 0.998158
    • Cleavage Site: CS pos: 22-23. LAA-CG. Pr: 0.9988
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AAK74331 NCBI
  • UniProt: A0A0H2UN92 UniProt

⊟Protein sequence[edit | edit source]

  • MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRSGSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKENGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQSAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAKLDYKKSLFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESSDGLDQPVW

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SP_0149 > SP_0150 > SP_0151 > SP_0152

⊟Regulation[edit | edit source]

  • ⊞regulator: MtaR* (repression) regulon
    MtaR*(TF)important in Methionine metabolism; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0151 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_0149&oldid=2369"
  • This page was last edited on 20 January 2022, at 13:10.
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