⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 15-OCT-2021

⊟Summary

• organism: Streptococcus pneumoniae P1031
locus tag: SPP_RS02215 [old locus tag: SPP_0446 ]
pan locus tag^?: PNEUPAN001284000
symbol: SPP_RS02215
pan gene symbol^?: fabM
• synonym:
• product: enoyl-CoA hydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPP_RS02215 [old locus tag: SPP_0446 ]
• symbol: SPP_RS02215
• product: enoyl-CoA hydratase
• replicon: chromosome
• strand: +
• coordinates: 400218..401003
• length: 786
• essential: unknown other strains

⊟Accession numbers

• Location: NC_012467 (400218..401003) NCBI
• BioCyc: SPP_RS02215 BioCyc
• MicrobesOnline: see SPP_0446
• PneumoBrowse for strain D39V: SPV_0378 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  
  ATGGAACACATTATTTATCAGCTTGAAGAGGACTTGGCAATCCTTACCTTGAACCGTCCT
  GAGGTCGCAAATGGTTTTCATATTCCCATGTGTGAGGAGATTTTAGAAGCTCTGACTTTG
  GCAGAAGAAAATCCAGCTGTGCATTTTATCTTAATCAATGCCAATGGAAAGGTCTTCTCA
  GTTGGGGGAGATTTGGTAGAGATGAAGCGGGCAGTGGATGAGGATGATATTCCATCATTG
  ACAAAAATCGCAGAGTTGGTCAATACGATTTCTTATAAAATCAAGCAAATAGCCAAACCT
  GTTTTAATGGAAGTTGACGGTGCTGTTGCAGGTGCCGCAGCGAATATGGCTGTTGCGGCA
  GATTTCTGTTTGGCGACGGATAAGGCTAAGTTTATCCAAGCCTTTGTTGGTGTTGGTTTG
  GCTCCAGATGCAGGGGGAATTCATCTCTTGAGCCGCAGTATCGGTGTGACGCGTGCTGCT
  CAATTAGCTATGACAGGAGAGGCTTTAACGGCAGAAAAAGCCCTAGAATGGGGGTTGGTT
  TACCGCGTTTCTGAAGCTGAAAAACTTGAAAAGACGAGAGAACAGCTTCTTAAAAAATTA
  AGACGTGCTTCAAGTAATTCCTATGCTGCCATTAAGAAGTTGGTTTGGGAGAGCCAGTTT
  AAAGACTGGCAGGGTTACGCTACTTTAGAACTGAACCTACAGAAATCCTTAGCTCAAACA
  GAGGATTTCAAAGAAGGAGTGCGAGCTCATTCGGAGAGAAGAAGACCTAAATTTACAGGA
  AAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  786
  

⊟Protein

⊟General

• locus tag: SPP_RS02215 [old locus tag: SPP_0446 ]
• symbol: SPP_RS02215
• description: enoyl-CoA hydratase
• length: 261
• theoretical pI: 6.0624
• theoretical MW: 28722.9
• GRAVY: -0.0743295

⊟Function

• ⊞⊞reaction:
  EC 4.2.1.17?  ExPASy
  Enoyl-CoA hydratase (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H₂O
• ⊞⊞TIGRFAM:
  phenylacetate degradation probable enoyl-CoA hydratase PaaB (TIGR02280; EC 4.2.1.17; HMM-score: 194.7)
  and 8 more
  2-ketocyclohexanecarboxyl-CoA hydrolase (TIGR03210; EC 3.7.-.-; HMM-score: 93.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 89.6)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadB (TIGR02437; EC 1.1.1.35,4.2.1.17,5.1.2.3,5.3.3.8; HMM-score: 72)
  fatty acid oxidation complex, alpha subunit, mitochondrial (TIGR02441; EC 1.1.1.35,4.2.1.17; HMM-score: 50.8)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty oxidation complex, alpha subunit FadJ (TIGR02440; EC 1.1.1.35,4.2.1.17,5.1.2.3; HMM-score: 47.1)
  6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase (TIGR03200; EC 3.-.-.-; HMM-score: 46.9)
  cyclohexa-1,5-dienecarbonyl-CoA hydratase (TIGR03189; EC 4.2.1.100; HMM-score: 46.2)
  benzoyl-CoA-dihydrodiol lyase (TIGR03222; EC 4.1.2.44; HMM-score: 21.5)
• TheSEED: see SPP_0446
• ⊞⊞PFAM:
  ClpP_crotonase (CL0127) ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 165.7)
  and 2 more
  ECH_2; Enoyl-CoA hydratase/isomerase (PF16113; HMM-score: 120.1)
  Peptidase_S49; Peptidase family S49 (PF01343; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002776
  • TAT(Tat/SPI): 0.000107
  • LIPO(Sec/SPII): 0.000199
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000400073 NCBI
• UniProt:

⊟Protein sequence

• MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEENPAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVLMEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAAQLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWESQFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPP_RS02215 > SPP_RS02220 > SPP_RS02225 > SPP_RS02230 > fabK > fabD > fabG > fabF > accB > fabZ > accC > accD > SPP_RS02275

⊟Regulation

• regulator: FabT* see SPP_0446

⊟Expression data

• PneumoExpress for strain D39V: SPV_0378 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications