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serotype 4
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serotype 19A
Hu17
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INV200
serotype 14
JJA
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MDRSPN001
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NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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OXC141
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R6
serotype 2
SP49
serotype 19A
SPN032672
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SPN034156
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SPN034183
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SPN994038
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SPNA45
serotype 3
ST556
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TCH8431/19A
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serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0832 [new locus tag: SPR_RS04175 ]
  • pan locus tag?: PNEUPAN001912000
  • symbol: proB
  • pan gene symbol?: proB
  • synonym:
  • product: Gamma-glutamyl kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0832 [new locus tag: SPR_RS04175 ]
  • symbol: proB
  • product: Gamma-glutamyl kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 832944..834074
  • length: 1131
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATAATCGGAGTGAGACTATGAAATACAAACGGATTGTCTTTAAGGTGGGTACTTCT
    TCTCTGACAAATGAGGATGGAAGTTTATCACGTAGTAAGGTAAAGGATATTACCCAGCAG
    TTGGCTATGCTGCACGAGGCTGGTCATGAGTTGATTTTGGTGTCTTCAGGTGCCATTGCG
    GCTGGTTTTGGAGCCTTAGGATTTAAAAAGCGTCCGACTAAAATTGCTGATAAACAGGCT
    TCAGCAGCGGTAGGGCAGGGACTTTTGTTGGAAGAATATACGACCAATCTTCTCTTGCGT
    CAAATCGTTTCTGCACAAATCTTGCTGACCCAAGATGACTTTGTGGATAAGCGTCGTTAT
    AAAAATGCCCATCAGGCTTTGTCGGTTTTGCTCAACCGTGGGGCAATTCCTATCATCAAT
    GAGAATGATAGTGTCGTTATTGATGAGCTCAAGGTTGGGGACAATGACACTCTAAGTGCT
    CAAGTAGCGGCGATGGTCCAAGCAGACCTTTTAGTTTTCTTGACAGATGTGGACGGTCTC
    TATACTGGAAATCCTAATTCAGATCCAAGAGCCAAACGCTTGGAGAGAATCGAGACCATC
    AATCGTGAGATTATTGATATGGCTGGTGGAGCTGGTTCGTCAAACGGAACTGGGGGTATG
    TTAACCAAAATCAAGGCTGCAACTATCGCGACGGAATCAGGAGTTCCTGTTTATATCTGC
    TCATCCTTGAAATCAGATTCCATGATTGAGGCGGCAGAGGAGACCGAGGATGGTTCTTAC
    TTTGTTGCTCAAGAGAAGGGGCTTCGTACCCAGAAACAATGGCTTGCCTTCTATGCTCAG
    AGTCAAGGTTCTATTTGGGTTGATAAAGGGGCTGCGGAAGCTCTCTCTCAACATGGAAAG
    AGTCTTCTCTTATCTGGTATCGTTGAAGCAGAAGGAGTCTTTTCTTACGGTGATATCGTG
    ACAGTATTTGATAAGGAAAGTGGAAAATCACTTGGAAAAGGACGCGTGCAATTTGGAGCA
    TCTGCTTTGGAGGATATATTGCGTTCTCAAAAAGCCAAGGGTGTCTTGATTTACCGTGAC
    GACTGGATTTCCATTACTCCTGAAATCCAACTACTTTTTACAGAATTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1131

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0832 [new locus tag: SPR_RS04175 ]
  • symbol: ProB
  • description: Gamma-glutamyl kinase
  • length: 376
  • theoretical pI: 5.31551
  • theoretical MW: 40716
  • GRAVY: -0.128191

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.11?  ExPASy
    Glutamate 5-kinase ATP + L-glutamate = ADP + L-glutamate 5-phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 447.6)
    and 10 more
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 156.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 45.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.4)
    Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 36.3)
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 35.9)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 34.4)
    arCOG04150 universal archaeal PUA-domain protein (TIGR03684; HMM-score: 31.4)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 29.7)
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 22.2)
    uncharacterized domain 2 (TIGR00451; HMM-score: 17)
  • TheSEED  :
    • Glutamate 5-kinase (EC 2.7.2.11)
    • RNA-binding C-terminal domain PUA
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline Synthesis  Glutamate 5-kinase (EC 2.7.2.11)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 143.6)
    and 1 more
    PUA (CL0178) PUA; PUA domain (PF01472; HMM-score: 59.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9673
    • Cytoplasmic Membrane Score: 0.01
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0223
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.037049
    • TAT(Tat/SPI): 0.001841
    • LIPO(Sec/SPII): 0.001777
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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