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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1825 [new locus tag: SPR_RS09240 ]
  • pan locus tag?: PNEUPAN003647000
  • symbol: gapA
  • pan gene symbol?: gap
  • synonym:
  • product: Glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1825 [new locus tag: SPR_RS09240 ]
  • symbol: gapA
  • product: Glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)
  • replicon: chromosome
  • strand: -
  • coordinates: 1799954..1801033
  • length: 1080
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGATTTGTAGTGATTCAAGTTACTCTTTTCACAATAAAAATTTCATGATTTTCATAAGG
    AGGAAATCACTAATGGTAGTTAAAGTTGGTATTAACGGTTTCGGACGTATCGGTCGTCTT
    GCTTTCCGTCGTATCCAAAACGTAGAAGGTGTTGAAGTTACACGCATCAACGACCTTACA
    GATCCAGTTATGCTTGCACACTTGTTGAAATACGACACAACTCAAGGTCGTTTCGACGGT
    ACTGTTGAAGTTAAAGAAGGTGGATTTGAAGTTAACGGTAAATTCATCAAAGTTTCTGCT
    GAACGTGATCCAGAACAAATCGACTGGGCTACTGACGGTGTAGAAATCGTTCTTGAAGCT
    ACTGGTTTCTTTGCTAAGAAAGAAGCAGCTGAAAAACACCTTAAAGGTGGAGCTAAAAAA
    GTTGTTATCACTGCTCCTGGTGGAAACGACGTTAAAACAGTTGTATTCAACACTAACCAC
    GACGTTCTTGACGGTACTGAAACAGTTATCTCAGGTGCTTCATGTACTACAAACTGCTTG
    GCTCCAATGGCTAAAGCTCTTCAAGACAACTTTGGTGTTGTTGAAGGATTGATGACTACT
    ATCCACGCTTACACTGGTGACCAAATGATCCTTGACGGACCACACCGTGGTGGTGACCTT
    CGCCGTGCTCGCGCTGGTGCTGCAAACATCGTTCCTAACTCAACTGGTGCTGCAAAAGCT
    ATCGGTCTTGTAATCCCAGAATTGAATGGTAAACTTGATGGATCTGCACAACGCGTTCCA
    ACTCCAACTGGATCAGTTACTGAATTGGTAGCAGTTCTTGAAAAGAACGTTACTGTTGAT
    GAAGTGAACGCAGCTATGAAAGCAGCTTCAAACGAATCATACGGTTACACAGAAGATCCA
    ATCGTATCTTCAGATATCGTAGGTATGTCTTACGGTTCATTGTTTGACGCAACTCAAACT
    AAAGTTCTTGACGTTGACGGTAAACAATTGGTTAAAGTTGTATCATGGTACGACAACGAA
    ATGTCATACACTGCACAACTTGTTCGTACTCTTGAATACTTCGCAAAGATTGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1825 [new locus tag: SPR_RS09240 ]
  • symbol: GapA
  • description: Glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)
  • length: 359
  • theoretical pI: 5.99764
  • theoretical MW: 38762.9
  • GRAVY: -0.105014

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.12?  ExPASy
    Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 436)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 258.1)
  • TheSEED  :
    • Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (EC 1.2.1.12)
    Carbohydrates Central carbohydrate metabolism Entner-Doudoroff Pathway  NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
    and 5 more
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Pyridoxine Pyridoxin (Vitamin B6) Biosynthesis  NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
    Stress Response Oxidative stress Glutaredoxins  NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
    Virulence, Disease and Defense Virulence, Disease and Defense - no subcategory Streptococcus pyogenes Virulome  NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)
    Virulence, Disease and Defense Virulence, Disease and Defense - no subcategory Streptococcus pyogenes Virulome  Plasmin(ogen) receptor
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 207.2)
    and 2 more
    NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 121.1)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005953
    • TAT(Tat/SPI): 0.002664
    • LIPO(Sec/SPII): 0.000746
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MICSDSSYSFHNKNFMIFIRRKSLMVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHLLKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAKKEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMAKALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDPIVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIAK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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