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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: spr1884 [new locus tag: SPR_RS09605 ]
  • pan locus tag?: PNEUPAN003754000
  • symbol: guaA
  • pan gene symbol?: guaA_2
  • synonym:
  • product: Glutamine amidotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr1884 [new locus tag: SPR_RS09605 ]
  • symbol: guaA
  • product: Glutamine amidotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1860650..1861387
  • length: 738
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGAGTGATATGATAGGACTATCGTTAGCATTCGAAAGGAGAGGCATCATGGCTAGAACG
    GTTGTAGGAGTTGCTGCAAATCTATGTCCCGTAGACGCAGAAGGCAAAATCATTCATTCA
    TCTGTATCTTGTAGATTCGCAGAGATCATTCGTCAAGTCGGTGGTCTCCCTTTAGTCATT
    CCTGTTGGTGATGAGTCAGTTGTACGTGATTATGTGGAAATGATTGACAAACTCATTTTG
    ACAGGAGGCCAAAATGTTCATCCTCAGTTTTATGGAGAGAAAAAGACCGTCGAGAGCGAT
    GATTACAATCTGGTCCGTGACGAATTTGAATTGGCACTCTTGAAGGAAGCGCTTCGTCAG
    AATAAACCAATTATGGCAATCTGTCGCGGTGTCCAACTTGTCAATGTTGCCTTTGGTGGA
    ACCCTCAATCAAGAAATCGAAGGTCACTGGCAAGGACTACCTTTCGGGACATCTCACTCT
    ATTGAGACAGTAGAAGGAAGCGTGGTGGCTAAGCTATTTGGGAAAGAAAGTCAGGTCAAC
    TCAGTCCATCGTCAAAGCATTAAAGATTTGGCACCTAATTTCCGTGTAACTGCTATTGAT
    TCAAGAGACCAAACTATTGAAGCGATTGAGTCTATCGACGAGCACCGCATTATCGGTTTA
    CAGTGGCATCCAGAGTTTCTGGTTAATGAAGAAGATGGCAATTTAGAATTATTTGAGTAT
    TTATTGAATGAACTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    738

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr1884 [new locus tag: SPR_RS09605 ]
  • symbol: GuaA
  • description: Glutamine amidotransferase
  • length: 245
  • theoretical pI: 4.59535
  • theoretical MW: 27269.9
  • GRAVY: -0.0640816

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 44.7)
    and 6 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 30)
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 24.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 23.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 18.5)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 16.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 11.8)
  • TheSEED  :
    • Glutamine amidotransferase, class I
  • PFAM:
    Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 227)
    and 3 more
    GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 60.2)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 15.7)
    HemS_ChuX (CL0312) HemS; Haemin-degrading HemS.ChuX domain (PF05171; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: Signal peptide TAT(Tat/SPI) length 33 aa
    • SP(Sec/SPI): 0.143968
    • TAT(Tat/SPI): 0.43945
    • LIPO(Sec/SPII): 0.007835
    • Cleavage Site: CS pos: 33-34. VDA-EG. Pr: 0.3602
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDMIGLSLAFERRGIMARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVIPVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEALRQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKESQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGNLELFEYLLNEL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CmbR* (activation (repression)) regulon
    CmbR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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