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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 17-DEC-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Xen35
  • locus tag: CXP32_03025 [new locus tag: CXP32_RS03025 ]
  • pan locus tag?: PNEUPAN001499000
  • symbol: CXP32_03025
  • pan gene symbol?: vncS
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: CXP32_03025 [new locus tag: CXP32_RS03025 ]
  • symbol: CXP32_03025
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 570133..571461
  • length: 1329
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP025256 (570133..571461) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0525 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAACGAACAGGTTTATTTGCAAAGATATTTATCTATACCTTCTCGATATTTAGTGTT
    CTGGTTATCTGCCTTCATTTAGCTATTTATTTTCTTTTTCCTTCGACTTATCTGAGTCAT
    CGTCAGGAAACCATTGGTCAAAAGGCAACAGCCATTGCCCAGTCCCTAGAAGGGAAAGAT
    AGGCAGAGTATCGAGCAAGTGTTAGACTTGTATTCCCAGACTAGTGATATCAAGGGGACC
    GTCAAAGGTGAGATGACCGAGGACAAGTTAGAAGTCAAGGACAGTCTTCCTCTGGACACA
    GACCGCCAGACAACCTCTCTCTTTATTGAGGAGCGCGAGGTGAAAACGCAAGACGGTGGT
    ACTATGATTCTCCAGTTTCTAGCTTCCATGGATTTACAAAAGGAAGCGGAGCAAATCAGT
    CTCCAGTTTCTTCCCTATACCTTGCTGGCCTCCTTTCTGATTTCCCTTTTGGTGGCCTAC
    ATCTACGCTCGGACTATTGTTGCACCGATTTTGGAAATCAAGCGGGTGACCCGTCGGATG
    ATGGACCTGGATTCCCAAGTGCGATTGCGCGTGGATTCTAAGGATGAGATAGGTAATCTC
    AAGGAACAAATCAATAGCCTCTACCAGCATCTCTTGACTGTTATTGCGGACTTGCATGAA
    AAGAATGAAGCCATTCTCCAGCTGGAGAAGATGAAGGTCGAATTCCTACGAGGAGCTTCT
    CATGAATTGAAAACACCGCTGGCTAGTTTGAAAATCCTAATCGAAAATATGAGAGAGAAT
    ATCGGTCGTTATAAGGATAGAGACCAGTATCTGGGAGTTGCCTTGGGGATTGTGGATGAA
    CTCAATCACCATGTTCTGCAGATACTTTCCCTCTCTTCTGTGCAGGAATTGCGAGATGAT
    AGGGAAACAATTGACCTCCTCCAGATGACGCAAAATCTGGTCAAAGATTATGCCTTGCTA
    GCCAAGGAAAGAGAGCTCCAGATAGACAATAGTTTGACCCATCAGCAGGCTTATCTAAAC
    CCATCAGTTATGAAGTTGATTCTTTCTAATCTCATCAGCAATGCCATTAAGCACTCTGTT
    CCAGGTGGCTTAGTTCGAATTGGAGAAAGAGAAGGAGAACTTTTTATCGAAAATAGCTGT
    AGCTCAGAGGAACAAGAAAAACTAGCCCAGTCTTTTTCTGACAATGCCAGTCGCAAGGTC
    AAGGGGTCTGGTATGGGGCTCTTTGTGGTTAAGAGTCTATTAGAACATGAAAAATTAGCT
    TATCGTTTCGAGATGGAGGAGAATAGTTTAACCTTCTTTATAGATTTTCCAAAAGTCGTC
    CAAGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1329

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: CXP32_03025 [new locus tag: CXP32_RS03025 ]
  • symbol: CXP32_03025
  • description: sensor histidine kinase
  • length: 442
  • theoretical pI: 5.38063
  • theoretical MW: 50455.8
  • GRAVY: -0.146154

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 76)
    and 5 more
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 58)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 39.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 22)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 22)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin ATP:cob(I)alamin adenosyltransferase (TIGR00636; EC 2.5.1.17; HMM-score: 10.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 35.7)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 35.1)
    and 5 more
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 27.2)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 17.6)
    no clan defined DUF6694; Family of unknown function (DUF6694) (PF20404; HMM-score: 13)
    TssO; Type VI secretion system, TssO (PF17561; HMM-score: 10.6)
    SbcD_C; Type 5 capsule protein repressor C-terminal domain (PF12320; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.94
    • Cytoplasmic Membrane Score: 2.06
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9992
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0007
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014193
    • TAT(Tat/SPI): 0.000848
    • LIPO(Sec/SPII): 0.014979
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AUF84450 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQETIGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDRQTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAYIYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADLHEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALGIVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTHQQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSFSDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

R Novak, B Henriques, E Charpentier, S Normark, E Tuomanen
Emergence of vancomycin tolerance in Streptococcus pneumoniae.
Nature: 1999, 399(6736);590-3
[PubMed:10376600] [WorldCat.org] [DOI] (P p)Gregory T Robertson, Jingyong Zhao, Bhushan V Desai, William H Coleman, Thalia I Nicas, Raymond Gilmour, Leo Grinius, Donald A Morrison, Malcolm E Winkler
Vancomycin tolerance induced by erythromycin but not by loss of vncRS, vex3, or pep27 function in Streptococcus pneumoniae.
J Bacteriol: 2002, 184(24);6987-7000
[PubMed:12446649] [WorldCat.org] [DOI] (P p)Wolfgang Haas, Jack Sublett, Deepak Kaushal, Elaine I Tuomanen
Revising the role of the pneumococcal vex-vncRS locus in vancomycin tolerance.
J Bacteriol: 2004, 186(24);8463-71
[PubMed:15576796] [WorldCat.org] [DOI] (P p)

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