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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 17-DEC-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Xen35
  • locus tag: CXP32_07250 [new locus tag: CXP32_RS07250 ]
  • pan locus tag?: PNEUPAN002740000
  • symbol: CXP32_07250
  • pan gene symbol?: trmH
  • synonym: rlmB
  • product: 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: CXP32_07250 [new locus tag: CXP32_RS07250 ]
  • symbol: CXP32_07250
  • product: 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB
  • replicon: chromosome
  • strand: +
  • coordinates: 1373557..1374285
  • length: 729
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP025256 (1373557..1374285) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1286 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAACAAATGATATTGTCTATGGTGTCCACGCCGTTACCGAAGCCCTCCTTGCAAAT
    ACAGGAAACAAACTCTACCTCCAAGAAGATCTCCGAGGTAAGAATGTTGAGAAAGTCAAG
    GAACTAGCTACAGAAAAGAAGGTGTCCATTTCTTGGACATCAAAAAAATCTCTCTCTGAG
    ATTACTGAAGGTGCTGTTCATCAAGGTTTTGTTCTACGAGTGTCTGAATTTGCCTATAGC
    GAGCTAGATTACATCCTTGCAAAAACACGCCAAGAAGAAAATCCACTTCTATTGATTCTA
    GATGGTCTAACCGATCCCCATAATCTGGGTTCTATCTTGCGAACAGCCGATGCGACCAAT
    GTTTCAGGTGTCATCATTCCCAAGCACCGTACTGTCGGAGTAACTCCTGTCGTTGCCAAA
    ACAGCCACAGGTGCTATTGAACACGTTCCAATTGCCCGAGTGACCAACCTCAGTCAAACC
    TTAGATAAACTTAAGGATGAAGGTTTCTGGACCTTTGGAACGGATATGAACGGTACTCCT
    TGCCACAAGTGGAATACAAAAGGGAAAATCGCCCTCATCATTGGAAATGAAGGAAAAGGT
    ATCTCTAGCAACATCAAAAAACAGGTCGATGAAATGATTACCATTCCGATGAATGGACAT
    GTTCAAAGCCTTAATGCCAGTGTTGCTGCGGCCATTCTCATGTACGAAGTTTTCCGAAAT
    AGACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    729

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: CXP32_07250 [new locus tag: CXP32_RS07250 ]
  • symbol: CXP32_07250
  • description: 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB
  • length: 242
  • theoretical pI: 8.94325
  • theoretical MW: 26520.3
  • GRAVY: -0.147107

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 3 (TIGR00186; HMM-score: 265.3)
    and 2 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (cytidine(34)-2'-O)-methyltransferase (TIGR00185; EC 2.1.1.207; HMM-score: 43.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, TrmH family, group 1 (TIGR00050; EC 2.1.1.-; HMM-score: 32.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 146.2)
    and 2 more
    PELOTA (CL0101) SpoU_sub_bind; RNA 2'-O ribose methyltransferase substrate binding (PF08032; HMM-score: 58.8)
    no clan defined DUF1694; Protein of unknown function (DUF1694) (PF07997; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9868
    • Cytoplasmic Membrane Score: 0.0064
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0065
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013822
    • TAT(Tat/SPI): 0.000501
    • LIPO(Sec/SPII): 0.004653
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AUF85186 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELATEKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDGLTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQTLDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMNGHVQSLNASVAAAILMYEVFRNRL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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