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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7466
  • locus tag: DQM66_RS00710 [old locus tag: NCTC7466_00129 ]
  • pan locus tag?: PNEUPAN000796000
  • symbol: mnmG
  • pan gene symbol?: mnmG
  • synonym:
  • product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: DQM66_RS00710 [old locus tag: NCTC7466_00129 ]
  • symbol: mnmG
  • product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
  • replicon: chromosome
  • strand: +
  • coordinates: 132799..134712
  • length: 1914
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_LS483374 (132799..134712) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0129 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATTTATCATTTTACTGAAGAATACGATATTATTGTAATTGGTGCGGGACACGCTGGG
    GTTGAGGCTTCCTTGGCCGCTAGCCGTATGGGCTGTAAGGTCCTGCTTGCGACCATCAAT
    ATTGAAATGCTGGCTTTCATGCCTTGTAATCCCTCTATCGGTGGTTCTGCCAAGGGGATT
    GTCGTACGTGAAGTCGATGCCCTCGGTGGCGAGATGGCCAAGACTATTGACAAGACTTAC
    ATCCAGATGAAGATGCTCAACACAGGGAAGGGGCCAGCTGTCCGTGCTCTTCGTGCGCAG
    GCTGACAAGGAACTTTACTCTAAGGAGATGCGCAAGACAGTTGAAAATCAAGAAAATCTG
    ACCCTTCGTCAAACCATGATTGATGAGATTTTGGTGGAAGATGGCAAGGCTGTCGGTGTG
    CGTACAGCCACCCATCAAGAATATGCTGCTAAGGCTGTTATTGTGACGACAGGGACTGCT
    CTCCGTGGGGAAATTATCATCGGAGACCTCAAGTACTCATCAGGTCCTAACCACAGCTTG
    GCTTCTATTAACCTAGCTGACAATCTCAAGGAACTGGGTCTCGAAATCGGTCGTTTCAAG
    ACAGGAACCCCTCCACGTGTCAAGGCTTCTTCTATCAATTACGATGTGACAGAAATTCAG
    CCAGGAGACGAAGTGCCTAATCATTTCTCATACACTTCACGTGATGAGGATTATGTCAAG
    GACCAAGTACCATGCTGGTTGACCTATACCAATGGTACCAGTCATGAGATTATCCAAAAC
    AACCTCCACCGTGCGCCTATGTTTACAGGTGTGGTCAAGGGAGTGGGACCTCGTTACTGT
    CCATCGATTGAGGACAAGATTGTGCGCTTTGCGGACAAGGAACGTCACCAACTCTTCCTT
    GAGCCAGAAGGACGCAATACAGAGGAAGTCTATGTTCAAGGACTTTCAACCAGTCTGCCT
    GAGGATGTCCAACGTGACTTGGTTCATTCCATCAAAGGTCTGGAAAATGCAGAGATGATG
    CGAACAGGTTATGCTATTGAGTATGATATGGTCTTGCCTCATCAGTTGCGTGCGACTTTG
    GAAACCAAGAAAATCTCAGGTCTCTTCACTGCTGGTCAGACAAATGGAACGTCAGGTTAC
    GAAGAGGCAGCAGGCCAAGGGATTATCGCGGGTATCAATGCGGCTCTGAAAATCCAAGGC
    AAGCCTGAATTGATTCTAAAACGAAGTGACGGTTATATCGGGGTGATGATCGACGACTTG
    GTGACCAAGGGAACCATTGAACCTTACCGTCTCTTGACCAGTCGTGCTGAATATCGTCTC
    ATTCTTCGCCATGACAATGCTGATATGCGCTTGACTGAGATGGGCCGTGAGATTGGCCTT
    GTGGACGATGAACGCTGGGCTCGTTTTGAAATCAAGAAAAATCAATTTGATAATGAGATG
    AAGCGTCTAGACAGTATCAAACTTAAGCCAGTCAAGGAAACCAATGCTAAGGTTGAGGAA
    ATGGGCTTCAAGCCGTTGACAGATGCGGTGACAGCCAAGGAATTCCTTCGCCGTCCAGAA
    GTTTCTTACCAAGATGTGGTGGCCTTCATCGGACCAGCTGCAGAGGAATTAGATGACAAG
    ATTATCGAATTGATTGAAACAGAAATCAAGTACGAAGGCTATATTTCCAAAGCCATGGAT
    CAGGTTGCCAAGATGAAACGTATGGAAGAAAAACGCATTCCAGCCAATATCGACTGGGAT
    GACATCGATTCTATTGCGACGGAAGCTCGTCAGAAGTTCAAACTCATCAATCCAGAAACC
    ATCGGCCAAGCCAGTCGTATTTCGGGAGTAAACCCAGCAGATATTTCTATTTTGATGGTG
    TATCTGGAAGGTAAAAATCGTAGTATTTCTAAAACTCTTCAAAAATCAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1914

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: DQM66_RS00710 [old locus tag: NCTC7466_00129 ]
  • symbol: MnmG
  • description: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG
  • length: 637
  • theoretical pI: 5.76377
  • theoretical MW: 71043.9
  • GRAVY: -0.362166

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 909.1)
    and 25 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 68.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 27.4)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 24.1)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 18.9)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 18.9)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 17.3)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 15.4)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 14.9)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 14)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 13.5)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 13.4)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 13.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 12.4)
    Unknown function Enzymes of unknown specificity GMC family mycofactocin-associated oxidreductase (TIGR04542; EC 1.-.-.-; HMM-score: 12.3)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.6)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11.6)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 11.4)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 11.2)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 9.9)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 9.9)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 9.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin 3B synthase CobZ (TIGR02485; HMM-score: 9.7)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 9.6)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 9.4)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 6.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 549.7)
    and 9 more
    no clan defined GIDA_C; tRNA modifying enzyme MnmG/GidA C-terminal domain (PF13932; HMM-score: 253.2)
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 24.5)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 22.2)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 19.7)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 18.8)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 18.7)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 15.3)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9081
    • Cytoplasmic Membrane Score: 0.0108
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0811
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.026049
    • TAT(Tat/SPI): 0.009125
    • LIPO(Sec/SPII): 0.009721
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000639151 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTALRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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