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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-NOV-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae EF3030
  • locus tag: EF3030_RS06125 [old locus tag: EF3030_06125 ]
  • pan locus tag?: PNEUPAN002528000
  • symbol: carB
  • pan gene symbol?: carB
  • synonym:
  • product: carbamoyl-phosphate synthase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EF3030_RS06125 [old locus tag: EF3030_06125 ]
  • symbol: carB
  • product: carbamoyl-phosphate synthase large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1173631..1176807
  • length: 3177
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP035897 (1173631..1176807) NCBI
  • BioCyc: EF3030_RS06125 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1131 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCTAAACGTACTGATATTCAAAAAATTATGGTGATTGGTTCTGGTCCGATTATTATT
    GGTCAGGCTGCTGAGTTTGACTACGCTGGGACTCAGGCTTGCTTGTCGTTGAAAGAGGAA
    GGTTATGAGGTTGTTTTGGTTAACTCAAATCCTGCAACCATCATGACGGACAAGGAAATT
    GCTGACAAGGTTTACATTGAACCGATTACACTTGAGTTTGTGACACGTATTCTTCGTAAG
    GAAGGTCCAGATGCCTTGCTACCAACACTCGGTGGTCAGACAGGTCTCAACATGGCCATG
    GAATTATCTAAAAATGGTATCCTAGACGAGCTTGGCGTTGAACTTCTGGGTACCAAATTA
    TCTGCCATTGACCAAGCGGAGGACCGTGATCTCTTTAAACAATTGATGGAAGAGCTTGAA
    CAACCCATTCCAGAATCTGAAATTGTTAACACAGTAGAAGAAGCAGTTGCCTTTGCAGCG
    ACAATTGGCTACCCAGTCATCGTTCGTCCAGCCTTTACCCTTGGTGGTACTGGTGGTGGT
    ATGTGTGCCAACGAGAAAGAATTGCGTGAAATCACTGAAAATGGTTTGAAATTGTCACCT
    GTTACCCAATGTTTGATTGAGCGTTCGATTGCCGGTTTCAAGGAAATTGAATACGAAGTG
    ATGCGTGACTCAGCTGACAATGCCTTGGTTGTTTGTAACATGGAAAACTTTGACCCAGTT
    GGGATTCACACAGGGGATTCCATTGTATTTGCCCCTGCGCAAACCATGTCAGACTATGAA
    AACCAAATGCTTCGTGATGCGAGCTTGAGCATTATTCGTGCCCTCAAGATTGAAGGTGGA
    TGTAATGTTCAGCTAGCTCTTGATCCTAACAGCTTCAAGTATTATGTTATCGAAGTAAAC
    CCTCGTGTATCACGTTCGTCAGCCCTTGCTTCTAAAGCGACAGGATATCCGATTGCTAAG
    TTGGCTGCCAAGATTGCAGTAGGTTTGACCTTGGATGAGGTTATAAACCCAGTTACAGGT
    TCAACCTATGCCATGTTTGAACCAGCCCTTGACTACGTAGTTGCTAAGATTCCACGTTTC
    CCATTTGACAAGTTTGAAAAGGGTGAACGCCGTCTCGGTACACAAATGAAGGCGACTGGA
    GAAGTCATGGCAATCGGTCGTAACATCGAGGAATCTCTTCTCAAGGCCTGCCGCTCCCTT
    GAAATTGGGGTGCACCACAATGAAATACCTGAACTTGCAGCAGTTTCTGATGATACCTTG
    ATTGAAAAGGTTGTCAAAGCCCAAGATGACCGTCTCTTCTACGTATCAGAAGCCATTCGT
    CGTGGCTACACACCTGAAGAAATTGCAGAGCTTACAAAAATTGATATCTTCTATCTTGAT
    AAACTCTTGCATATCTTTGAAATTGAGCAAGAATTGGGTGCTCATCCACAAGATCTAGAA
    GTTTTGAAAACAGCCAAACTTAATGGTTTTTCAGACCGTAAGATTGCTGAACTCTGGGGA
    ACGACTGATGATAAAGTTCGCCAACTTCGCTTGGAAAACAAGATTGTTCCAGTTTATAAG
    ATGGTAGACACTTGTGCGGCAGAGTTCGATTCCGAAACGCCATATTTCTATTCAACCTAT
    GGTTGGGAAAATGAATCTATCAAATCTGATAAAGAATCTGTACTTGTCCTAGGTTCAGGT
    CCAATCCGTATCGGTCAAGGGGTTGAGTTTGACTACGCAACCGTTCACTCTGTTAAGGCT
    ATCCAGGCAGCTGGTTATGAAGCTATCATCATGAACTCAAACCCAGAGACCGTTTCAACA
    GACTTCTCTGTATCAGATAAGCTTTACTTTGAGCCATTGACATTCGAAGATGTTATGAAC
    GTCATTGACTTGGAACAACCAAAGGGAGTTATCGTTCAGTTCGGTGGTCAAACAGCCATC
    AACCTTGCAGAGCCATTGGCAAAAGCAGGTGTGACCATCCTTGGTACACAGGTTGCTGAC
    CTAGACCGTGCCGAAGACCGTGACCTCTTCGAGCAAGCTCTCAAAGAATTGGATATTCCA
    CAGCCACCAGGACAAACGGCTACCAATGAAGAAGAAGCAGCTCTTGCAGCTCGTAAGATT
    GGCTTCCCAGTTCTTGTCCGCCCATCTTATGTACTTGGTGGACGTGCCATGGAAATCGTT
    GAAAACGAAGAAGACCTTCGTTCTTACATGCGAACTGCTGTTAAGGCTAGTCCAGACCAT
    CCAGTTCTTGTCGACTCTTATATCGTTGGGCAAGAGTGCGAAGTTGATGCCATTTCAGAT
    GGTAAAAATGTACTCATTCCTGGTATCATGGAGCATATCGAACGTGCTGGTGTCCACTCA
    GGTGACTCAATGGCTGTTTACCCACCACAAACCTTGTCGCAAAAGGTTCAGGAGACCATC
    GCAGACTACACCAAACGCTTAGCAATCGGTCTTCACTGCCTTGGAATGATGAACATCCAG
    TTTGTTATCAAGGATGAAAAAGTCTACGTTATTGAGGTCAATCCACGTGCCAGCCGTACC
    GTTCCATTCCTATCTAAGGTAACCAATATTCCTATGGCTCAAGTAGCGACCAAGCTCATT
    CTTGGTCAAAGTTTATCAGAACTTGGCTACCAAAATGGACTTTACCCTGAAAGCACCCGT
    GTTCATATCAAGGCACCTGTCTTCTCCTTTACCAAACTAGCTAAGGTAGACAGCTTGCTC
    GGTCCTGAAATGAAGTCAACAGGTGAAGTTATGGGTTCTGATGCTACTTTGGAAAAAGCT
    CTCTATAAAGCCTTTGAAGCTTCTTATCTACACTTGCCAACTTTTGGTAATGTCGTCTTT
    ACCATCGCTGATGATGCCAAAGAAGAAGCCTTGAACTTGGCTCGTCGTTTCCAAAACATT
    GGTTATGGAATCCTTGCGACAGAAGGGACAGCAGCCTTCTTTGCTAGTCATGGCTTGCAA
    GCCCAACCTGTTGGTAAGATTGGTGATGACGATAAGGATATCCCAAGTTTTGTACGCAAA
    GGAAGAATTCAAGCTATCATTAACACAGTAGGAACAAAACGAACTGCTGACGAAGATGGT
    GAGCAAATTCGTCGTTCAGCCATTGAACACGGGGTGCCCCTCTTCACAGCGCTAGATACA
    GCTAATGCCATGCTTAAGGTACTTGAAAGTCGTAGCTTTGTTACAGAAGCGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3177

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EF3030_RS06125 [old locus tag: EF3030_06125 ]
  • symbol: CarB
  • description: carbamoyl-phosphate synthase large subunit
  • length: 1058
  • theoretical pI: 4.51893
  • theoretical MW: 116060
  • GRAVY: -0.0881853

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.5.5?  ExPASy
    Carbamoyl-phosphate synthase (glutamine-hydrolyzing) 2 ATP + L-glutamine + HCO3(-) + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 1548)
    and 13 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 114.3)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 90.3)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 84.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 84.2)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 74.5)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 59.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 55.3)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 54.3)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 48.9)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 48.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 45.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme gamma-F420-2:alpha-L-glutamate ligase (TIGR04443; EC 6.3.2.32; HMM-score: 30.6)
    ATP-grasp enzyme, GAK system (TIGR04356; HMM-score: 19.2)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 390.5)
    and 14 more
    HTH (CL0123) CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerisation domain (PF02787; HMM-score: 82.6)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 73.3)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 69.2)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 64.9)
    no clan defined MGS; MGS-like domain (PF02142; HMM-score: 63.4)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 53.9)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 40)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 34.4)
    RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 31.5)
    ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 26.9)
    ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 17.7)
    ATPgrasp_ST; Sugar-transfer associated ATP-grasp (PF14397; HMM-score: 15.3)
    no clan defined DUF4176; Domain of unknown function (DUF4176) (PF13780; HMM-score: 14.4)
    FGAR-AT_N; Formylglycinamide ribonucleotide amidotransferase N-terminal (PF18076; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8638
    • Cytoplasmic Membrane Score: 0.1092
    • Cell wall & surface Score: 0.0031
    • Extracellular Score: 0.0238
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.108033
    • TAT(Tat/SPI): 0.004968
    • LIPO(Sec/SPII): 0.021554
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001126409 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEVVLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMELSKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAATIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEYEVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALKIEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEVINPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEESLLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAELTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQLRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQGVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDLEQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQPPGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDHPVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQETIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVATKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSDATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAAFFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEHGVPLFTALDTANAMLKVLESRSFVTEAI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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