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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ATCC 49619
  • locus tag: EL263_RS02035 [old locus tag: SPAT_0378 ]
  • pan locus tag?: PNEUPAN001208000
  • symbol: EL263_RS02035
  • pan gene symbol?: rlmL
  • synonym:
  • product: THUMP domain-containing class I SAM-dependent RNA methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL263_RS02035 [old locus tag: SPAT_0378 ]
  • symbol: EL263_RS02035
  • product: THUMP domain-containing class I SAM-dependent RNA methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 372760..373917
  • length: 1158
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018938 (372760..373917) NCBI
  • BioCyc: EL263_RS02035 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0341 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAAAGAATTTAATTTAATTGCAACTGTGGCAGCAGGGCTTGAGGCTGTCGTTGGT
    CGTGAAGTGCGAGAGTTGGGCTACGATTGTCAGGTTGAAAATGGACGTGTTCGTTTTCAA
    GGAGACGTGAGAGCTATTATCGAAACCAACCTTTGGCTTCGGGCAGCAGATCGTATCAAA
    ATTATCGTAGGAACGTTCCCAGCTAAGACTTTTGAAGAGCTATTTCAGGGAGTTTTCGCT
    TTAGATTGGGAAAATTATTTACCACTTGGAGCTCGGTTCCCGATTTCAAAAGCTAAATGT
    GTTAAGTCCAAACTTCACAATGAGCCCAGTGTTCAGGCTATTTCTAAGAAAGCTGTTGTC
    AAGAAATTGCAGAAACACTATGCTCGCCCAGAAGGGGTTCCTCTGATGGAGAATGGCCCA
    GAGTTTAAGATTGAGGTCTCTATTCTCAAAGATGTGGCAACTGTCATGATTGATACGACC
    GGGTCTAGCCTCTTTAAACGTGGTTATCGTACCGAAAAAGGTGGCGCTCCTATCAAGGAA
    AATATGGCAGCAGCCATTTTACAACTTTCTAACTGGTATCCAGACAAGCCTTTGATTGAT
    CCGACCTGTGGTTCGGGGACTTTCTGTATTGAGGCAGTTATGATTGCTAGAAAGATGGCG
    CCAGGTCTTCGTCGCTCTTTTGCATTTGAGGAATGGAACTGGATCAGCGATCGCTTGATT
    CAAGAAGTGCGCACAGAAGCGGCTAAAAAAGTAGAACGTGAGCTTGAGTTGGATATCATG
    GGCTGTGATATTGATGCTCGCATGGTGGAAATTGCTAAGGCCAATGCTCAGGCAGCTGGT
    GTTGCAGGAGATATTACTTTTAAGCAGATGCGCGTGCAGGATTTACGTTCCGATAAAATC
    AATGGAGTAATCATTTCCAATCCGCCTTATGGTGAACGTTTGTCAGATGATGCAGGAGTG
    ACCAAGCTCTATGCTGAGATGGGGCAAGTATTTGCACCGCTGAAAACTTGGAGCAAATTT
    ATCCTGACTAGTGATGAAGCTTTTGAAAGCAAGTATGGTAGCCAAGCAGATAAGAAGCGT
    AAGTTATACAACGGAACCTTGAAAGTGGATCTATATCAATATTTTGGTCAGCGTGTCAAA
    CGGCAAGAGGTAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1158

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL263_RS02035 [old locus tag: SPAT_0378 ]
  • symbol: EL263_RS02035
  • description: THUMP domain-containing class I SAM-dependent RNA methyltransferase
  • length: 385
  • theoretical pI: 9.46182
  • theoretical MW: 43259.8
  • GRAVY: -0.283896

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 38.8)
    and 6 more
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 25.5)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 25.2)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 19.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 16.2)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 14.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 183.6)
    and 8 more
    no clan defined THUMP; THUMP domain (PF02926; HMM-score: 34.4)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 27.6)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 25.6)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 24.4)
    N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 19.1)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.2)
    MmeI_Mtase; MmeI, DNA-methyltransferase domain (PF20473; HMM-score: 11.6)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9731
    • Cytoplasmic Membrane Score: 0.0027
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0238
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.065935
    • TAT(Tat/SPI): 0.003312
    • LIPO(Sec/SPII): 0.034811
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000711391 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVRAIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVKSKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTTGSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARKMAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANAQAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPLKTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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