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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NU83127
  • locus tag: EL277_RS00670 [old locus tag: SPNU_0122 ]
  • pan locus tag?: PNEUPAN000792000
  • symbol: pspA
  • pan gene symbol?: pspA
  • synonym:
  • product: pneumococcal surface protein A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL277_RS00670 [old locus tag: SPNU_0122 ]
  • symbol: pspA
  • product: pneumococcal surface protein A
  • replicon: chromosome
  • strand: +
  • coordinates: 127157..129391
  • length: 2235
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018936 (127157..129391) NCBI
  • BioCyc: EL277_RS00670 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATAAGAAAAAAATGATTTTAACAAGTCTAGCCAGCGTCGCTATCTTAGGGGCTGGT
    TTTGTTACGTCTCAGCCTACTTTTGTAAGAGCAGAAGAATCTCCACAAGTTGTCGAAAAA
    TCTTCATTAGAGAAGAAATATGAGGAAGCAAAAGCAAAAGCTGATACTGCCAAGAAAGAT
    TACGAAACGGCTAAAAAGAAAGCAGAAGACGCTCAGAAAAAGTATGAAGATGATCAGAAG
    AGAACTGAGGAGAAAGCTCGAAAAGAAGCAGAAGCATCTCAAAAATTGAATGATGTGGCG
    CTTGTTGTTCAAAATGCATATAAAGAGTACCGAGAAGTTCAAAATCAACGTAGTAAATAT
    AAATCTGACGCTGAATATCAGAAAAAATTAACAGAGGTCGACTCTAAAATAGAGAAGGCT
    AGGAAAGAGCAACAGGACTTGCAAAATAAATTTAATGAAGTAAGAGCAGTTGTAGTTCCT
    GAACCAAATGCGTTGGCTGAGACTAAGAAAAAAGCAGAAGAAGCTAAAGCAGAAGAAAAA
    GTAGCTAAGAGAAAATATGATTATGCAACTCTAAAGGTAGCACTAGCGAAGAAAGAAGTA
    GAGGCTAAGGAACTTGAAATTGAAAAACTTCAATATGAAATTTCTACTTTGGAACAAGAA
    GTTGCTACTGCTCAACATCAAGTAGATAATTTGAAAAAACTTCTTGCTGGTGCGGATCCT
    GATGATGGCACAGAAGTTATAGAAGCTAAATTAAAAAAAGGAGAAGCTGAGCTAAACGCT
    AAACAAGCTGAGTTAGCAAAAAAACAAACAGAACTTGAAAAACTTCTTGACAGCCTTGAT
    CCTGAAGGTAAGACTCAGGATGAATTAGATAAAGAAGCAGAAGAAGCTGAGTTGGATAAA
    AAAGCTGATGAACTTCAAAATAAAGTTGCTGATTTAGAAAAAGAAATTAGTAACCTTGAA
    ATATTACTTGGAGGGGCTGATCCTGAAGATGATACTGCTGCTCTTCAAAATAAATTAGCT
    GCTAAAAAAGCTGAGTTAGCAAAAAAACAAACAGAACTTGAAAAACTTCTTGACAGCCTT
    GATCCTGAAGGTAAGACTCAGGATGAATTAGATAAAGAAGCAGAAGAAGCTGAGTTGGAT
    AAAAAAGCTGATGAACTTCAAAATAAAGTTGCTGATTTAGAAAAAGAAATTAGTAACCTT
    GAAATATTACTTGGAGGGGCTGATTCTGAAGATGATACTGCTGCTCTTCAAAATAAATTA
    GCTACTAAAAAAGCTGAATTGGAAAAAACTCAAAAAGAATTAGATGCAGCTCTTAATGAG
    TTAGGCCCTGATGGAGATGAAGAAGAAACTCCAGCGCCGGCTCCTCAACCAGAGCAACCA
    GCTCCTGCACCAAAACCAGAGCAACCAGCTCCAGCTCCAAAACCAGAGCAACCAGCTCCT
    GCACCAAAACCAGAGCAACCAGCTCCAGCTCCAAAACCAGAGCAACCAGCTCCAGCTCCA
    AAACCAGAGCAACCAGCTAAGCCGGAGAAACCAGCTGAAGAGCCTACTCAACCAGAAAAA
    CCAGCCACTCCAAAAACAGGCTGGAAACAAGAAAACGGTATGTGGTATTTCTACAATACT
    GATGGTTCAATGGCAATAGGTTGGCTCCAAAACAACGGTTCATGGTACTACCTAAACGCT
    AACGGCGCTATGGCAACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAAGCA
    TCAGGTGCTATGAAAGCAAGCCAATGGTTCAAAGTATCAGATAAATGGTACTATGTCAAC
    AGCAATGGCGCTATGGCGACAGGCTGGCTCCAATACAATGGCTCATGGTACTACCTCAAC
    GCTAATGGTGATATGGCGACAGGATGGCTCCAATACAACGGTTCATGGTATTACCTCAAC
    GCTAATGGTGATATGGCGACAGGATGGGCTAAAGTCAACGGTTCATGGTACTACCTAAAC
    GCTAACGGTGCTATGGCTACAGGTTGGGCTAAAGTCAACGGTTCATGGTACTACCTAAAC
    GCTAACGGTTCAATGGCAACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAA
    GCATCAGGTGCTATGAAAGCAAGCCAATGGTTCAAAGTATCAGATAAATGGTACTATGTC
    AATGGCTTAGGTGCCCTTGCAGTCAACACAACTGTAGATGGCTATAAAGTCAATGCCAAT
    GGTGAATGGGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2235

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL277_RS00670 [old locus tag: SPNU_0122 ]
  • symbol: PspA
  • description: pneumococcal surface protein A
  • length: 744
  • theoretical pI: 4.54045
  • theoretical MW: 82763.6
  • GRAVY: -0.925941

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 70.8)
    and 8 more
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 31.8)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 31.8)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 25)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 25)
    Genetic information processing DNA metabolism Restriction/modification DNA sulfur modification protein DndD (TIGR03185; HMM-score: 16.4)
    helix-rich protein (TIGR04523; HMM-score: 9.2)
    CcmD family protein (TIGR04391; HMM-score: 8.1)
    delta-60 repeat domain (TIGR02608; HMM-score: 5.2)
  • TheSEED: data available for TIGR4
  • PFAM:
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 194.8)
    Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 166.6)
    and 10 more
    Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 51.3)
    no clan defined TMF_DNA_bd; TATA element modulatory factor 1 DNA binding (PF12329; HMM-score: 50.4)
    E1-N; E1 N-terminal domain (PF14463; HMM-score: 19.8)
    OmpH; Outer membrane protein (OmpH-like) (PF03938; HMM-score: 16.7)
    Pox_A_type_inc; Viral A-type inclusion protein repeat (PF04508; HMM-score: 15.9)
    HSBP1; Heat shock factor binding protein 1 (PF06825; HMM-score: 14.5)
    FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 12.8)
    EF_hand (CL0220) EF-hand_9; EF-hand domain (PF14658; HMM-score: 8)
    no clan defined DUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 7.2)
    Cast; RIM-binding protein of the cytomatrix active zone (PF10174; HMM-score: 5.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0.9189
    • Extracellular Score: 0.0807
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.991806
    • TAT(Tat/SPI): 0.003828
    • LIPO(Sec/SPII): 0.00349
    • Cleavage Site: CS pos: 31-32. VRA-EE. Pr: 0.9672
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001035362 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLEKKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALVVQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVPEPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTAALQNKLAAKKAELAKKQTELEKLLDSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWLQNNGSWYYLNANGAMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

S Hammerschmidt, G Bethe, P H Remane, G S Chhatwal
Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae.
Infect Immun: 1999, 67(4);1683-7
[PubMed:10085004] [WorldCat.org] [DOI] (P p)A H Tu, R L Fulgham, M A McCrory, D E Briles, A J Szalai
Pneumococcal surface protein A inhibits complement activation by Streptococcus pneumoniae.
Infect Immun: 1999, 67(9);4720-4
[PubMed:10456922] [WorldCat.org] [DOI] (P p)S K Hollingshead, R Becker, D E Briles
Diversity of PspA: mosaic genes and evidence for past recombination in Streptococcus pneumoniae.
Infect Immun: 2000, 68(10);5889-900
[PubMed:10992499] [WorldCat.org] [DOI] (P p)L S McDaniel, J S Sheffield, E Swiatlo, J Yother, M J Crain, D E Briles
Molecular localization of variable and conserved regions of pspA and identification of additional pspA homologous sequences in Streptococcus pneumoniae.
Microb Pathog: 1992, 13(4);261-9
[PubMed:1363703] [WorldCat.org] [DOI] (P p)Mirza Shaper, Susan K Hollingshead, William H Benjamin, David E Briles
PspA protects Streptococcus pneumoniae from killing by apolactoferrin, and antibody to PspA enhances killing of pneumococci by apolactoferrin [corrected].
Infect Immun: 2004, 72(9);5031-40
[PubMed:15321996] [WorldCat.org] [DOI] (P p)M J Crain, W D Waltman, J S Turner, J Yother, D F Talkington, L S McDaniel, B M Gray, D E Briles
Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of Streptococcus pneumoniae.
Infect Immun: 1990, 58(10);3293-9
[PubMed:1698178] [WorldCat.org] [DOI] (P p)J Yother, D E Briles
Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis.
J Bacteriol: 1992, 174(2);601-9
[PubMed:1729249] [WorldCat.org] [DOI] (P p)L S McDaniel, B A Ralph, D O McDaniel, D E Briles
Localization of protection-eliciting epitopes on PspA of Streptococcus pneumoniae between amino acid residues 192 and 260.
Microb Pathog: 1994, 17(5);323-37
[PubMed:7723659] [WorldCat.org] [DOI] (P p)J Yother, J M White
Novel surface attachment mechanism of the Streptococcus pneumoniae protein PspA.
J Bacteriol: 1994, 176(10);2976-85
[PubMed:7910604] [WorldCat.org] [DOI] (P p)R C Tart, L S McDaniel, B A Ralph, D E Briles
Truncated Streptococcus pneumoniae PspA molecules elicit cross-protective immunity against pneumococcal challenge in mice.
J Infect Dis: 1996, 173(2);380-6
[PubMed:8568299] [WorldCat.org] [DOI] (P p)

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