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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ASP0581
  • locus tag: EL334_RS00700 [old locus tag: ASP0581_01240 ]
  • pan locus tag?: PNEUPAN000792000
  • symbol: pspA
  • pan gene symbol?: pspA
  • synonym:
  • product: pneumococcal surface protein A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL334_RS00700 [old locus tag: ASP0581_01240 ]
  • symbol: pspA
  • product: pneumococcal surface protein A
  • replicon: chromosome
  • strand: +
  • coordinates: 126687..128948
  • length: 2262
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP019192 (126687..128948) NCBI
  • BioCyc: EL334_RS00700 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAATAAGAAAAAAATGATTTTAACAAGTCTAGCCAGCGTCGCTATCTTAGGGGCTGGT
    TTTGTTACGTCTTCGCCTACTGTTGTAAGAGCAGAAGAAGCTCCACAAGTTGTCGAAAAA
    TCTTCATTAGAGAAGAAATATGAGGAAGCAAAAGCAAAATATGACGCTGCTAAGAAGGAT
    TACGACGAAGCTAAAAAGAAAGCAGCAGAAGCTCAGAAAAAATATGAGGAAGATCAGAAG
    AAAACTGAGGAGAAAGCGAAAAAAGAGAAAGAAGCTGCTAAGGAAGTAGATGATGCTAGT
    TTAGCGGTGCAAAAAGCACATGTAGAATATAGAAAAGTTCTAGATTCTCGTAATAGCTAC
    AGAAACCCGAGTGATTATGCTAAAAAATTAGCAGAAGCAGATAAAAAAATAACTGAGGAG
    ACGACTAAACTAACCAATGCTCAGACAAAATTTCAATCTATTCGAACAACAATTGTAGTT
    CCTGGACAAAGTGAGTTAGCTGAGACTAAGAAAAAAGCAGAAGAAGCTAAAGCAGAAGAA
    AAAGTAGCTAAGAGAAAATATGATTATGCAACTCTAAAGGTAGCACTAGCGAAGAAAGAA
    GTAGAGGCTAAGGAACTTGAAATTGAAAAACTTCAAGATGAAATTTCTACTTTGGAACAA
    GAAGTTGCTACTGCTCAACATCAAGTAGACAATTTGAAAAAACTTCTTGCTGGTGTGGAT
    CCTGATGATACAGAAGCTATAGAAGCTAAATTAAAAAAAGGAGAAGCTGAGCTAAACGCT
    AAACAAGCTGAGTTAGCAAAAAAACAAACAGAACTTGAAAAACTTCTTGACAGCCTTGAT
    CCTGAAGGTAAGACTCAGGATGAATTAGATAAAGAAGCTGCTGAAGCTGAGTTGAATAAA
    AAAGTTGAATCTCTTCAAAACAAAGTTGCTGATTTAGAAAAAGAAATTAGTAATCTTGAA
    ATATTACTTGGAGGGGCTGATTCTGAAGATGATACTGCTGCTCTTCAAAATAAATTAGCT
    ACTAAAAAAGCTGAATTAGCAAAAAAACAAACAGAACTTGAAAAACTTCTTGACAGCCTT
    GATCCTGAAGGTAAGACTCAGGATGAATTAGATAAAGAAGCAGAAGAAGCTGAGTTGGAT
    AAAAAAGCTGATGAACTTCAAAATAAAGTTGCTGATTTAGAAAAAGAAATTAGTAACCTT
    GAAATATTACTTGGAGGGGCTGATTCTGAAGATGATACTGCTGCTCTTCAAAATAAATTA
    GCTACTAAAAAAGCTGAATTGGAAAAAACTCAAAAAGAATTAGATGCAGCTCTTAATGAG
    TTAGGCCCTGATGGAGATGAAGAAGAAACTCCAGCGCCGGCTCCTCAACCAGAGCAACCA
    GCTCCTGCACCAAAACCAGAGCAACCAGCTCCTGCACCAAAACCAGAGCAACCAGCTCCT
    GCACCAAAACCAGAGCAACCAGCTCCAGCACCAAAACCAGAGCAACCAGCTCCAGCACCA
    AAACCAGAGCAACCAGCTCCAGCACCAAAACCAGAGCAACCAGCTAAGCCGGAGAAACCA
    GCTGAAGAGCCTACTCAACCAGAAAAACCAGCCACTCCAAAAACAGGCTGGAAACAAGAA
    AACGGTATGTGGTATTTCTACAATACGGATGGTTCAATGGCAATAGGTTGGCTCCAAAAC
    AACGGTTCATGGTACTACCTAAACGCTAACGGCGCTATGGCAACAGGTTGGGTGAAAGAT
    GGAGATACCTGGTACTATCTTGAAGCATCAGGTGCTATGAAAGCAAGCCAATGGTTCAAA
    GTATCAGATAAATGGTACTATGTCAACAGCAATGGCGCTATGGCGACAGGCTGGCTCCAA
    TACAATGGCTCATGGTACTACCTCAACGCTAATGGTGATATGGCGACAGGCTGGCTCCAA
    TACAACGGTTCATGGTATTACCTCAACGCTAATGGTGATATGGCGACAGGATGGGCTAAA
    GTCAACGGTTCATGGTACTACCTAAACGCTAACGGTGCTATGGCTACAGGTTGGGCTAAA
    GTCAACGGTTCATGGTACTACCTCAACGCTAACGGTTCAATGGCAACAGGTTGGGTGAAA
    GATGGAGATACCTGGTACTATCTTGAAGCATCAGGTGCTATGAAAGCAAGCCAATGGTTC
    AAAGTATCAGATAAATGGTACTATGTCAATGGCTTAGGTGCCCTTGCAGTCAACACAACT
    GTAGATGGCTATAAAGTCAATGCCAATGGTGAATGGGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2262

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL334_RS00700 [old locus tag: ASP0581_01240 ]
  • symbol: PspA
  • description: pneumococcal surface protein A
  • length: 753
  • theoretical pI: 4.60745
  • theoretical MW: 83192.2
  • GRAVY: -0.892962

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 70.6)
    and 9 more
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 32.6)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 32.6)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 26.6)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 26.6)
    CcmD family protein (TIGR04391; HMM-score: 9.4)
    SEC10/PgrA surface exclusion domain (TIGR04320; HMM-score: 7.8)
    Cellular processes Cellular processes Chemotaxis and motility flagellar hook-basal body complex protein FliE (TIGR00205; HMM-score: 5.6)
    delta-60 repeat domain (TIGR02608; HMM-score: 5.3)
    helix-rich protein (TIGR04523; HMM-score: 2.4)
  • TheSEED: data available for TIGR4
  • PFAM:
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 194.6)
    Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 166.4)
    and 12 more
    no clan defined TMF_DNA_bd; TATA element modulatory factor 1 DNA binding (PF12329; HMM-score: 53)
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 50.5)
    no clan defined Pox_A_type_inc; Viral A-type inclusion protein repeat (PF04508; HMM-score: 19.9)
    HSBP1; Heat shock factor binding protein 1 (PF06825; HMM-score: 16.4)
    E1-N; E1 N-terminal domain (PF14463; HMM-score: 15.5)
    DUF4094; Domain of unknown function (DUF4094) (PF13334; HMM-score: 10.4)
    TRAF_BIRC3_bd; TNF receptor-associated factor BIRC3 binding domain (PF16673; HMM-score: 10.1)
    DUF4349; Domain of unknown function (DUF4349) (PF14257; HMM-score: 9.8)
    NPV_P10; Nucleopolyhedrovirus P10 protein (PF05531; HMM-score: 7.7)
    DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 7.4)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 7.3)
    CPA_AT (CL0064) Mem_trans; Membrane transport protein (PF03547; HMM-score: 4.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.72
    • Internal Helices: 0
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.8855
    • Extracellular Score: 0.114
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.993784
    • TAT(Tat/SPI): 0.003238
    • LIPO(Sec/SPII): 0.002385
    • Cleavage Site: CS pos: 31-32. VRA-EE. Pr: 0.9715
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_126432039 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKKKMILTSLASVAILGAGFVTSSPTVVRAEEAPQVVEKSSLEKKYEEAKAKYDAAKKDYDEAKKKAAEAQKKYEEDQKKTEEKAKKEKEAAKEVDDASLAVQKAHVEYRKVLDSRNSYRNPSDYAKKLAEADKKITEETTKLTNAQTKFQSIRTTIVVPGQSELAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQDEISTLEQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQDELDKEAAEAELNKKVESLQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAELAKKQTELEKLLDSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWLQNNGSWYYLNANGAMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

S Hammerschmidt, G Bethe, P H Remane, G S Chhatwal
Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae.
Infect Immun: 1999, 67(4);1683-7
[PubMed:10085004] [WorldCat.org] [DOI] (P p)A H Tu, R L Fulgham, M A McCrory, D E Briles, A J Szalai
Pneumococcal surface protein A inhibits complement activation by Streptococcus pneumoniae.
Infect Immun: 1999, 67(9);4720-4
[PubMed:10456922] [WorldCat.org] [DOI] (P p)S K Hollingshead, R Becker, D E Briles
Diversity of PspA: mosaic genes and evidence for past recombination in Streptococcus pneumoniae.
Infect Immun: 2000, 68(10);5889-900
[PubMed:10992499] [WorldCat.org] [DOI] (P p)L S McDaniel, J S Sheffield, E Swiatlo, J Yother, M J Crain, D E Briles
Molecular localization of variable and conserved regions of pspA and identification of additional pspA homologous sequences in Streptococcus pneumoniae.
Microb Pathog: 1992, 13(4);261-9
[PubMed:1363703] [WorldCat.org] [DOI] (P p)Mirza Shaper, Susan K Hollingshead, William H Benjamin, David E Briles
PspA protects Streptococcus pneumoniae from killing by apolactoferrin, and antibody to PspA enhances killing of pneumococci by apolactoferrin [corrected].
Infect Immun: 2004, 72(9);5031-40
[PubMed:15321996] [WorldCat.org] [DOI] (P p)M J Crain, W D Waltman, J S Turner, J Yother, D F Talkington, L S McDaniel, B M Gray, D E Briles
Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of Streptococcus pneumoniae.
Infect Immun: 1990, 58(10);3293-9
[PubMed:1698178] [WorldCat.org] [DOI] (P p)J Yother, D E Briles
Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis.
J Bacteriol: 1992, 174(2);601-9
[PubMed:1729249] [WorldCat.org] [DOI] (P p)L S McDaniel, B A Ralph, D O McDaniel, D E Briles
Localization of protection-eliciting epitopes on PspA of Streptococcus pneumoniae between amino acid residues 192 and 260.
Microb Pathog: 1994, 17(5);323-37
[PubMed:7723659] [WorldCat.org] [DOI] (P p)J Yother, J M White
Novel surface attachment mechanism of the Streptococcus pneumoniae protein PspA.
J Bacteriol: 1994, 176(10);2976-85
[PubMed:7910604] [WorldCat.org] [DOI] (P p)R C Tart, L S McDaniel, B A Ralph, D E Briles
Truncated Streptococcus pneumoniae PspA molecules elicit cross-protective immunity against pneumococcal challenge in mice.
J Infect Dis: 1996, 173(2);380-6
[PubMed:8568299] [WorldCat.org] [DOI] (P p)

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