PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 14-DEC-2020

⊟Summary

• organism: Streptococcus pneumoniae NU83127
• locus tag: EL277_RS09610 [old locus tag: SPNU_1819 ]
• pan locus tag^?: PNEUPAN003395000
• symbol: EL277_RS09610
• pan gene symbol^?: msmE
• synonym:
• product: extracellular solute-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: EL277_RS09610 [old locus tag: SPNU_1819 ]
• symbol: EL277_RS09610
• product: extracellular solute-binding protein
• replicon: chromosome
• strand: -
• coordinates: 1822594..1823844
• length: 1251
• essential: unknown

⊟Accession numbers

• Location: NZ_AP018936 (1822594..1823844) NCBI
• BioCyc: EL277_RS09610 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1677 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGAAATGGTATAAGAAAGCAGGTTTTCTTTTAGTAGCTGGGGCTAGTTTACTAGGCCTA
  GCAGCTTGTGGTCAGAATAATCAATCGACTGATGGAAAGATAACGATTGAATTTTTTAAC
  CAGAAGACCGAAATGGCGGATACTTTGCAAAAAATTGTAGATAATTTTGAAAAGGATCAT
  CCTACTATTGATGTAAAACTGACGACTGTTCCGGCAGCTGGAATTGTTCTGAAGACTCGG
  ATTTTATCAGGAGATGTTCCAGATATTGTTAATATCTATCCTCAAAATATGGATTTTCAG
  GAGTGGGCAAAAGCAGGTTACTTTGCAGATATGACAGGGAAGTCCTATCTTGAGAATATT
  AAGAATGACTATGCCGAAAAGTATGCAATCAATAACAAGATTTATAGTGTACCTTTAACT
  GCTAACCTTTATGGAATCTATTACAATAAAACAAAGTTTAAAGAATTAGGACTTGAGGAA
  CCGAAGACTTTTAAAGAGTTTCAAGAGATTGTTAAAAAGATAAAAGATAGAGGGAATTCT
  CCGTTTGCAGTTGCAGGCAATGAAGGATGGACATTAAACGGTTATCACCAACTTTCTCTC
  ATTACTATTACAGGCAGTGGAGACGCAGCTAATAACTATCTTCGCTTTTCAAAACCAAAT
  GCTATTTCTGTAGATGATACTATTTTAAAAGCAGATGCAGAACGACTAGATTTGTTGGCA
  GACAATGCTCAAGATGGATGGCGTGGTGCCTCTTATAATGATGCTGTGGTAGCATTCTCG
  AATGAAAAAGCCTTGATGATGCCACAAGGATCATGGGCATTAGCGGCAATTAATCAACAG
  GATCCAAAATTTGATGTGGGGATGTTTGCCTTCCCAGGAGAAGAAGTAGGAAAAGATGTT
  ACTGTTGGTGCAGGGGACATGGCATTATCAACTTCAGCTACTACCAAACATCCTAAAGAA
  ACCGAGGAATTTATCAGCTATATGACTAGTCCAAAAGCTATGCAAGCATATTATGATGTG
  GATGGATCACCAGTTGCTGTAAAAGGTGTACAGGAAAAAGAAGATTCAGCGCTTGCAGAG
  ATTTCTAAACTAGCATTTACGGATAAACATTATGTTTGGTTGGGCCAACACTGGAATTCT
  GAAGAAGATTTTTTCAATCTAAGTGCAGGTTACTTGATGGATAAAAATCTGAAAAATATG
  GCAAACAATCTCAATGCTTTCTTTAATCCAATGAAGGCAGATTTGGACTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1251
  

⊟Protein

⊟General

• locus tag: EL277_RS09610 [old locus tag: SPNU_1819 ]
• symbol: EL277_RS09610
• description: extracellular solute-binding protein
• length: 416
• theoretical pI: 4.76267
• theoretical MW: 46149.8
• GRAVY: -0.370913

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 84.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter, N-acetylglucosamine/diacetylchitobiose-binding protein (TIGR03851; HMM-score: 83.8)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  PBP (CL0177) SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 144.9)
  SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 130.5)
  and 4 more
  SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 37.8)
  SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 25.4)
  no clan defined DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 14)
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 9.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
  • SP(Sec/SPI): 0.00078
  • TAT(Tat/SPI): 0.000111
  • LIPO(Sec/SPII): 0.998865
  • Cleavage Site: CS pos: 22-23. LAA-CG. Pr: 0.9994
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000872419 NCBI
• UniProt:

⊟Protein sequence

• MKWYKKAGFLLVAGASLLGLAACGQNNQSTDGKITIEFFNQKTEMADTLQKIVDNFEKDHPTIDVKLTTVPAAGIVLKTRILSGDVPDIVNIYPQNMDFQEWAKAGYFADMTGKSYLENIKNDYAEKYAINNKIYSVPLTANLYGIYYNKTKFKELGLEEPKTFKEFQEIVKKIKDRGNSPFAVAGNEGWTLNGYHQLSLITITGSGDAANNYLRFSKPNAISVDDTILKADAERLDLLADNAQDGWRGASYNDAVVAFSNEKALMMPQGSWALAAINQQDPKFDVGMFAFPGEEVGKDVTVGAGDMALSTSATTKHPKETEEFISYMTSPKAMQAYYDVDGSPVAVKGVQEKEDSALAEISKLAFTDKHYVWLGQHWNSEEDFFNLSAGYLMDKNLKNMANNLNAFFNPMKADLD

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1677 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications