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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-MAY-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_121 [new locus tag: HKM25_RS00610 ]
  • pan locus tag?: PNEUPAN000743000
  • symbol: HKM25_121
  • pan gene symbol?: aatA
  • synonym:
  • product: 3-beta hydroxysteroid dehydrogenase/isomerase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_121 [new locus tag: HKM25_RS00610 ]
  • symbol: HKM25_121
  • product: 3-beta hydroxysteroid dehydrogenase/isomerase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 115424..117274
  • length: 1851
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP053210 (115424..117274) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0099 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATAAAAAACTAACAGATTATGTGATTGATCTGGTGGAAATTTTAAATAAACAACAA
    AAGCAGGTTTTCTGGGGAATATTTGATATTTTCAGTATGGTGGTTTCCATCATTGTATCT
    TATATTTTATTTTATGGGCTGATTAATCCAGCACCTGTTGACTACATTATCTATACGAGT
    TTGGCCTTCTTGTTCTATCAATTGATGATTGGTTTTTGGGGGTTGAACGCGAGCATTAGT
    CGTTACAGCAAGATTACGGATTTCATGAAAATCTTTTTTGGTGTGACTGCTAGCAGTGTC
    TTGTCATATAGTATCTGTTATGCCTTCTTGCCACTCTTCTCCATCCGTTTCATCATTCTC
    TTTATCTTGTTGAGTACCTTCTTGATTTTATTGCCACGGATTACTTGGCAGTTAATCTAC
    TCCAGACGCAAAAAAGGTAGTGGTGATGGAGAACACCGTCGGACCTTCTTGATTGGTGCC
    GGTGATGGTGGGGCTCTTTTTATGGATAGTTACCAACATCCAACCAGTGAATTAGAACTG
    GTCGGTATTTTGGATAAGGATTCTAAGAAAAAGGGTCAAAAACTTGGTGGTATTCCTGTT
    TTGGGCTCTTATGACAATCTGCCTGAATTAGCCAAACGCCATCAAATCGAGCGTGTCATC
    GTTGCGATTCCGTCGCTGGATCCGTCAGAATATGAGCGTATCTTGCAGATGTGTAATAAG
    CTGGGTGTCAAATGTTACAAGATGCCTAAGGTTGAAACTGTTGTTCAGGGCCTTCACCAA
    GCAGGTACTGGCTTCCAAAAAATTGATATTACGGACCTTTTGGGTCGTCAGGAAATCCGT
    CTTGACGAATCGCGTCTGGGTGCAGAACTGACAGGTAAGACCATCTTAGTCACAGGAGCT
    GGAGGTTCAATCGGTTCTGAAATCTGTCGTCAAGTTAGTCGCTTCAATCCTGAACGCATT
    GTCTTGCTCGGTCATGGGGAAAACTCAATCTACCTTGTTTATCATGAATTGATTCGTAAG
    TTCCAAGGGATTGATTATGTACCTGTGATTGCGGACATTCAAGACTATGATCGTTTGTTG
    CAAGTCTTTGAGCAGTACAAACCTGCTATTGTTTATCATGCGGCAGCCCACAAGCATGTT
    CCTATGATGGAGCGCAATCCAAAAGAAGCCTTCAAAAACAATATCCGTGGAACTTACAAT
    GTTGCTAAGGCTGTTGATGAAGCTAAAGTGTCTAAGATGGTTATGATTTCGACAGATAAG
    GCAGTCAATCCACCAAATGTTATGGGAGCAACCAAGCGCGTGGCGGAGTTGATTGTCACT
    GGCTTTAACCAACGTAGCCAATCAACCTACTGTGCAGTTCGTTTTGGGAATGTTCTTGGT
    AGCCGTGGTAGTGTCATTCCAGTCTTTGAACGTCAGATTGCTGAAGGTGGGCCTGTAACG
    GTGACAGACTTCCGCATGACCCGTTACTTTATGACCATTCCAGAAGCTAGCCGTCTGGTT
    ATCCATGCTGGTGCTTATGCCAAAGATGGGGAAGTCTTTATCCTTGATATGGGCAAACCA
    GTCAAGATTTATGACTTGGCCAAGAAGATGGTGCTTCTAAGTGGCCACACTGAAAGTGAA
    ATTCCAATCGTTGAAGTTGGAATCCGCCCAGGTGAAAAACTCTACGAAGAACTCTTGGTA
    TCAACCGAACTCGTTGATAATCAAGTTATGGATAAGATTTTCGTTGGTAAGGTTAATGTC
    ATGCCTTTAGAATCCATCAATCAAAAGATTGGAGAGTTCCGCACTCTCAGTGGAGATGAG
    TTGAAGCAAGCTATTATCGCCTTTGCTAATCAAACAACCCACATTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1851

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_121 [new locus tag: HKM25_RS00610 ]
  • symbol: HKM25_121
  • description: 3-beta hydroxysteroid dehydrogenase/isomerase family protein
  • length: 616
  • theoretical pI: 8.86708
  • theoretical MW: 69206.2
  • GRAVY: 0.0787338

⊟Function[edit | edit source]

  • TIGRFAM:
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 195.6)
    and 18 more
    UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase (TIGR04130; EC 4.2.1.-,5.1.3.-; HMM-score: 153.8)
    exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 80.8)
    undecaprenyl-phosphate glucose phosphotransferase (TIGR03023; EC 2.7.8.-; HMM-score: 73.2)
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 63)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 53.6)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides CDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 47.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 47.6)
    NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 44.5)
    undecaprenyl-phosphate galactose phosphotransferase WbaP (TIGR03022; EC 2.7.8.6; HMM-score: 38.1)
    sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 35.3)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 29.5)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 22.3)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 17.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides GDP-mannose 4,6-dehydratase (TIGR01472; EC 4.2.1.47; HMM-score: 14.9)
    thioester reductase domain (TIGR01746; HMM-score: 14.2)
    Metabolism Central intermediary metabolism Other long-chain fatty acyl-ACP reductase (aldehyde-forming) (TIGR04058; EC 1.1.1.-; HMM-score: 13)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 12.5)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 12.2)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 395.5)
    and 14 more
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 85.2)
    CoA_binding_3; CoA-binding domain (PF13727; HMM-score: 60.4)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 58.9)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 54.9)
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 36.5)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 26.3)
    KR; KR domain (PF08659; HMM-score: 22.8)
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 21.6)
    CoA_binding; CoA binding domain (PF02629; HMM-score: 17.2)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 16)
    NAD_binding_4; Male sterility protein (PF07993; HMM-score: 15.1)
    DXP_reductoisom; 1-deoxy-D-xylulose 5-phosphate reductoisomerase (PF02670; HMM-score: 14.9)
    no clan defined DUF3842; Domain of unknown function (DUF3842) (PF12953; HMM-score: 12.9)
    PDDEXK (CL0236) PDDEXK_10; PD-(D/E)XK nuclease superfamily (PF07788; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0045
    • Cytoplasmic Membrane Score: 0.9901
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0053
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001296
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.004921
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: QJS35833 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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