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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-MAY-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_279 [new locus tag: HKM25_RS01375 ]
  • pan locus tag?: PNEUPAN001015000
  • symbol: HKM25_279
  • pan gene symbol?: spuA
  • synonym:
  • product: putative pullulanase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_279 [new locus tag: HKM25_RS01375 ]
  • symbol: HKM25_279
  • product: putative pullulanase
  • replicon: chromosome
  • strand: -
  • coordinates: 251430..255227
  • length: 3798
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP053210 (251430..255227) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0250 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGAAAAACACCATCTCACACTGAGAAAAAAATGGTCTACAGCATCCGTTCCCTCAAA
    AATGGAACTGGTTCTGTCCTTATCGGCGCAAGCCTTGTCTTGCTTGCCATGGCTACACCA
    ACTATCTCATCCGACGAAAGTACACCAACCACTAACGAACCCAACAACAGAAATACAACC
    ACCCTTGCCCAACCTCTTACTGATACAGCAGCTGGCTCTGGTAAGAACGAAAGTGATATT
    TCTTCACCTAGAAATGCAAACGCTTCCCTAGAGAAAACAGAAGAAAAACCTGCAACAGAG
    CCAACTACTTCTACTAGTCCAGTAACAACTGAAACTAAGGCAGAAGAGCCCATCGAAGAT
    AACTACTTCCGTATCCATGTCAAAAAACTTCCTGAAGAAAACAAGGATGCTCAAGGACTA
    TGGACTTGGGACGATGTTGAAAAACCATCTGAAAACTGGCCAAACGGAGCTTTGTCCTTC
    AAGGATGCCAAGAAAGATGACTACGGCTATTACCTAGATGTCAAATTAAAGGGAGAACAA
    GCCAAGAAAATTAGCTTCCTCATCAACAATACAGCTGGAAAAAATCTAACCGGCGATAAA
    TCTGTAGAAAAACTAGTTCCAAAAATGAACGAGGCTTGGTTAGACCAAGATTACAAGGTT
    TTCTCTTACGAGCCACAGCCTGCAGGAACTGTTCGCGTCAACTACTACCGCACAGATGGC
    AACTATGACAAGAAATCTCTCTGGTACTGGGGAGATGTAAAAAATCCAAGTAGCGCTCAA
    TGGCCTGACGGAACAGACTTTACGGCTACAGGCAAATATGGCCGCTATATCGACATTCCT
    CTTAATGAAGCCGCAAGAGAATTTGGATTTTTATTACTAGATGAGAGCAAACAAGGAGAC
    GACGTGAAAATCCGTAAAGAAAATTATAAGTTCACAGATTTGAAAAATCATAGCCAAATT
    TTCCTAAAAGACGATGATGAATCGATTTACACAAATCCATACTATGTCCATGATATCCGT
    ATGACAGGAGCCCAACACGTAGGCACTTCTAGCATTGAAAGTAGCTTTTCAACACTTGTC
    GGTGCTAAAAAAGAAGATATCCTCAAACACTCCAACATCACTAATCACCTAGGAAACAAG
    GTAACTATTACCGATGTTGCAATCGATGAAGCTGGTAAGAAAGTGACCTACAGCGGAGAT
    TTCTCTGACACAAAACATCCTTATACTGTTAGCTACAATTCCGACCAATTCACTACCAAA
    ACAAGCTGGCACCTGAAAGATGAGACATACAGCTATGATGGCAAACTGGGAGCTGACCTA
    AAAGAAGAAGGAAAACAAGTTGATTTGACCCTTTGGTCACCAAGTGCTGATAAGGTTTCT
    GTTGTTGTCTACGACAAGAATGACCCTGACAAAGTAGTTGGAACTGTCGCTCTCGAAAAA
    GGGGAAAGAGGAACTTGGAAACAAACTCTAGACAGCACAAACAAACTCGGAATCACAGAT
    TTCACTGGCTACTATTATCAATACCAAATCGAGCGTCAAGGTAAAACTGTTCTTGCACTC
    GATCCTTACGCTAAATCTCTTGCTGCTTGGAATAGCGACGATGCCAAAATTGACGATGCC
    CATAAAGTGGCTAAAGCCGCCTTTGTAGATCCAGCTAAACTCGGACCTCAAGACTTGACT
    TATGGTAAGATTCATAATTTCAAGACTCGTGAAGACGCCGTTATCTACGAAGCTCATGTG
    CGTGACTTCACTTCAGATCCTGCCATTGCAAAAGACTTGACCAAACCATTTGGGACTTTT
    GAAGCCTTCATTGAAAAACTAGACTATCTCAAAGACTTGGGTGTAACCCATATCCAGCTC
    CTTCCAGTCTTGTCTTACTACTTTGTCAATGAATTGAAAAACCATGAACGCTTGTCTGAC
    TACGCTTCAAGCAACAGCAACTACAACTGGGGATATGACCCTCAAAACTACTTCTCCTTG
    ACTGGTATGTACTCAAGCGATCCTAAGAATCCAGAAAAACGAATCGCAGAATTTAAAAAC
    CTCATCAACGAAATCCACAAACGTGGTATGGGAGCTATCCTAGATGTCGTTTATAACCAC
    ACAGCCAAAGTCGATATCTTTGAAGACCTAGAGCCAAACTACTACCACTTTATGGATGCC
    GATGGCACACCTCGAACTAGCTTTGGTGGTGGACGCTTGGGAACAACCCACCATATGACC
    AAACGGCTCCTAGTTGACTCTATCAAATACCTAGTTGATACCTACAAAGTGGATGGATTC
    CGTTTCGATATGATGGGAGACCATGACGCCGCTTCTATCGAAGAAGCTTACAAGGCTGCA
    CGCGCCCTCAATCCAAACCTCATCATGCTTGGTGAAGGTTGGAGAACCTATGCCGGTGAT
    GAAAACATGCCTACTAAAGCTGCTGACCAAGATTGGATGAAACATACCGATACTGTCGCT
    GTCTTTTCAGATGACATCCGTAACAACCTCAAATCTGGTTATCCAAACGAAGGTCAACCT
    GCCTTTATCACAGGTGGCAAGCGTGATGTCAACACCATCTTTAAAAATCTCATTGCTCAA
    CCAACTAACTTTGAAGCTGACAGCCCTGGAGATGTCATCCAATACATAGCAGCCCATGAT
    AACTTGACCCTCTTTGACATCATTGCCCAGTCTATCAAAAAAGACCCAAGCAAGGCTGAG
    AACTATGCTGAAATCCACCGTCGTTTACGACTTGGAAATCTCATGGTCTTGACAGCTCAA
    GGAACTCCATTTATCCACTCCGGTCAGGAATATGGACGTACTAAACAATTCCGTGACCCA
    GCCTACAAGACTCCAGTAGCAGAGGATAAGGTTCCAAACAAATCTCACTTGTTGCGTGAT
    AAGGACGGCAACCCATTTGACTATCCTTACTTCATCCATGATTCTTACGATTCTAGCGAC
    GCAGTCAACAAGTTTGACTGGACTAAGGCTACAGATGGTAAAGCTTATCCTGAAAATGTC
    AAGAGCCGTGACTATATGAAAGGTTTGATTGCCCTTCGTCAATCTACAGATGCCTTCCGA
    CTTAAGAGTCTCCAAGATATCAAAGACCGTGTCCACCTCATCACTGTCCCAGGCCAAAAT
    GGTGTGGAAAAAGAGGATGTAGTGATTGGCTACCAAATCACTGCTCCAAACGGCGATATC
    TACGCAGTCTTTGTCAATGCGGATGAAAAAGCTCGCGAATTTAATTTGGGAACTGCCTTT
    GCACATCTAAGAAATGCGGAAGTTTTGGCAGATGAAAACCAAGCAGGATCAGTCGGAATT
    GCCAACCCGAAAGGACTTGAATGGACTGAAAAAGGCTTGAAATTGAATGCCCTTACAGCT
    ACTGTTCTTCGAGTCTCTCAAAATGGAACTAGCCATGAGTCAACTGCAGAAGAGAAACCA
    GACTCAACCCCTTCCAAGCCTGAACATCAAAATGAAGCTTCTCACCCTGCACATCAAGAC
    CCAGCTCCAGAAGCTAGACCTGATTCTACTAAACCAGATGCCAAAGTAGCTGATGCGGAA
    AATAAACCTAGCCAAGCTACAGCTGATTCACAAGCTGAACAACCAGCACAAGAAGCACAA
    GCATCATCTGTAAAAGAAGCGGTTCGAAACGAATCGGTAGAAAACTCTAGCAAGGAAAAT
    ATACCTGCAACCCCAGATAAACAAGCTGAACTTCCAAATACAGGAATCAAAAACGAAAAC
    AAACTCCTATTTGCAGGAATCAGCCTCCTTGCGCTCCTTGGTCTCGGTTTCTTACTAAAA
    AATAAAAAAGAGAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3798

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_279 [new locus tag: HKM25_RS01375 ]
  • symbol: HKM25_279
  • description: putative pullulanase
  • length: 1265
  • theoretical pI: 5.48993
  • theoretical MW: 141193
  • GRAVY: -0.724111

⊟Function[edit | edit source]

  • TIGRFAM:
    pullulanase, extracellular (TIGR02102; HMM-score: 2012.3)
    and 10 more
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 410)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 250.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 151.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 95)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 33.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 33.9)
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 31.8)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.5)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 23.8)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 17.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    GHD (CL0369) SpuA_C; SpuA C-terminal (PF18033; HMM-score: 115.9)
    Transthyretin (CL0287) PUD; Bacterial pullanase-associated domain (PF03714; HMM-score: 101.1)
    and 7 more
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 75.1)
    GHD (CL0369) CBM_48; Carbohydrate-binding module 48 (Isoamylase N-terminal domain) (PF02922; HMM-score: 44.4)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 36)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 28.3)
    GHD (CL0369) Pullul_strch_C; Alpha-1,6-glucosidases, pullulanase-type, C-terminal (PF11852; HMM-score: 19.4)
    E-set (CL0159) FlgD_ig; FlgD Ig-like domain (PF13860; HMM-score: 14.5)
    no clan defined DUF3848; Protein of unknown function (DUF3848) (PF12959; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.6
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 9.38
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • SignalP: Signal peptide SP(Sec/SPI) length 44 aa
    • SP(Sec/SPI): 0.848694
    • TAT(Tat/SPI): 0.081099
    • LIPO(Sec/SPII): 0.031837
    • Cleavage Site: CS pos: 44-45. ISS-DE. Pr: 0.5835
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: QJS35991 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISSDESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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