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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-MAY-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_647 [new locus tag: HKM25_RS03150 ]
  • pan locus tag?: PNEUPAN001604000
  • symbol: HKM25_647
  • pan gene symbol?: yesM
  • synonym:
  • product: HAMP domain protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_647 [new locus tag: HKM25_RS03150 ]
  • symbol: HKM25_647
  • product: HAMP domain protein
  • replicon: chromosome
  • strand: +
  • coordinates: 620401..622065
  • length: 1665
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP053210 (620401..622065) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0575 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGTTATTTCCATCTTTCTGGTCTTTCTCATTCTCCTAGCTCTGGTTGGAACTTTCTAC
    TATCAATCAAGTTCTTCAGCCATTGAGGCCACCATTGAGGGCAACAGCCAAACGACCATC
    AGCCAGACTAGCCACTTTATTCAGTCTTATATCAAAAAACTAGAAACCACCTCGACTGGT
    TTGACCCAGCAGACGGATGTTCTGGCCTATGCTGAGAATCCCAGTCAAGACAAGGTCGAG
    GGAATCCGAGATTTGTTTTTGACCATCTTGAAGTCAGATAAGGACTTGAAAACTGTTGTG
    CTGGTGACCAAATCTGGTCAGGTCATTTCTACAGATGACAGTGTGCAGATGAAAACTTCC
    TCTGATATGATGGCTGAGGATTGGTACCAAAAGGCTATTCATCAGGGAGCTATGCCTGTT
    TTGACTCCAGCTCGTAAATCAGATAGTCAGTGGGTCATTTCTGTCACTCAAGAACTTGTT
    GATGCAAAGGGAGCCAATCTTGGTGTGCTTCGTTTGGATATTTCTTATGAAACTCTGGAA
    GCCTATCTCAATCAACTCCAGTTGGGGCAGCAGGGCTTTGCCTTCATTATCAATGAAAAC
    CATGAATTTGTCTACCATCCTCAACACACAGTTTATAGTTCGTCTAGCAAAATGGAGGCT
    ATGAAACCCTACATCGAGACAGGTCAGGGTTATACTCCTGGTCACAAATCCTACGTCAGT
    CAAGAGAAGATTGCAGGAACTGATTGGACGGTACTTGGCGTGTCATCATTGGAAAAGTTA
    GACCAGGTTCGGAGTCAGCTCTTGTGGACCTTGCTTGGGGCCAGTGTCACATCTCTTCTT
    GTCTGTCTCTGCTTAGTGTGGTTCAGTCTTAAACGCTGGATTGCTCCTTTGAAGGATTTG
    AGAGAAACCATGTTGGAAATTGCTTCTGGTGCTCAAAATCTTCGTGCCAAGGAAGTTGGT
    GCCTATGAACTGAGAGAAGTAACTCGCCAATTTAATGCCATGTTGGATCAGATTGATCAG
    TTGATGGTAGCTATTCGTAGCCAGGAAGAAACGACCCGTCAGTACCAACTTCAAGCCCTT
    TCGAGCCAGATTAATCCACATTTCCTCTATAACACTTTGGACACCATCATCTGGATGGCT
    GAATTTCATGATAGTCAGCGAGTGGTGCAGGTGACCAAGTCCTTGGCAACCTATTTCCGC
    TTGGCGCTCAATCAAGGTAAGGACTTGATTTGTCTCTCTGACGAAATCAATCATGTCCGC
    CAGTATCTCTTTATCCAGAAACAACGCTATGGAGATAAACTGGAATACGAAATTAATGAA
    AATGTTGCCTTTGATAATTTAGTCTTACCCAAGCTGGTCCTACAACCCCTTGTAGAAAAT
    GCTCTTTACCATGGCATTAAGGAAAAGGAAGGTCAGGGCCATATTAAACTTTCTGTCCAG
    AAACAGGATTCGGGATTGGTCATCCGTATTGAGGATGATGGCGTTGGCTTCCAAGATGCT
    GGTGATAGTAGTCAAAGTCAACTCAAACGTGGGGGAGTTGGTCTTCAAAATGTCGATCAA
    CGGCTCAAACTTCATTTTGGAGCCAATTACCAGATGAAGATTGATTCTAGACCCCAAAAA
    GGAACGAAAGTTGAAATATATATAAATAGAATAGAAACTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1665

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_647 [new locus tag: HKM25_RS03150 ]
  • symbol: HKM25_647
  • description: HAMP domain protein
  • length: 554
  • theoretical pI: 5.96208
  • theoretical MW: 62549.8
  • GRAVY: -0.223646

⊟Function[edit | edit source]

  • TIGRFAM:
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 41.8)
    and 5 more
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.9)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.9)
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 18.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 17.1)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 17.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 101.4)
    no clan defined His_kinase; Histidine kinase (PF06580; HMM-score: 87.6)
    and 7 more
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 35.6)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 35)
    Cache (CL0165) Cache_3-Cache_2; Cache 3/Cache 2 fusion domain (PF17201; HMM-score: 22)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 21.4)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 20)
    no clan defined DUF2829; Protein of unknown function (DUF2829) (PF11195; HMM-score: 13.2)
    KH (CL0007) KH_4; KH domain (PF13083; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.660828
    • TAT(Tat/SPI): 0.005813
    • LIPO(Sec/SPII): 0.062969
    • Cleavage Site: CS pos: 27-28. SSA-IE. Pr: 0.3778
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: QJS36359 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVISIFLVFLILLALVGTFYYQSSSSAIEATIEGNSQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSDKDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQWVISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQHTVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQLLWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELREVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFHDSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINENVAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQDAGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Clare E Blue, Tim J Mitchell
Contribution of a response regulator to the virulence of Streptococcus pneumoniae is strain dependent.
Infect Immun: 2003, 71(8);4405-13
[PubMed:12874319] [WorldCat.org] [DOI] (P p)Wouter T Hendriksen, Nuno Silva, Hester J Bootsma, Clare E Blue, Gavin K Paterson, Alison R Kerr, Anne de Jong, Oscar P Kuipers, Peter W M Hermans, Tim J Mitchell
Regulation of gene expression in Streptococcus pneumoniae by response regulator 09 is strain dependent.
J Bacteriol: 2007, 189(4);1382-9
[PubMed:17085554] [WorldCat.org] [DOI] (P p)Stephanie Hirschmann, Alejandro Gómez-Mejia, Ulrike Mäder, Julia Karsunke, Dominik Driesch, Manfred Rohde, Susanne Häussler, Gerhard Burchhardt, Sven Hammerschmidt
The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.
Microorganisms: 2021, 9(3);
[PubMed:33668344] [WorldCat.org] [DOI] (P e)Stephanie Hirschmann, Alejandro Gómez-Mejia, Thomas P Kohler, Franziska Voß, Manfred Rohde, Max Brendel, Sven Hammerschmidt
The Two-Component System 09 of Streptococcus pneumoniae Is Important for Metabolic Fitness and Resistance during Dissemination in the Host.
Microorganisms: 2021, 9(7);
[PubMed:34201716] [WorldCat.org] [DOI] (P e)

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