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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 09-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_RS03150 [old locus tag: HKM25_647 ]
  • pan locus tag?: PNEUPAN001604000
  • symbol: HKM25_RS03150
  • pan gene symbol?: yesM
  • synonym:
  • product: sensor histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_RS03150 [old locus tag: HKM25_647 ]
  • symbol: HKM25_RS03150
  • product: sensor histidine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 620374..622065
  • length: 1692
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP053210 (620374..622065) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0575 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAGCGTTCTTCTCTTTTAGTTAGAATGGTTATTTCCATCTTTCTGGTCTTTCTCATT
    CTCCTAGCTCTGGTTGGAACTTTCTACTATCAATCAAGTTCTTCAGCCATTGAGGCCACC
    ATTGAGGGCAACAGCCAAACGACCATCAGCCAGACTAGCCACTTTATTCAGTCTTATATC
    AAAAAACTAGAAACCACCTCGACTGGTTTGACCCAGCAGACGGATGTTCTGGCCTATGCT
    GAGAATCCCAGTCAAGACAAGGTCGAGGGAATCCGAGATTTGTTTTTGACCATCTTGAAG
    TCAGATAAGGACTTGAAAACTGTTGTGCTGGTGACCAAATCTGGTCAGGTCATTTCTACA
    GATGACAGTGTGCAGATGAAAACTTCCTCTGATATGATGGCTGAGGATTGGTACCAAAAG
    GCTATTCATCAGGGAGCTATGCCTGTTTTGACTCCAGCTCGTAAATCAGATAGTCAGTGG
    GTCATTTCTGTCACTCAAGAACTTGTTGATGCAAAGGGAGCCAATCTTGGTGTGCTTCGT
    TTGGATATTTCTTATGAAACTCTGGAAGCCTATCTCAATCAACTCCAGTTGGGGCAGCAG
    GGCTTTGCCTTCATTATCAATGAAAACCATGAATTTGTCTACCATCCTCAACACACAGTT
    TATAGTTCGTCTAGCAAAATGGAGGCTATGAAACCCTACATCGAGACAGGTCAGGGTTAT
    ACTCCTGGTCACAAATCCTACGTCAGTCAAGAGAAGATTGCAGGAACTGATTGGACGGTA
    CTTGGCGTGTCATCATTGGAAAAGTTAGACCAGGTTCGGAGTCAGCTCTTGTGGACCTTG
    CTTGGGGCCAGTGTCACATCTCTTCTTGTCTGTCTCTGCTTAGTGTGGTTCAGTCTTAAA
    CGCTGGATTGCTCCTTTGAAGGATTTGAGAGAAACCATGTTGGAAATTGCTTCTGGTGCT
    CAAAATCTTCGTGCCAAGGAAGTTGGTGCCTATGAACTGAGAGAAGTAACTCGCCAATTT
    AATGCCATGTTGGATCAGATTGATCAGTTGATGGTAGCTATTCGTAGCCAGGAAGAAACG
    ACCCGTCAGTACCAACTTCAAGCCCTTTCGAGCCAGATTAATCCACATTTCCTCTATAAC
    ACTTTGGACACCATCATCTGGATGGCTGAATTTCATGATAGTCAGCGAGTGGTGCAGGTG
    ACCAAGTCCTTGGCAACCTATTTCCGCTTGGCGCTCAATCAAGGTAAGGACTTGATTTGT
    CTCTCTGACGAAATCAATCATGTCCGCCAGTATCTCTTTATCCAGAAACAACGCTATGGA
    GATAAACTGGAATACGAAATTAATGAAAATGTTGCCTTTGATAATTTAGTCTTACCCAAG
    CTGGTCCTACAACCCCTTGTAGAAAATGCTCTTTACCATGGCATTAAGGAAAAGGAAGGT
    CAGGGCCATATTAAACTTTCTGTCCAGAAACAGGATTCGGGATTGGTCATCCGTATTGAG
    GATGATGGCGTTGGCTTCCAAGATGCTGGTGATAGTAGTCAAAGTCAACTCAAACGTGGG
    GGAGTTGGTCTTCAAAATGTCGATCAACGGCTCAAACTTCATTTTGGAGCCAATTACCAG
    ATGAAGATTGATTCTAGACCCCAAAAAGGAACGAAAGTTGAAATATATATAAATAGAATA
    GAAACTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1692

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_RS03150 [old locus tag: HKM25_647 ]
  • symbol: HKM25_RS03150
  • description: sensor histidine kinase
  • length: 563
  • theoretical pI: 6.38832
  • theoretical MW: 63621.2
  • GRAVY: -0.221492

⊟Function[edit | edit source]

  • TIGRFAM:
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 44.9)
    and 5 more
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.8)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.8)
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 18)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 17.1)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 17.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 101.4)
    no clan defined His_kinase; Histidine kinase (PF06580; HMM-score: 87.5)
    and 7 more
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 35.5)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 35)
    Cache (CL0165) Cache_3-Cache_2; Cache 3/Cache 2 fusion domain (PF17201; HMM-score: 22)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 21.4)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 20)
    no clan defined DUF2829; Protein of unknown function (DUF2829) (PF11195; HMM-score: 13.1)
    KH (CL0007) KH_4; KH domain (PF13083; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.361888
    • TAT(Tat/SPI): 0.003178
    • LIPO(Sec/SPII): 0.054817
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000831078 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGNSQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSDKDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQWVISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQHTVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQLLWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELREVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFHDSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINENVAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQDAGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Clare E Blue, Tim J Mitchell
Contribution of a response regulator to the virulence of Streptococcus pneumoniae is strain dependent.
Infect Immun: 2003, 71(8);4405-13
[PubMed:12874319] [WorldCat.org] [DOI] (P p)Wouter T Hendriksen, Nuno Silva, Hester J Bootsma, Clare E Blue, Gavin K Paterson, Alison R Kerr, Anne de Jong, Oscar P Kuipers, Peter W M Hermans, Tim J Mitchell
Regulation of gene expression in Streptococcus pneumoniae by response regulator 09 is strain dependent.
J Bacteriol: 2007, 189(4);1382-9
[PubMed:17085554] [WorldCat.org] [DOI] (P p)Stephanie Hirschmann, Alejandro Gómez-Mejia, Ulrike Mäder, Julia Karsunke, Dominik Driesch, Manfred Rohde, Susanne Häussler, Gerhard Burchhardt, Sven Hammerschmidt
The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.
Microorganisms: 2021, 9(3);
[PubMed:33668344] [WorldCat.org] [DOI] (P e)Stephanie Hirschmann, Alejandro Gómez-Mejia, Thomas P Kohler, Franziska Voß, Manfred Rohde, Max Brendel, Sven Hammerschmidt
The Two-Component System 09 of Streptococcus pneumoniae Is Important for Metabolic Fitness and Resistance during Dissemination in the Host.
Microorganisms: 2021, 9(7);
[PubMed:34201716] [WorldCat.org] [DOI] (P e)

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