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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 09-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 6A-10
  • locus tag: HKM25_RS00415 [old locus tag: HKM25_84 ]
  • pan locus tag?: PNEUPAN000705000
  • symbol: HKM25_RS00415
  • pan gene symbol?: agaR
  • synonym: cpsR
  • product: GntR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HKM25_RS00415 [old locus tag: HKM25_84 ]
  • symbol: HKM25_RS00415
  • product: GntR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 80652..81368
  • length: 717
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP053210 (80652..81368) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0064 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCAATTCCCAAATATCAATACATAAAAGACGAATTAAAAAATAAAATCATCTCTGGG
    CAGTTTAGCAGTGGAGATAAATTTTACACTGAAGCAGAACTCATTTCAATGTACGATGTC
    AGCTCTATCACAGTTGTTCGCGCCTTAAACGACCTTGCCAAAGATGGCTATATTGTCCGC
    CAACAAGGAAAAGGAACCTTCGTTTCACGTGCACGCAAACACAAGCTTGTAGAGTTTTCA
    GATATTGAACTCTTCAATGCTAAAGATGATAAGGTAACTGTTCTTTCAATTGAACGCGGA
    AACAAGCTTGTTTACTTGGAAAAATTGGGTCTACGCGGCGATCAATTCTACTACAAGATT
    GAACGTATCCGCGAATCAAACGGTGTCGTTTATATCTACCATACATCCTACATCCCAGAA
    CAATATATCAACGCAAATTATCCAAACCTTGAATACTATAGTTCTATCTATAATCGTTTC
    AAGTTAGACTACCACATTCACATGAATGATGAACACTTTGAAGAAATCAACGAAATAGTC
    TTTCCAACTCCAGAACATGCGGCTTCAGTCCTCGGAGTCGATGAACAATTCCCTACTGTC
    CTACAAACTAAAATCACCAAACTCGAATCAACTGGCCAAGTTTTAGAATACAGTGAAACT
    TACAAACGATCTGATTACTACAAGATTAAATTTATCTCATGTGACCGTGATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    717

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HKM25_RS00415 [old locus tag: HKM25_84 ]
  • symbol: HKM25_RS00415
  • description: GntR family transcriptional regulator
  • length: 238
  • theoretical pI: 6.40701
  • theoretical MW: 27934.4
  • GRAVY: -0.522269

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 68.7)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 68.7)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 66.2)
    Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 63.3)
    and 6 more
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 50.7)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 50.7)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.6)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.6)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 18.6)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 16.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 60.7)
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 59.2)
    and 6 more
    Costars; Costars (PF14705; HMM-score: 18.4)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 15.1)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 14.6)
    MarR; MarR family (PF01047; HMM-score: 14.1)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.9)
    MarR_2; MarR family (PF12802; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by AgaR/CpsR*, TF important in N-acetylgalactosamine utilization: in TIGR4

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004463
    • TAT(Tat/SPI): 0.000186
    • LIPO(Sec/SPII): 0.000375
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001007202 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYKIKFISCDRDH

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Kaifeng Wu, Hongmei Xu, Yuqiang Zheng, Libin Wang, Xuemei Zhang, Yibing Yin
CpsR, a GntR family regulator, transcriptionally regulates capsular polysaccharide biosynthesis and governs bacterial virulence in Streptococcus pneumoniae.
Sci Rep: 2016, 6;29255
[PubMed:27386955] [WorldCat.org] [DOI] (I e)Muhammad Afzal, Sulman Shafeeq, Hifza Ahmed, Oscar P Kuipers
N-acetylgalatosamine-Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae.
Front Cell Infect Microbiol: 2016, 6;101
[PubMed:27672623] [WorldCat.org] [DOI] (I e)

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