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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-AUG-2013

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae gamPNI0373
  • locus tag: HMPREF1038_01061 [new locus tag: HMPREF1038_RS05230 ]
  • pan locus tag?: PNEUPAN002257000
  • symbol: HMPREF1038_01061
  • pan gene symbol?: pulA
  • synonym:
  • product: pullulanase, type I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: HMPREF1038_01061 [new locus tag: HMPREF1038_RS05230 ]
  • symbol: HMPREF1038_01061
  • product: pullulanase, type I
  • replicon: chromosome
  • strand: -
  • coordinates: 984423..986687
  • length: 2265
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCGCATTCATTACCATCGCAAGAATGGTGAATATGACACTTGCTCCTTTGTAAAAAGT
    CAGGATCAACGAATTGATTTGCTAACCTACAAAGAAGATTATTTTGGAGCTTTATTTAGC
    TTTGAACACCCAAGTTCACATGTTATAGAAAGCTTGAATTTTGTGGTTCATACAGGTCAA
    ACTAGTAAAGAATATTCTATCCGTTTTAATCACTATCCCCTATTAACAGAAGTCTGGATT
    TTGGAAGGCGATGATAGGATTTATTACTCTGAAAATCCTGCTATTGCCAGTCCTTTCTAT
    AAAAATCAAAATCCTTTTGCCTTTGATAAGGCCATTAACAGTGCTAGTTTTGACCATCAT
    TGGGGTTACCAAGGAGAATTGGGTTGCCGTGTAGAGGACAATCAGGCTCATTTTTCCCTC
    TGGTCACCTACAGCGACAGAAGTGCAAGTTGTCGTTTATGAATCAGCTGCTAATGATGCA
    CCCGTTTGGAAGACTTTTGAGATGAAAAGAGGCAATAGCTACTCTTATAATCATAAGGAC
    AATACAATCGGTGTCTGGAGTTTGGATGTTGAGGAAGATTTGGTAGGTAAGGCTTATCAG
    TATCAAGTCCAATTCCCTCATCACCAAACACTGACACGTGATCCTTATACAATCGCGACC
    AGCCCTGATGGCAAACGTTCAGCTATTCTGAGCCATGTAGAAAAGCAAGTTGAAAACTTC
    GAGGTTAAGCACGGTTCGGAGGCTACTTGGCGCTTGGAAAATCCATGTAAGGCAGTTATC
    TGTGAAATGCACATTCGTGATTTGACTAAATCACCTACATCGGGTGTAGATGAACATCTT
    CGAGGAACTTTCTTGGGTGCTGCTCAGGCTGGAACAGTTAACCAATACGGCCAGTCAACT
    GCTTTTGATTACATCAAGAAGCTGGGCTACAATTATGTTCAATTGCAACCAATTGCAGAC
    CGTCATAAAGAATACGATGAGGATGGAAATGTAACCTACAACTGGGGTTATGATCCACAA
    AACTATAACGCGCCAGAAACTAGTTTTTCAACTAATCCAGATGATCCAGCTCAGGTCATT
    CGTGATTTGAAGGTGATGGTTCAAGCTTATCACGATGCGGGTATTGGAGTCATTATGGAT
    GTAGTCTATAACCATACCTTCTCAGTTGTTGATGCACCATTCCAAACAACAGTCCCTGAT
    TACTATTATCGTATGAATCCAGATGGTACCTTCCAGAATGGAACGGGTGTTGGAAATGAA
    ACAGCCAGTGAACACGAAATGTTTCGCAAGTATATGATTGATTCTCTTCTATACTGGGTG
    CAGGAATATAATATTGACGGCTTCCGTTTTGACTTGATGGGGATTCATGATGTCAAGACC
    ATGCAGATGATTCGTCAAAGCTTGGATGAAATCGACTCCAACATTATCCTCTATGGAGAA
    GGATGGGATATGGGAACAGGTCTTGCCCCTTATGATAAGGCCAAGAAGGACAATGCCTAC
    CAGATGCCAAATATTGGTTTCTTTAATGACAATCAGCGCGATGCTGTCAAAGGGGGAGAA
    GTTTATGGTGCTATCAAGTCAGGTTTTGTCAGTGGTGCTGCGACAGAGCCAATTCTAGCT
    AAAGCAATCCTAGGAAGTCGTGAATTAGGAAGCTATACACATCCAAATCAGGTGCTTAAC
    TATGTAGAAGCCCATGACAATTACAATCTTCACGATTTATTGGCAACCCTTCATCCAGAC
    CAAAGTTCAGAGCAAATCATGCGCAAGGTCGAAACTGCCACAGCCATGAATCTGCTCATG
    CAGGGGATGGCCTTTATGGAAATCGGTCAAGAATTTGGTCGTACCAAACTGGTTGCGACT
    GGTGAAAATGGTGAGTTGACCCATGATGATAGAGAGCGTGCGATGAATAGCTATAATGCT
    CCTGACAGTGTGAACCAAGTGAACTGGAACTTGATTAATGAGCGTCAAGACAGTATTGAG
    TTTATCCGTCAAGTCATCCGATTGAAGACAAAAACTGGTGCCTTTTCTTACTCTAGCTAT
    GATGAAATTTACCATCATGTCTTTGTGCATTCGGCGATTGAACATAGCGGCTGCCTTATC
    TATGAAGTTCACGGCAAAGAACACCTCTTGGTGGTTGTGAATGCTAAATCAGAACCCTAT
    CAATTTGAAAATGCAGGAAATTTAGCTATGTTGGTAACCAACAGTCGCTCAAAAGAAGAT
    AATGTTTTAAATGATATTAGTCTAGCTGTCTTGAGTGTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2265

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: HMPREF1038_01061 [new locus tag: HMPREF1038_RS05230 ]
  • symbol: HMPREF1038_01061
  • description: pullulanase, type I
  • length: 754
  • theoretical pI: 5.02367
  • theoretical MW: 85817.8
  • GRAVY: -0.520557

⊟Function[edit | edit source]

  • TIGRFAM:
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 818.2)
    and 7 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 278.8)
    pullulanase, extracellular (TIGR02102; HMM-score: 272.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 162.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 73.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 62.4)
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 21.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 15.5)
  • TheSEED  :
    • Predicted glycogen debranching enzyme (pullulanase-like, but lacking signal peptide)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 51.4)
    GHD (CL0369) CBM_48; Carbohydrate-binding module 48 (Isoamylase N-terminal domain) (PF02922; HMM-score: 42.6)
    and 2 more
    Transthyretin (CL0287) PUD; Bacterial pullanase-associated domain (PF03714; HMM-score: 25.2)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004459
    • TAT(Tat/SPI): 0.000163
    • LIPO(Sec/SPII): 0.000663
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AFS43111 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYESAANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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