PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 04-FEB-2016

⊟Summary

• organism: Streptococcus pneumoniae INV104
• locus tag: INV104_19410 [new locus tag: INV104_RS11355 ]
• pan locus tag^?: PNEUPAN003949000
• symbol: htrA
• pan gene symbol^?: htrA
• synonym:
• product: serine protease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: INV104_19410 [new locus tag: INV104_RS11355 ]
• symbol: htrA
• product: serine protease
• replicon: chromosome
• strand: +
• coordinates: 2140098..2141279
• length: 1182
• essential: unknown

⊟Accession numbers

• Location: FQ312030 (2140098..2141279) NCBI
• BioCyc: see INV104_RS11355
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_2068 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGAAACATCTAAAAACATTTTACAAAAAATGGTTTCAATTATTAGTCGTTATCGTCATT
  AGCTTTTTTAGTGGAGCCTTGGGTAGTTTTTCAATAACTCAACTAACTCAAAAAAGTAGT
  GTAAACAACTCTAACAACAATAGTACTATTACACAAACTGCCTATAAGAACGAAAATTCA
  ACAACACAGGCTGTTAACAAAGTAAAAGATGCTGTTGTTTCTGTTATTACTTATTCGGCA
  AACAGACAAAATAGCGTATTTGGCAATGATGATACTGACACAGATTCTCAGCGAATCTCT
  AGTGAAGGATCTGGAGTTATTTATAAAAAGAATGATAAAGAAGCTTACATCGTCACCAAC
  AATCACGTTATAAATGGCGCCAGCAAAGTAGATATTCGATTGTCAGATGGGACTAAAGTA
  CCTGGAGAAATTGTCGGAGCTGACACTTTCTCTGATATTGCTGTCGTCAAAATCTCTTCA
  GAAAAAGTGACAACAGTAGCTGAGTTTGGTGATTCTAGTAAGTTAACTGTAGGAGAAACT
  GCTATTGCCATCGGTAGCCCGTTAGGTTCTGAATATGCAAATACTGTCACTCAAGGTATC
  GTATCCAGTCTCAATAGAAATGTATCCTTAAAATCGGAAGATGGACAAGCTATTTCTACA
  AAAGCCATCCAAACTGATACTGCTATTAACCCAGGTAACTCTGGCGGCCCACTGATCAAT
  ATTCAAGGGCAGGTTATCGGAATTACCTCAAGTAAAATTGCTACAAATGGAGGAACATCT
  GTAGAAGGTCTTGGTTTCGCAATTCCTGCAAATGATGCTATCAATATTATTGAACAGTTA
  GAAAAAAACGGAAAAGTGACGCGTCCAGCTTTGGGAATCCAGATGGTTAATTTATCTAAT
  GTGAGTACAAGCGACATCAGAAGACTCAATATTCCAAGTAATGTTACATCTGGTGTAATT
  GTTCGTTCGGTACAAAGTAATATGCCTGCCAATGGTCACCTTGAAAAATACGATGTAATT
  ACAAAAGTAGATGACAAAGAGATTGCTTCATCAACAGACTTACAAAGTGCTCTTTACAAC
  CATTCTATCGGAGACACCATTAAGATAACCTACTATCGTAACGGGAAAGAAGAAACTACC
  TCTATCAAACTTAACAAGAGTTCAGGTGATTTAGAATCTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1182
  

⊟Protein

⊟General

• locus tag: INV104_19410 [new locus tag: INV104_RS11355 ]
• symbol: HtrA
• description: serine protease
• length: 393
• theoretical pI: 6.97999
• theoretical MW: 41841.4
• GRAVY: -0.258015

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 275.9)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 275.9)
  and 4 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 178.6)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 178.6)
  Signal transduction Regulatory functions Other RNA chaperone Hfq (TIGR02383; HMM-score: 14.2)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 12.6)
• TheSEED  :
  • Serine protease Do-like HtrA (EC 3.4.21.107)
• PFAM:
  Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 105.4)
  PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 90.2)
  and 6 more
  Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 58.9)
  Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 27.7)
  PDZ-like (CL0466) PDZ; PDZ domain (PF00595; HMM-score: 21)
  Peptidase_PA (CL0124) Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 14.7)
  PDZ-like (CL0466) PDZ_6; PDZ domain (PF17820; HMM-score: 13.8)
  no clan defined FPP; Filament-like plant protein, long coiled-coil (PF05911; HMM-score: 9.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0006
  • Cytoplasmic Membrane Score: 0.8273
  • Cell wall & surface Score: 0.0108
  • Extracellular Score: 0.1613
• SignalP: Signal peptide SP(Sec/SPI) length 31 aa
  • SP(Sec/SPI): 0.773697
  • TAT(Tat/SPI): 0.010815
  • LIPO(Sec/SPII): 0.170382
  • Cleavage Site: CS pos: 31-32. SFS-IT. Pr: 0.1057
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq: CBW37622 NCBI
• UniProt:

⊟Protein sequence

• MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSEKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_2068 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Yasser Musa Ibrahim, Alison R Kerr, Jackie McCluskey, Tim J Mitchell
Role of HtrA in the virulence and competence of Streptococcus pneumoniae.
Infect Immun: 2004, 72(6);3584-91
[PubMed:15155668] [WorldCat.org] [DOI] (P p)Marco Cassone, Alyssa L Gagne, Lynn A Spruce, Steven H Seeholzer, Michael E Sebert
The HtrA protease from Streptococcus pneumoniae digests both denatured proteins and the competence-stimulating peptide.
J Biol Chem: 2012, 287(46);38449-59
[PubMed:23012372] [WorldCat.org] [DOI] (I p)Sacha F de Stoppelaar, Hester J Bootsma, Aldert Zomer, Joris J T H Roelofs, Peter W M Hermans, Cornelis van 't Veer, Tom van der Poll
Streptococcus pneumoniae serine protease HtrA, but not SFP or PrtA, is a major virulence factor in pneumonia.
PLoS One: 2013, 8(11);e80062
[PubMed:24244609] [WorldCat.org] [DOI] (I e)