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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 11-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae INV104
  • locus tag: INV104_RS04225 [old locus tag: INV104_07210 ]
  • pan locus tag?: PNEUPAN001824000
  • symbol: pbp3
  • pan gene symbol?: pbp3
  • synonym:
  • product: D-alanyl-D-alanine carboxypeptidase PBP3

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: INV104_RS04225 [old locus tag: INV104_07210 ]
  • symbol: pbp3
  • product: D-alanyl-D-alanine carboxypeptidase PBP3
  • replicon: chromosome
  • strand: -
  • coordinates: 803327..804568
  • length: 1242
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_017591 (803327..804568) NCBI
  • BioCyc: INV104_RS04225 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0767 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAAAAATATTTTTAACTTTGTTAACTGTCTCTCTTTTAGGGGGTGCTTCTACTGCT
    GTTGCTCAAGATTTTACCATTGCCGCTAAACATGCGATTGCTGTTGAGGCAAATACTGGT
    AAAATTCTCTATGAGAAGGATGCAACGCAACCTGTCGAAATTGCTTCCATAACAAAATTG
    ATTACTGCTTATCTGGTCTATGAAGCTTTGGAAAACGGAAGTATTACCCTCTCCACTCCT
    GTAGATATTTCTGATTATCCTTATCAATTGACGACAAATTCTGAAGCCAGTAATATTCCT
    ATGGAGGCCCGTAATTATACTGTCGAAGAGTTGCTTGAAGCAACTCTGGTATCTAGTGCC
    AACAGCGCCGCTATTGCCCTAGCTGAGAAAATTGCTGGCTCAGAAAAAGATTTCATCGAT
    ATGATGCGGGCAAAACTCTTGGAATGGGGAATTCAGGATGCCACTGTTGTCAATACGACA
    GGTCTTAACAATGAAACTCTAGGGGATAACATTTACCCAGGTTCTAAAAAAGATGAGGAA
    AATAAGCTTAGTGCTTATGATGTCGCTATCGTTGCTCGCAACCTCATCAAAAAATACCCA
    CAAGTCTTAGAAATCACCAAAAAACCTTCTTCTACTTTTGCTGGGATGACAATCACTTCA
    ACCAACTACATGTTAGAAGGTATGCCTGCTTACCGTGGTGGTTTTGATGGGCTAAAAACA
    GGAACAACAGATAAGGCTGGAGAGTCTTTTGTTGGTACTACTGTCGAAAAAGGCATGAGA
    GTCATCACAGTTGTTTTAAATGCAGATCATCAAGACAATAATCCTTACGCTCGATTTACA
    GCTACATCTTCCCTAATGGATTATATTTCTTCTACATTTACACTTCGCAAAATCGTTCAA
    CAAGGCGATGCCTATCAAGATAGCAAAGCCCCTGTACAAGATGGAAAAGAAGATACAGTA
    ACTGCAGTGGCTCCAGAGGATATCTATCTAATCGAACGTGTTGGGAATCAATCTTCCCAA
    TCTGTTCAATTCACACCTGATTCCAAAGCAATCCCAGCACCACTTGAAGCTGGAACAGTG
    GTTGGCCATTTGACTTATGAAGACAAGGACTTGATTGGTCAAGGTTACATCACCACAGAG
    CGCCCTAGTTTCGAAATGGTAGCAGACAAGAAAATTGAAAAAGCCTTCTTCTTAAAAGTT
    TGGTGGAATCAGTTTGTCCGCTTTGTTAACGAGAAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1242

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: INV104_RS04225 [old locus tag: INV104_07210 ]
  • symbol: Pbp3
  • description: D-alanyl-D-alanine carboxypeptidase PBP3
  • length: 413
  • theoretical pI: 4.60061
  • theoretical MW: 45165.8
  • GRAVY: -0.174334

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.16.4?  ExPASy
    Serine-type D-Ala-D-Ala carboxypeptidase Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
  • TIGRFAM:
  • TheSEED: see INV104_07210
  • PFAM:
    Beta-lactamase (CL0013) Peptidase_S11; D-alanyl-D-alanine carboxypeptidase (PF00768; HMM-score: 297.2)
    and 4 more
    no clan defined PBP5_C; Penicillin-binding protein 5, C-terminal domain (PF07943; HMM-score: 63.3)
    Beta-lactamase (CL0013) Beta-lactamase2; Beta-lactamase enzyme family (PF13354; HMM-score: 37.3)
    Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 25.5)
    Peptidase_S13; D-Ala-D-Ala carboxypeptidase 3 (S13) family (PF02113; HMM-score: 14.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0014
    • Cytoplasmic Membrane Score: 0.3212
    • Cell wall & surface Score: 0.0535
    • Extracellular Score: 0.6239
  • SignalP: Signal peptide SP(Sec/SPI) length 22 aa
    • SP(Sec/SPI): 0.981406
    • TAT(Tat/SPI): 0.000702
    • LIPO(Sec/SPII): 0.015671
    • Cleavage Site: CS pos: 22-23. AVA-QD. Pr: 0.8250
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000719149 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTTGLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERVGNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKIEKAFFLKVWWNQFVRFVNEKL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Cécile Morlot, Marjolaine Noirclerc-Savoye, André Zapun, Otto Dideberg, Thierry Vernet
The D,D-carboxypeptidase PBP3 organizes the division process of Streptococcus pneumoniae.
Mol Microbiol: 2004, 51(6);1641-8
[PubMed:15009891] [WorldCat.org] [DOI] (P p)Mohammed R Abdullah, Javier Gutiérrez-Fernández, Thomas Pribyl, Nicolas Gisch, Malek Saleh, Manfred Rohde, Lothar Petruschka, Gerhard Burchhardt, Dominik Schwudke, Juan A Hermoso, Sven Hammerschmidt
Structure of the pneumococcal l,d-carboxypeptidase DacB and pathophysiological effects of disabled cell wall hydrolases DacA and DacB.
Mol Microbiol: 2014, 93(6);1183-206
[PubMed:25060741] [WorldCat.org] [DOI] (I p)

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