From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 70585
  • locus tag: SP70585_2130 [new locus tag: SP70585_RS10580 ]
  • pan locus tag?: PNEUPAN003614000
  • symbol: ackA
  • pan gene symbol?: ackA
  • synonym:
  • product: acetate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP70585_2130 [new locus tag: SP70585_RS10580 ]
  • symbol: ackA
  • product: acetate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1952676..1953866
  • length: 1191
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACAAAAACAATTGCAATCAATGCAGGAAGTTCAAGTTTGAAATGGCAATTATACTTA
    ATGCCAGAAGAAAAAGTATTGGCGAAAGGTTTGATTGAACGTATCGGTTTGAAAGATTCA
    ATTTCAACTGTAAAATTTGACGGCCGTTCTGAACAACAAATTTTGGATATCGAAAATCAT
    ACACAAGCTGTTAAAATTTTATTGGATGACTTGATTCGTTTCGATATTATCAAGGCTTAT
    GACGAGATTACAGGTGTTGGCCACCGTGTTGTAGCAGGTGGAGAATATTTCAAAGAATCA
    ACAGTTGTTGAGGGAGATGTTTTAGAAAAAGTTGAAGAGTTGAGTTTGTTGGCTCCTCTA
    CACAACCCGGCCAATGCAGCAGGTGTTCGTGCCTTCAAGGAATTGTTGCCAGACATTACC
    AGTGTAGTTGTTTTTGATACTTCCTTCCACACAAGTATGCCAGAGAAAGCTTATCGCTAC
    CCTCTACCAACAAAATATTACACAGAAAACAAGGTTCGTAAATACGGTGCTCATGGTACA
    AGTCACCAGTTTGTAGCAGGAGAAGCTGCAAAACTCTTGGGACGTCCATTAGAAGACTTG
    AAGTTAATTACCTGTCATATTGGTAACGGAGGCTCAATTACAGCTGTGAAAGCCGGCAAA
    TCTGTAGACACTTCTATGGGGTTCACTCCTCTTGGTGGTATTATGATGGGAACGCGTACA
    GGGGATATTGATCCAGCTATCATTCCTTATTTAATGCAATATACAGAGGATTTTAACACA
    CCAGAAGATATCAGTCGTGTTCTTAACCGTGAATCAGGTCTTTTGGGAGTTTCTGCTAAT
    TCTAGCGATATGCGCGATATAGAAGCAGCTGTAGCAGAAGGGAATCACGAGGCTAGCTTG
    GCTTATGAAATGTATGTTGACCGTATCCAAAAATATATCGGTCAGTACCTTGCAGTGCTA
    AATGGAGCAGATGCCATTGTTTTCACAGCAGGTGTCGGTGAAAATGCAGAGAATTTCCGT
    CGTGATGTAATCTCAGGGATTTCGTGGTTTGGTTGTGATGTTGATGATGAAAAGAATGTC
    TTTGGCGTTACAGGAGACATCTCAACAGAGGCAGCTAAAATCCGTGTCTTGGTTATTCCA
    ACAGATGAAGAATTAGTCATTGCCCGTGACGTTGAACGCTTGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1191

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP70585_2130 [new locus tag: SP70585_RS10580 ]
  • symbol: AckA
  • description: acetate kinase
  • length: 396
  • theoretical pI: 4.80468
  • theoretical MW: 43383.1
  • GRAVY: -0.114646

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.1?  ExPASy
    Acetate kinase ATP + acetate = ADP + acetyl phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Fermentation acetate kinase (TIGR00016; EC 2.7.2.1; HMM-score: 509.6)
    and 2 more
    Metabolism Energy metabolism Fermentation butyrate kinase (TIGR02707; EC 2.7.2.7; HMM-score: 66.9)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein AD (TIGR02845; HMM-score: 10.8)
  • TheSEED  :
    • Acetate kinase (EC 2.7.2.1)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  Acetate kinase (EC 2.7.2.1)
    and 2 more
    Carbohydrates Fermentation Fermentations: Lactate  Acetate kinase (EC 2.7.2.1)
    Carbohydrates Fermentation Fermentations: Mixed acid  Acetate kinase (EC 2.7.2.1)
  • PFAM:
    Actin_ATPase (CL0108) Acetate_kinase; Acetokinase family (PF00871; HMM-score: 503)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004482
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.000285
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYPLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGENAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Smirla Ramos-Montañez, Krystyna M Kazmierczak, Kristy L Hentchel, Malcolm E Winkler
Instability of ackA (acetate kinase) mutations and their effects on acetyl phosphate and ATP amounts in Streptococcus pneumoniae D39.
J Bacteriol: 2010, 192(24);6390-400
[PubMed:20952579] [WorldCat.org] [DOI] (I p)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP70585_2130&oldid=31335"