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serotype 1

NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_0701 [new locus tag: SPAP_RS03485 ]
  • pan locus tag?: PNEUPAN001647000
  • symbol: SPAP_0701
  • pan gene symbol?: thiE-2
  • synonym: thiE
  • product: Thiamine monophosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_0701 [new locus tag: SPAP_RS03485 ]
  • symbol: SPAP_0701
  • product: Thiamine monophosphate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 672124..672756
  • length: 633
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAATAGAGAAGCACTTAGACTATATCTGGTAACCAATCGCTATCAAGATTCCGTGGAA
    AGCTTTCTTGCAAAAATTGAGACGGCCTGCCGTTCAGGGGTTACCATAGTCCAATTGCGA
    GAAAAAAATCTCACAACCAATCAATATTATCAACTGGCAAAACAAGTCAAGGAAATAACA
    GATGCCTATCAGGTCCCCTTGATAATCGATGATCGGTTGGATGTTTGTCTTGCGGTTGAT
    GCAGCAGGTCTGCATATCGGAGATGATGAATTACCAGTTTCGGTAGCTCGCAAAGTCTTG
    GGTCCTGAAAAAATCCTCGGTGTCACCGCTAAAACGGTTAAAAGAGCTCTGGAAGCGGAG
    AAATCAGGTGCAGATTACTTGGGTACAGGAGCCATTTTCCCAACCACCACCAAGGAAAAT
    GCGCCCATTACTCTGATATCGACCTTGAAAACAATTTGCCAAACGGTTGCCATTCCAGTA
    GTTGCTATTGGAGGCTTGACTTCGGAGAATATTGATCAGCTTATGGGCACTGGCATAGCT
    GGTGTAGCTGTTGTACGTGATTTGATGCAAGCAGAAGATATTGAGGCAAAAACGCAAGCC
    TTTTTAACAAAGTTGCATGACATTCTTTCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_0701 [new locus tag: SPAP_RS03485 ]
  • symbol: SPAP_0701
  • description: Thiamine monophosphate synthase
  • length: 210
  • theoretical pI: 5.07891
  • theoretical MW: 22758.1
  • GRAVY: 0.100476

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 224.2)
    and 5 more
    predicted phospho-2-dehydro-3-deoxyheptonate aldolase (TIGR01949; EC 4.2.1.-; HMM-score: 17.5)
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 16.5)
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 15.9)
    Unknown function General IMP dehydrogenase family protein (TIGR01304; HMM-score: 13.8)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 12.5)
  • TheSEED  :
    • Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)
  • PFAM:
    TIM_barrel (CL0036) TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 210.9)
    and 7 more
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 26)
    PK_TIM (CL0151) PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 17)
    PELOTA (CL0101) RNase_P_pop3; RNase P subunit Pop3 (PF08228; HMM-score: 15.6)
    TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 14.2)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 13.7)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 11.9)
    no clan defined EMP70; Endomembrane protein 70 (PF02990; HMM-score: 10.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.998
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0012
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006466
    • TAT(Tat/SPI): 0.001112
    • LIPO(Sec/SPII): 0.008396
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM84294 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNREALRLYLVTNRYQDSVESFLAKIETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPVVAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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