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serotype 1

NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_1675 [new locus tag: SPAP_RS08290 ]
  • pan locus tag?: PNEUPAN003086000
  • symbol: SPAP_1675
  • pan gene symbol?: murF
  • synonym:
  • product: UDP-N-acetylmuramyl pentapeptide synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_1675 [new locus tag: SPAP_RS08290 ]
  • symbol: SPAP_1675
  • product: UDP-N-acetylmuramyl pentapeptide synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1578086..1579459
  • length: 1374
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTAACAATCCATGAAATTGCCCAAGTTGTTGGAGCCAAAAATGATATCAGTATC
    TTTGAGGACACCCAGTTAGAAAAAGCTGAGTTTGATAGTCGTTTGATTGGAACTGGAGAT
    TTATTTGTGCCACTTAAAGGTGCGCGTGATGGCCATGACTTTATTGAAACAGCCTTTGAA
    AATGGTGCAGCAGTAACCTTGTCTGAGAAAGAGGTCTCAAATCATCCTTACATTCTAGTA
    GATGATGTTTTGACAGCCTTTCAATCCCTAGCATCCTACTATCTTGAAAAAACGACTGTT
    GATGTCTTTGCTGTTACAGGTTCAAATGGCAAGACAACGACTAAGGATATGTTGGCGTAT
    TTACTGTCAACAAGATACAAGACCTACAAAACACAAGGCAATTACAATAATGAGATTGGC
    CTTCCTTACACAGTTCTCCACATGCCTGAAGGAACAGAAAAGTTGGTCTTGGAGATGGGG
    CAGGACCACTTGGGAGATATTCATCTCTTGTCTGAATTGGCTCGTCCAAAAACAGCCATC
    GTGACCTTGGTTGGAGAAGCTCATTTGGCCTTTTTCAAAGATCGTTCTGAGATTGCTAAA
    GGAAAAATGCAAATTGCAGACGGAATGGCTTCAGGTTCCTTGCTTTTAGCGCCGGCTGAC
    CCTATCGTAGAGGACTATTTGCCAACTGATAAAAAGGTGGTTCGTTTTGGTCAAGGAGCA
    GAGCTGGAAATTACTGACTTGGTTGAGCGCAAAGATAGTCTGACCTTCAAGGCCAATTTC
    TTAGAGCAAGTCCTTGATTTGCCAGTAACTGGCAAGTATAATGCGACAAATGCTATGATT
    GCATCCTATGTTGCCTTGCAAGAAGGAGTTTCAGAGGAGCAAATTCATCAGGCCTTCCAA
    GATCTTGAATTGACGCGTAACCGTACCGAATGGAAGAAAGCAGCCAATGGAGCAGATATC
    CTGTCTGATGTATACAATGCCAATCCAACTGCTATGAAGTTGATTTTAGAGACTTTCTCT
    GCCATTCCAGCCAATGAAGGTGGCAAGAAAATTGCGGTTTTAGCGGACATGAAGGAACTG
    GGTAACCAGTCAGTTCAACTTCATAATCAGATGATTTTGAGCCTTTCTCCAGATGTGCTT
    GATACCGTGATTTTCTATGGAGAAGACATTGCTGAATTAGCCCAATTGGCCAGTCAAATG
    TTCCCAATTGGCCACGTTTACTACTTCAAGAAAACAGAAGACCAAGACCAATTTGAAGAC
    CTAGTCAAGCAGGTCAAGGAAAGCCTCAGTGCCAATGATCAAATCTTGCTCAAGGGCTCT
    AACTCTATGAACCTAGCTATGTTGGTAGAAAGTTTAGAAAATGAAACCAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1374

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_1675 [new locus tag: SPAP_RS08290 ]
  • symbol: SPAP_1675
  • description: UDP-N-acetylmuramyl pentapeptide synthase
  • length: 457
  • theoretical pI: 4.4922
  • theoretical MW: 50502
  • GRAVY: -0.17593

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 356.4)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 89.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 79.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 66.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 32.8)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 29.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 20.2)
  • TheSEED  :
    • UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan biosynthesis--gjo  UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)
  • PFAM:
    P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 130)
    and 2 more
    no clan defined Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 39.8)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 22.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9992
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0003
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00363
    • TAT(Tat/SPI): 0.000193
    • LIPO(Sec/SPII): 0.000263
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM85259 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAYLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAPADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNATNAMIASYVALQEGVSEEQIHQAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAELAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVESLENETK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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