From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_2186 [new locus tag: SPAP_RS10815 ]
  • pan locus tag?: PNEUPAN003823000
  • symbol: SPAP_2186
  • pan gene symbol?: cbpN
  • synonym: pcpA
  • product: Choline binding protein PcpA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_2186 [new locus tag: SPAP_RS10815 ]
  • symbol: SPAP_2186
  • product: Choline binding protein PcpA
  • replicon: chromosome
  • strand: -
  • coordinates: 2022694..2024259
  • length: 1566
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAAAAAACTACAATATTATCATTAACTACAGCTGCAGTTATTTTAGCAGCATATGTC
    CCTAATGAACCAATCCTAGCAGATACTCCTAGTTCGGAAGTAATCAAAGAGACTAAAGTT
    GGAAGTATTATTCAACAAAATAATATCAAATATAAGGTTCTAACTGTAGAAGGTAACATA
    GGAACTGTTCAAGTGGGTAATGGAGTTACTCCTGTAGAGTTTGAAGCTGGTCAAGATGGA
    AAACCATTCACGATTCCTACAAAAATCACAGTAGGTGATAAAGTATTTACCGTTACTGAA
    GTAGCTAGTCAAGCTTTTAGTTATTATCCAGATGAAACAGGTAGAATTGTCTACTATCCT
    AGCTCTATTACTATCCCATCAAGCATAAAAAAAATACAAAAAAAAGGCTTCCATGGAAGT
    AAAGCTAAAACTATTATTTTTGACAAAGGTAGTCAGCTGGAGAAAATTGAAGATAGAGCT
    TTTGATTTTTCTGAATTAGAAGAGATTGAATTGCCTGCATCTCTAGAATATATTGGAACA
    AGTGCATTTTCTTTTAGTCAAAAATTGAAAAAGCTAACCTTTTCCTCAAGTTCAAAATTA
    GAATTAATATCACATGAGGCTTTTGCTAATTTATCAAATTTAGAGAAACTAACATTACCA
    AAATCGGTTAAAACATTAGGAAGTAATCTATTTAGACTCACTACTAGCTTAAAACATGTT
    GATGTTGAAGAAGGAAATGAATCGTTTGCCTCAGTTGATGGTGTTTTGTTTTCAAAAGAT
    AAAACCCAATTAATTTATTATCCAAGTCAAAAAAATGACGAAAGTTATAAAACGCCTAAG
    GGGACAAAAGAACTTGCATCATATTCGTTTAATAAAAATTCTTACTTGAAAAAACTCGAA
    TTGAATGAAGGTTTAGAAAAAATTGGTACTTTTGCATTTGCAGATGCGATTAAACTTGAA
    GAAATTAGCTTACCAAATAGTTTAGAAACTATTGAACGTTTAGCCTTTTACGGTAATTTA
    GAATTAAAAGAACTTATATTACCAGATAATGTTAAAAATTTTGGTAAACACGTTATGAAC
    GGTTTACCAAAATTAAAAAGTTTAACAATTGGTAATAATATCAACTCATTGCCGTCCTTC
    TTCCTAAGTGGCGTCTTAGATTCATTAAAGGAAATTCATATTAAGAATAAAAGTACAGAG
    TTTTCTGTGAAAAAAGATACATTTGCAATTCCTGAAACTGTTAAGTTCTATGTAACATCA
    GAACATATAAAAGATGTTCTTAAATCAAATTTATCTACTAGTAATGATATCATTGTTGAA
    AAAGTAGATAATATAAAACAAGAAACTGATGTAGCTAAACCTAAAAAGAATTCTAATCAG
    GGAGTAGTTGGTTGGGTTAAAGACAAAGGTTTATGGTATTACTTAAACGAATCAGGTTCA
    ATGGCTACTGGTTGGTTTACAGTTTCTGGTAAATGGTACTATACCTATAATTCAGGAGAT
    TTATTAGTAAACACGACTACACCCGATGGCTATCGAGTCAATGCTAACGGTGAGTGGGTA
    GGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1566

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_2186 [new locus tag: SPAP_RS10815 ]
  • symbol: SPAP_2186
  • description: Choline binding protein PcpA
  • length: 521
  • theoretical pI: 7.80275
  • theoretical MW: 57775.3
  • GRAVY: -0.299616

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 14.9)
  • TheSEED  :
    • Choline binding protein PcpA
  • PFAM:
    LRR (CL0022) LRR_5; BspA type Leucine rich repeat region (6 copies) (PF13306; HMM-score: 193.5)
    and 5 more
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 41.7)
    LRR (CL0022) LRR_8; Leucine Rich Repeat (PF13855; HMM-score: 38.6)
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 38.1)
    Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 32.1)
    LRR (CL0022) LRR_4; Leucine Rich repeats (2 copies) (PF12799; HMM-score: 15.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.0037
    • Cell wall & surface Score: 0.045
    • Extracellular Score: 0.9512
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.97212
    • TAT(Tat/SPI): 0.003655
    • LIPO(Sec/SPII): 0.015784
    • Cleavage Site: CS pos: 27-28. ILA-DT. Pr: 0.9403
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM85717 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKGTKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Laura Selva, Pilar Ciruela, Krystle Blanchette, Eva del Amo, Roman Pallares, Carlos J Orihuela, Carmen Muñoz-Almagro
Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae.
PLoS One: 2012, 7(7);e41587
[PubMed:22848535] [WorldCat.org] [DOI] (I p)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPAP_2186&oldid=44376"