PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 25-OCT-2019

⊟Summary

• organism: Streptococcus pneumoniae ATCC 49619
• locus tag: SPAT_0508 [new locus tag: EL263_RS02750 ]
• pan locus tag^?: PNEUPAN001429000
• symbol: trmB
• pan gene symbol^?: trmB
• synonym:
• product: tRNA (guanine-N(7)-)-methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAT_0508 [new locus tag: EL263_RS02750 ]
• symbol: trmB
• product: tRNA (guanine-N(7)-)-methyltransferase
• replicon: chromosome
• strand: +
• coordinates: 504226..504861
• length: 636
• essential: unknown

⊟Accession numbers

• Location: AP018938 (504226..504861) NCBI
• BioCyc: see EL263_RS02750
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0477 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAGAGTTAGAAATCGTAAAGGGGCGACAGAATTACTAGAGGCAAATCCCCAGTATGTG
  GTCCTCAATCCTTTGGAAGCCAAGGGAAAATGGCGGGACTTGTTTGGAAATGATAATCCC
  ATTCATGTGGAAGTTGGAAGTGGAAAGGGTGCCTTTGTTTCAGGTATGGCAAAGCAGAAC
  CCTGACATCAACTATATCGGGATTGATATTCAAAAGTCTGTTTTGAGCTACGCTTTGGAC
  AAGGTGCTTGAAGTTGGAGTGCCTAACATTAAGCTCTTGTGGGTAGATGGTTCTGACTTG
  ACTGACTACTTTGAAGACGGTGAGATTGATCGTTTGTATCTGAACTTTTCAGATCCTTGG
  CCGAAAAAACGCCATGAAAAGCGTCGTTTGACCTATAAGACCTTCTTGGATACCTTCAAG
  CGTATCTTACCTGAAAATGGTGAAATTCATTTCAAGACGGATAACCGTGGCTTGTTTGAG
  TACAGCCTAGTGAGCTTTTCTCAGTATGGCATGAAGCTCAATGGTGTCTGGCTTGATTTA
  CATGCCAGTGGTTTTGAAGGCAATGTCATGACAGAATACGAGCAAAAATTCTCCAACAAG
  GGGCAAGTTATCTACCGAGTTGAGGCAGAATTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  636
  

⊟Protein

⊟General

• locus tag: SPAT_0508 [new locus tag: EL263_RS02750 ]
• symbol: TrmB
• description: tRNA (guanine-N(7)-)-methyltransferase
• length: 211
• theoretical pI: 6.53471
• theoretical MW: 24306.4
• GRAVY: -0.503791

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 191.9)
  and 4 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 15.3)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 14.8)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 12)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 11.2)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 158.9)
  and 8 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.4)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 27.2)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 24.7)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 21.3)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 17.5)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.5)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 12.1)
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9932
  • Cytoplasmic Membrane Score: 0.0006
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0062
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010383
  • TAT(Tat/SPI): 0.000764
  • LIPO(Sec/SPII): 0.000774
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBG38565 NCBI
• UniProt:

⊟Protein sequence

• MRVRNRKGATELLEANPQYVVLNPLEAKGKWRDLFGNDNPIHVEVGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSLVSFSQYGMKLNGVWLDLHASGFEGNVMTEYEQKFSNKGQVIYRVEAEF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0477 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications