PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 25-OCT-2019

⊟Summary

• organism: Streptococcus pneumoniae ATCC 49619
• locus tag: SPAT_2019 [new locus tag: EL263_RS10665 ]
• pan locus tag^?: PNEUPAN003677000
• symbol: tkt_3
• pan gene symbol^?: tktN
• synonym:
• product: Transketolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAT_2019 [new locus tag: EL263_RS10665 ]
• symbol: tkt_3
• product: Transketolase
• replicon: chromosome
• strand: -
• coordinates: 1971638..1972495
• length: 858
• essential: unknown

⊟Accession numbers

• Location: AP018938 (1971638..1972495) NCBI
• BioCyc: see EL263_RS10665
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1958 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGATTTTAAGTGAAAATAGAGAAGATGAGTTAAGAAAATTTGCGACTAACATCCGATTA
  AATACTCTTAGAACATTGAATCATCTTGGATTCGGACATTACGGAGGAAGTCTGTCTATC
  GTAGAAGTTTTAGCAGTGCTTTATGGTGAAATAATGCCAATGACTCCAGAAATATTTGCA
  GCACGAGATAGAGATTATTTCATATTATCAAAAGGTCATGGAGGACCAGCTCTATACAGT
  ACACTCTATTTGAATGGTTTCTTTGACAAAGAATTCTTATATTCTTTAAATACAAATGGA
  ACCAAATTACCGTCTCATCCTGATAGAAATCTAACGCCAGGCATAGATATGACAACGGGC
  TCTTTAGGACAAGGAATTAGTGTTGCAACTGGACTTGCATATGGTCAGAGAATAAGAAAG
  AGTCCCTTTTATACTTATGCTATTGTTGGAGATGGTGAGTTAAATGAGGGACAATGTTGG
  GAGGCTATACAGTTTGCTTCTCATCAACAGTTATCCAACTTAATTGTATTTGTTGATGAT
  AACAAAAAACAATTAGATGGTTTTACAAAGGATATTTGTAATTCAGGTGATTTCGTAGAA
  AAATTTTCAGCATTTGGATTTGAATCCATTAGGGTCAAGGGTTCAGATATTAGAGAAATT
  TATGAAGGGATTGTCCAATTAAAACAGTCAAATAATTCATCACCTAAGTGCATTGTATTA
  GATACTATTAAAGGTCAAGGGGTTCAAGAGCTGGAAGAAATGAAATCCAATCATCATCTT
  CGCCCTACTGTAGAGGAGAAACAAATGTTAACTTCAGTTGTAGAAAGATTAAGTCAGGAA
  TTGGAGGAAACAGAATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  858
  

⊟Protein

⊟General

• locus tag: SPAT_2019 [new locus tag: EL263_RS10665 ]
• symbol: Tkt_3
• description: Transketolase
• length: 285
• theoretical pI: 5.0686
• theoretical MW: 31945.9
• GRAVY: -0.334737

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Pentose phosphate pathway transketolase (TIGR00232; EC 2.2.1.1; HMM-score: 163.2)
  and 8 more
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 54)
  pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 46.7)
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 41.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 39)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 39)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 39)
  alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 38.9)
  sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 11.9)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 158.3)
  and 4 more
  DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 47.1)
  E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 33.6)
  XFP_N; XFP N-terminal domain (PF09364; HMM-score: 24.3)
  TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 20.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007708
  • TAT(Tat/SPI): 0.005932
  • LIPO(Sec/SPII): 0.001462
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBG40022 NCBI
• UniProt:

⊟Protein sequence

• MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCWEAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNSGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1958 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications