PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 30-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae CGSP14
• locus tag: SPCG_0617 [new locus tag: SPCG_RS03245 ]
• pan locus tag^?: PNEUPAN001603000
• symbol: SPCG_0617
• pan gene symbol^?: yesN
• synonym:
• product: DNA-binding response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPCG_0617 [new locus tag: SPCG_RS03245 ]
• symbol: SPCG_0617
• product: DNA-binding response regulator
• replicon: chromosome
• strand: +
• coordinates: 620400..621155
• length: 756
• essential: unknown

⊟Accession numbers

• Location: CP001033 (620400..621155) NCBI
• BioCyc: see SPCG_RS03245
• MicrobesOnline: 5759945 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_0574 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  GTGGTAAAGGAGCAGATCATGACCTACACAATCTTAATCGTAGAAGATGAATATCTGGTA
  AGACAAGGTTTGACTAAACTGGTCAATGTAGCAGCCTACGATATGGAAATCATCGGTCAG
  GCTGAAAATGGAAGGCAGGCTTGGGAATTGATCCAAAAGCAGGTGCCAGATATCATTTTA
  ACCGATATCAACATGCCTCATCTAAATGGCATCCAGTTGGCCAGTCTGGTACGAGAAACC
  TATCCTCAGGTTCATTTGGTCTTTTTAACAGGTTACGATGATTTTGATTATGCCTTGTCT
  GCTGTCAAACTAGGTGTGGACGACTACCTGCTCAAACCCTTTTCTCGTCAGGATATTGAG
  GAAATGTTGGGGAAAATCAAACAAAAACTAGACAAGGAAGAGAAAGAAGAGCAGTTACAA
  GATTTATTGACTAATAGGTTTGAAGGAAACATGGCCCAGAAAATCCAGTCTCATCTGGCT
  GATAGCCAATTTAGTTTAAAGTCTTTAGCCAGTGACTTAGGTTTTAGTCCGACCTATCTG
  AGTTCCTTGATTAAGAAAGAGTTGGGCTTGCCTTTTCAGGATTATCTGGTGAGAGAACGT
  GTTAAACAAGCCAAGCTCTTGCTTTTAACTACAGATCTGAAGATTTATGAGATCGCAGAG
  AAGGTTGGTTTTGAAGATATGAACTATTTTACCCAACGTTTTAAGCAGATTGCAGGTGTG
  ACACCTCGTCAGTTTAAGAAGGGAGAAGACCGATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  756
  

⊟Protein

⊟General

• locus tag: SPCG_0617 [new locus tag: SPCG_RS03245 ]
• symbol: SPCG_0617
• description: DNA-binding response regulator
• length: 251
• theoretical pI: 4.93917
• theoretical MW: 28991.2
• GRAVY: -0.305179

⊟Function

• TIGRFAM:
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 65.6)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 65.6)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 65.6)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 64.8)
  YSIRK-targeted surface antigen transcriptional regulator (TIGR04094; HMM-score: 61.7)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 61.1)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 61.1)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 58.7)
  and 4 more
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 44.6)
  4-hydroxyphenylacetate catabolism regulatory protein HpaA (TIGR02297; HMM-score: 40.6)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 34.4)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 33.2)
• TheSEED  :
  • Two-component response regulator YesN, associated with MetSO reductase
  Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Two-component response regulator yesN, associated with MetSO reductase
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 103.3)
  and 5 more
  HTH (CL0123) HTH_18; Helix-turn-helix domain (PF12833; HMM-score: 69.8)
  HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 60.6)
  ArsR; ArsR transcriptional regulator (PF09824; HMM-score: 16.1)
  MazG (CL0231) MazG-like; MazG-like family (PF12643; HMM-score: 14)
  no clan defined DUF5678; Family of unknown function (DUF5678) (PF18929; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007755
  • TAT(Tat/SPI): 0.000502
  • LIPO(Sec/SPII): 0.00048
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ACB89869 NCBI
• UniProt: B2IMX3 UniProt

⊟Protein sequence

• MVKEQIMTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWELIQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSLKSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGFEDMNYFTQRFKQIAGVTPRQFKKGEDR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPCG_0617 > SPCG_0618

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0574 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Clare E Blue, Tim J Mitchell
Contribution of a response regulator to the virulence of Streptococcus pneumoniae is strain dependent.
Infect Immun: 2003, 71(8);4405-13
[PubMed:12874319] [WorldCat.org] [DOI] (P p)Wouter T Hendriksen, Nuno Silva, Hester J Bootsma, Clare E Blue, Gavin K Paterson, Alison R Kerr, Anne de Jong, Oscar P Kuipers, Peter W M Hermans, Tim J Mitchell
Regulation of gene expression in Streptococcus pneumoniae by response regulator 09 is strain dependent.
J Bacteriol: 2007, 189(4);1382-9
[PubMed:17085554] [WorldCat.org] [DOI] (P p)Stephanie Hirschmann, Alejandro Gómez-Mejia, Ulrike Mäder, Julia Karsunke, Dominik Driesch, Manfred Rohde, Susanne Häussler, Gerhard Burchhardt, Sven Hammerschmidt
The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.
Microorganisms: 2021, 9(3);
[PubMed:33668344] [WorldCat.org] [DOI] (P e)Stephanie Hirschmann, Alejandro Gómez-Mejia, Thomas P Kohler, Franziska Voß, Manfred Rohde, Max Brendel, Sven Hammerschmidt
The Two-Component System 09 of Streptococcus pneumoniae Is Important for Metabolic Fitness and Resistance during Dissemination in the Host.
Microorganisms: 2021, 9(7);
[PubMed:34201716] [WorldCat.org] [DOI] (P e)