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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae CGSP14
  • locus tag: SPCG_0676 [new locus tag: SPCG_RS03545 ]
  • pan locus tag?: PNEUPAN001649000
  • symbol: copY
  • pan gene symbol?: copY
  • synonym:
  • product: transcriptional repressor, putative

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPCG_0676 [new locus tag: SPCG_RS03545 ]
  • symbol: copY
  • product: transcriptional repressor, putative
  • replicon: chromosome
  • strand: +
  • coordinates: 678532..678957
  • length: 426
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGACAAATGTAGATTTTGGAGGTATGGTAATGCAGATTTCAGATGCAGAATGGCAGGTC
    ATGAAGATTATTTGGATGCAGGGGGAGCAGACCAGTACAGATTTGATTAGGGTTTTGGCA
    GAGCGGTTTGACTGGTCCAAGTCCACGGTTCAGACACTTTTGGCTCGTCTGGTTGAGAAA
    GAGTGTTTGACTCGGAAAAAAGAAGGCAAGTCCTTTGTTTATTCAGCCCTTTTAACTCTA
    GACCAAAGTCGGGATTTACTTGTCCAAGATATCAAAGACAAGGTTTGTTCCCGTAGGATT
    AGGAATTTGTTGGCTGACTTGATTGTAGAATGTGAATTTACTCAGACTGACTTGGAAGAC
    TTGGAAGCTGTGATTTCAGAGAAGAAATCAAGTGCTGTAACAGAAGTAAGATGTAATTGT
    ATGTAA
    60
    120
    180
    240
    300
    360
    420
    426

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPCG_0676 [new locus tag: SPCG_RS03545 ]
  • symbol: CopY
  • description: transcriptional repressor, putative
  • length: 141
  • theoretical pI: 4.6982
  • theoretical MW: 16200.7
  • GRAVY: -0.147518

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 181.2)
    Signal transduction Regulatory functions DNA interactions copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 181.2)
    and 5 more
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 19.6)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 19.6)
    antiviral radical SAM protein viperin (TIGR04278; HMM-score: 16)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 15.5)
    Signal transduction Regulatory functions DNA interactions phenylacetic acid degradation operon negative regulatory protein PaaX (TIGR02277; HMM-score: 12.5)
  • TheSEED  :
    • Negative transcriptional regulator-copper transport operon
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Copper homeostasis  Negative transcriptional regulator-copper transport operon
  • PFAM:
    HTH (CL0123) Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 114.2)
    and 15 more
    MarR; MarR family (PF01047; HMM-score: 29.7)
    MarR_2; MarR family (PF12802; HMM-score: 28)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 18.7)
    PaaX; PaaX-like protein (PF07848; HMM-score: 18.7)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 17.3)
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 16.9)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 16.7)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 16.1)
    ANAPC2; Anaphase promoting complex (APC) subunit 2 (PF08672; HMM-score: 14.5)
    TBPIP; TBPIP/Hop2 winged helix domain (PF07106; HMM-score: 14.1)
    no clan defined GPP34; Golgi phosphoprotein 3 (GPP34) (PF05719; HMM-score: 13.6)
    HTH (CL0123) SMC_ScpB; Segregation and condensation complex subunit ScpB (PF04079; HMM-score: 13.3)
    Cullin_Nedd8; Cullin protein neddylation domain (PF10557; HMM-score: 13.2)
    Ferritin (CL0044) DUF2202; Uncharacterized protein domain (DUF2202) (PF09968; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Copper ion, (Cu2+)
  • genes regulated by CopY, TF important in Copper homeostasisRegPrecise
    repression
    copY > cupA* > ctpA
    transcription unit transferred from TIGR4 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9778
    • Cytoplasmic Membrane Score: 0.0104
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0117
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002148
    • TAT(Tat/SPI): 0.000153
    • LIPO(Sec/SPII): 0.000282
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACB89928 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTNVDFGGMVMQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLLARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECEFTQTDLEDLEAVISEKKSSAVTEVRCNCM

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CopY (repression) regulon
    CopY(TF)important in Copper homeostasis; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Sulman Shafeeq, Hasan Yesilkaya, Tomas G Kloosterman, Geetha Narayanan, Michal Wandel, Peter W Andrew, Oscar P Kuipers, Julie A Morrissey
The cop operon is required for copper homeostasis and contributes to virulence in Streptococcus pneumoniae.
Mol Microbiol: 2011, 81(5);1255-70
[PubMed:21736642] [WorldCat.org] [DOI] (I p)Miranda J Neubert, Elizabeth A Dahlmann, Andrew Ambrose, Michael D L Johnson
Copper Chaperone CupA and Zinc Control CopY Regulation of the Pneumococcal cop Operon.
mSphere: 2017, 2(5);
[PubMed:29062896] [WorldCat.org] [DOI] (P e)

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