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serotype 4
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CGSP14
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Hu15
serotype 19A
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INV104
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INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
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R6
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SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
serotype 3
SPN994039
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SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 24-JAN-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae CGSP14
  • locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
  • pan locus tag?: PNEUPAN003142000
  • symbol: rsmB
  • pan gene symbol?: sun
  • synonym:
  • product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
  • symbol: rsmB
  • product: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
  • replicon: chromosome
  • strand: -
  • coordinates: 1680148..1681461
  • length: 1314
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    GTGACTAAAGTAGAAACGGCTAGAAGTTTAGCTCTAGCAGTGCTAGAGGATGTTTTTGTG
    AACCAAGCATATTCAAATATCGCCTTAAATAAACACCTCAAGGGGAGTCAGCTCTCTGCA
    GCAGACAAGGGCTTAGTGACCGAGCTAGTCTATGGAACGGTAGCCCGTAAACTGACTCTG
    GAATGGTACCTATCCCACTTTATCGAAGACAGAGACCAGTTAGACAGCTGGCTCTATGTC
    CTTCTTCTCATGAGTGTCTACCAACTCCGCTATTTGGACAAGATTCCAGATCATGCTGTG
    GTCAATGAAGCAGTGGAATTGGCCAAACTCCGTAAAAAAGGCAGTGAAAAATTGGTCAAC
    GCTGTCCTTCGCCGTATCTTGCGTGAAGGCTGGCCAGATATTGCTAACATCAAGCGAAAA
    AACAAGCGTGACTCCATTGCCTATTCTCTCCCAGTTTGGCTAGTTACCAAACTCAAGGAA
    GAATACGGAGAGGAGAGAGCAAAAGCCATCTTTGAAAGCTTGTTAGTGCGAAACAAAGCT
    AGTATTCGTGTAACAGACCTAAGTCGAAAAGAGGAAATCCAAGCCTTGTTGGAGGCGAAT
    AGTTCCCTTTTAGCAACCACTGGTCTGGTTAAGGAGCAAGGGCATTTTGCAGGGCATAAT
    TTGTTTGCGGATGGAGCCATTACCATCCAAGATGAGTCCAGTCAGCTGGTTGCTCCGACG
    CTTGATTTACAAGGTGATGAGCGAGTGCTTGATGCCTGTGCGGCTCCAGGTGGGAAAACA
    GCCCATATAGCCTCTTATCTTACGACAGGTCAGGTTACTGCTCTGGACTTGTACGACCAC
    AAGTTGGATTTAATTCAAGAAAATGCCCAACGTCTGGGAGTTGCAGATCGGGTTCAAACT
    CAAAAATTGGATGCCAGAAAGGTGCATGAGTTTTTTGACCAGGATTCCTTTGATAAGATT
    TTGGTGGATGCTCCTTGTTCAGGAATCGGTCTTTTGCGCCGAAAACCAGATATCAAATAC
    AATAAAGAAACGGCAGATTTCGCGTCCTTGCAGGAAATTCAGTTAGAAATATTAGGTAGT
    GTTTGTCAAATACTAGGCAAAGGTGGTATAATAACTTATAGCACCTGCACTATCGTCTCA
    GAGGAGAATTTTCAAGTCGTTAAGGCCTTTTTAGAGAGTCATCCCGAGTTCGAGCAGGTA
    AAACTAGAACATGAATGTAAGGATATCATGAAAGACGGCTGTATCCTCATTACACCTGAA
    TTGTATGGAAGTGATGGATTCTTCATCAGTCAATTTCGCAAGATATCGGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPCG_RS08835 [old locus tag: SPCG_1707 ]
  • symbol: RsmB
  • description: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB
  • length: 437
  • theoretical pI: 5.8625
  • theoretical MW: 48998.8
  • GRAVY: -0.169794

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.176?  ExPASy
    16S rRNA (cytosine967-C5)-methyltransferase S-adenosyl-L-methionine + cytosine967 in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 380)
    and 10 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification NOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 146.8)
    Genetic information processing Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 53.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 25.5)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 23.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 22.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 19.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 17.4)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 16.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll C-20 methyltransferase BchU (TIGR02716; EC 2.1.1.-; HMM-score: 13.1)
    ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 11.9)
  • TheSEED: see SPCG_1707
  • PFAM:
    NADP_Rossmann (CL0063) Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 182.5)
    and 13 more
    NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 100.4)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 31.5)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.8)
    FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 26.5)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 26.1)
    Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 19.6)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 18.3)
    Met_10; Met-10+ like-protein (PF02475; HMM-score: 18)
    Methyltr_RsmF_N; N-terminal domain of 16S rRNA methyltransferase RsmF (PF17125; HMM-score: 15.1)
    TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 14.9)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 13.9)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 12.9)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00902
    • TAT(Tat/SPI): 0.001032
    • LIPO(Sec/SPII): 0.000968
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKGLVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSVYQLRYLDKIPDHAVVNEAVELAKLRKKGSEKLVNAVLRRILREGWPDIANIKRKNKRDSIAYSLPVWLVTKLKEEYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANSSLLATTGLVKEQGHFAGHNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALDLYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRRKPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLESHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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