PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 16-DEC-2024

⊟Summary

• organism: Streptococcus pneumoniae CGSP14
• locus tag: SPCG_RS10140 [old locus tag: SPCG_1933 ]
• pan locus tag^?: PNEUPAN003587000
• symbol: rsmD
• pan gene symbol^?: rsmD
• synonym:
• product: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPCG_RS10140 [old locus tag: SPCG_1933 ]
• symbol: rsmD
• product: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD
• replicon: chromosome
• strand: -
• coordinates: 1919720..1920259
• length: 540
• essential: unknown other strains

⊟Accession numbers

• Location: NC_010582 (1919720..1920259) NCBI
• BioCyc: SPCG_RS10140 BioCyc
• MicrobesOnline: see SPCG_1933
• PneumoBrowse for strain D39V: SPV_1767 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGAAAATCGTATCAGGAATCTATGGGGGACGTTCCCTCAAGACACTAGAAGGCAAGACA
  ACAAGACCTACTTCGGATAAGGTTAGGGGAGCCATTTTTAACATGATTGGTCCCTACTTT
  GAAGGGGGACGAGTCTTGGACCTTTATGCAGGTAGTGGTGGTTTATCTATCGAAGCAGTA
  TCGCGTGGCATGTCCAGTGCTGTTTTGGTGGAGCGAGACCGTAAGGCTCAGACCATCGTT
  GCTGAAAATATCCAGATGACCAAGGAAATTGGAAAATTTCAACTCCTCAAGATGGATGCA
  GAAAGGGCATTGGAACGGGTATCTGGAGAATTTGACCTCGTTTTCTTAGACCCTCCCTAT
  ACCAAGGAACAAATCGTAGCAGATATTGAAAAAATGGCTGAGAGAGAGCTTTTTTCTGAA
  GATGTTATGGTTGTGTGCGAGACGGATAAGGCCGTTGAACTTCCAGAAGAAATTGCCTGT
  CTGGGTATCTGGAAGGAAAAGATTTATGGAATTAGTAAGGTGACAGTCTATGTCAGATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  

⊟Protein

⊟General

• locus tag: SPCG_RS10140 [old locus tag: SPCG_1933 ]
• symbol: RsmD
• description: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD
• length: 179
• theoretical pI: 4.97066
• theoretical MW: 19906
• GRAVY: -0.0949721

⊟Function

• reaction:
  EC 2.1.1.171?  ExPASy
  16S rRNA (guanine⁹⁶⁶-N²)-methyltransferase S-adenosyl-L-methionine + guanine⁹⁶⁶ in 16S rRNA = S-adenosyl-L-homocysteine + N²-methylguanine⁹⁶⁶ in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 172.2)
  and 11 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 31.5)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 27.5)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 26.3)
  Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 22.2)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 19)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 15.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification NOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 15.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 15)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 14.8)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA adenine methylase (TIGR00571; EC 2.1.1.72; HMM-score: 14.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 13.4)
• TheSEED: see SPCG_1933
• PFAM:
  NADP_Rossmann (CL0063) Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 207.1)
  and 16 more
  MTS; Methyltransferase small domain (PF05175; HMM-score: 25.1)
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 21.3)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 20.4)
  MethyltransfD12; D12 class N6 adenine-specific DNA methyltransferase (PF02086; HMM-score: 19.4)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 18.6)
  Methyltrans_SAM; S-adenosylmethionine-dependent methyltransferase (PF10672; HMM-score: 18.3)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 17.7)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 17.4)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 15.3)
  Met_10; Met-10+ like-protein (PF02475; HMM-score: 15.3)
  RsmJ; Ribosomal RNA large subunit methyltransferase D, RlmJ (PF04378; HMM-score: 14.7)
  SAM_MT; Putative SAM-dependent methyltransferase (PF04445; HMM-score: 14.6)
  TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 14.4)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 14.3)
  DNA_methylase; C-5 cytosine-specific DNA methylase (PF00145; HMM-score: 13.3)
  Methyltransf_30; S-adenosyl-L-methionine-dependent methyltransferase (PF05430; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9982
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0015
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025323
  • TAT(Tat/SPI): 0.001757
  • LIPO(Sec/SPII): 0.001781
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000706966 NCBI
• UniProt:

⊟Protein sequence

• MKIVSGIYGGRSLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGRVLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEIGKFQLLKMDAERALERVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIACLGIWKEKIYGISKVTVYVR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPCG_RS10130 < coaD < rsmD < asnA

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1767 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications