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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0458 [new locus tag: SPD_RS02465 ]
  • pan locus tag?: PNEUPAN001402000
  • symbol: hrcA
  • pan gene symbol?: hrcA
  • synonym:
  • product: heat-inducible transcription repressor HrcA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0458 [new locus tag: SPD_RS02465 ]
  • symbol: hrcA
  • product: heat-inducible transcription repressor HrcA
  • replicon: chromosome
  • strand: +
  • coordinates: 467524..468558
  • length: 1035
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTTACAGAGCGTCAGCAGGATATTTTAAATCTGATTATTGACATCTTTACCAAAACG
    CACGAACCTGTCGGATCAAAAGCCTTGCAAGAGTCTATTAACTCTAGCAGTGCAACCATT
    CGTAATGACATGGCGGAACTAGAAAAACAAGGGTTGCTTGAGAAGGCTCATACTTCAAGC
    GGTCGGATGCCAAGTGTTGCTGGTTTTCAGTACTATGTGAAACACTCACTGGATTTTGAC
    CGACTGGCTGAAAATGAGGTATATGAGATTGTCAAAGCCTTTGATCAGGAATTCTTCAAA
    TTGGAGGATATTCTGCAAGAGGCTGCTAACTTGCTAACAGACCTGAGTGGCTGTACGGTA
    GTGGCACTGGATGTTGAGCCGAGCAGGCAACGTTTGACAGCCTTTGATATCGTTGTTTTG
    GGGCAACATACAGCCTTGGCGGTATTTACCCTAGACGAGTCGCGAACGGTTACTAGTCAG
    TTTCTGATTCCAAGGAACTTCTTGCAGGAGGATTTGCTGAAACTGAAGAGCATCATTCAG
    GAACGTTTCCTCGGTCACACCGTTTTAGATATTCACTACAAGATTCGGACGGAGATTCCG
    CAGATTATCCAGCGTTACTTTACAACAACGGATAATGTCATCGATCTCTTTGAACACATC
    TTTAAGGAAATGTTCAACGAAAACATTGTGATGGCGGGCAAGGTCAATCTCTTGAATTTT
    GCCAATCTAGCAGCCTATCAGTTCTTTGACCAACCGCAAAAGGTGGCCTTGGAGATTCGT
    GAGGGGTTGCGTGAGGATCAGATGCAAAATGTTCGTGTTGCAGACGGTCAAGAGTCCTGT
    TTAGCTGACCTAGCGGTGATTAGTAGTAAGTTCCTCATTCCTTATCGGGGAGTTGGAATT
    CTAGCCATTATCGGTCCAGTTAATCTGGATTACCAACAGCTAATCAATCAAATCAATGTG
    GTCAACCGTGTTTTGACCATGAAGTTGACAGATTTTTACCGCTACCTCAGCAGTAATCAT
    TACGAAGTACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1035

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0458 [new locus tag: SPD_RS02465 ]
  • symbol: HrcA
  • description: heat-inducible transcription repressor HrcA
  • length: 344
  • theoretical pI: 4.91378
  • theoretical MW: 39367.8
  • GRAVY: -0.069186

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions heat-inducible transcription repressor HrcA (TIGR00331; HMM-score: 314.3)
    and 3 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 19.3)
    Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 19.3)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.6)
  • TheSEED  :
    • Heat-inducible transcription repressor HrcA
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Heat-inducible transcription repressor HrcA
  • PFAM:
    GAF (CL0161) HrcA; HrcA protein C terminal domain (PF01628; HMM-score: 108.8)
    and 10 more
    HTH (CL0123) HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 29.4)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 27.1)
    HTH_11; HTH domain (PF08279; HMM-score: 24.1)
    LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 18.5)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 16.3)
    HTH_12; Ribonuclease R winged-helix domain (PF08461; HMM-score: 16.1)
    Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 13.3)
    FUR; Ferric uptake regulator family (PF01475; HMM-score: 13)
    MarR; MarR family (PF01047; HMM-score: 12.7)
    Cyclin (CL0065) DUF3452; Domain of unknown function (DUF3452) (PF11934; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Heat shock
  • genes regulated by HrcA, TF important in Heat shock responseRegPrecise
    repression
    hrcA > grpE
    groL < groES
    transcription units transferred from TIGR4 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011174
    • TAT(Tat/SPI): 0.002104
    • LIPO(Sec/SPII): 0.001008
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDMAELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLEDILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQFLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFEHIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADGQESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYRYLSSNHYEVH

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HrcA (repression) regulon
    HrcA(TF)important in Heat shock response; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Su-Nam Kim, Yong-Goo Bae, Dong-Kwon Rhee
Dual regulation of dnaK and groE operons by HrcA and Ca++ in Streptococcus pneumoniae.
Arch Pharm Res: 2008, 31(4);462-7
[PubMed:18449503] [WorldCat.org] [DOI] (P p)

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