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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_1542 [new locus tag: SPD_RS08205 ]
  • pan locus tag?: PNEUPAN003140000
  • symbol: SPD_1542
  • pan gene symbol?: stkP
  • synonym: pknB
  • product: serine/threonine protein kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_1542 [new locus tag: SPD_RS08205 ]
  • symbol: SPD_1542
  • product: serine/threonine protein kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1563636..1565615
  • length: 1980
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGATCCAAATCGGCAAGATTTTTGCCGGACGCTATCGGATTGTCAAACAGATTGGTCGA
    GGAGGCATGGCGGATGTCTACCTAGCCAAAGACTTAATCTTAGATGGGGAAGAAGTGGCA
    GTGAAGGTTCTGAGGACCAACTACCAGACGGACCCGATAGCTGTAGCTCGTTTTCAGCGT
    GAAGCGAGAGCTATGGCAGATCTAGACCATCCTCATATCGTTCGGATAACAGATATTGGT
    GAGGAAGACGGTCAACAGTATCTTGCAATGGAGTATGTTGCTGGACTAGACCTCAAACGC
    TATATCAAGGAACATTATCCTCTTTCTAATGAAGAAGCAGTCCGTATCATGGGACAAATT
    CTCTTGGCTATGCGCTTGGCCCATACTCGAGGAATTGTTCACAGGGACTTGAAACCTCAA
    AATATCCTTTTGACACCAGATGGGACTGCCAAGGTCACAGACTTTGGGATTGCTGTAGCC
    TTTGCAGAGACAAGTCTGACCCAGACTAACTCGATGTTGGGCTCAGTTCATTACTTGTCA
    CCAGAGCAGGCGCGTGGTTCGAAGGCGACTGTGCAGAGTGATATCTATGCCATGGGGATT
    ATTTTCTATGAGATGTTGACAGGCCATATCCCTTATGACGGGGATAGCGCGGTGACCATT
    GCCCTCCAGCATTTCCAGAACCCCCTGCCGTCCGTTATTGCAGAAAATTCATCTGTACCT
    CAGGCTTTAGAAAATGTTATTATCAAGGCAACTGCTAAAAAGTTGACCAATCGCTATCGC
    TCGGTTTCAGAGATGTATGTAGACTTGTCTAGTAGCTTGTCCTACAATCGTAGAAATGAA
    AGTAAGTTAATCTTTGATGAAACGAGCAAGGCAGATACCAAGACCTTGCCGAAGGTTTCT
    CAGAGTACCTTGACATCTATTCCTAAGGTTCAAGCGCAGACAGAACACAAATCAATCAAA
    AACCCAAGCCAGGCTGTGACAGAGGAAACTTACCAACCACAAGCACCGAAAAAACATAGA
    TTTAAGATGCGTTACCTGATTTTGTTGGCCAGCCTTGTATTGGTGGCAGCTTCTCTTATT
    TGGATACTATCCAGAACTCCTGCAACCATTGCCATTCCAGATGTGGCAGGTCAGACAGTT
    GCAGAGGCCAAGGCAACGCTCAAAAAAGCCAATTTTGAGATTGGTGAGGAGAAGACAGAG
    GCTAGTGAAAAGGTGGAAGAAGGGCGGATTATCCGTACAGATCCTGGCGCTGGAACTGGT
    CGAAAAGAAGGAACGAAAATTAATCTGGTTGTCTCATCAGGCAAACAATCCTTCCAAATT
    AGTAATTATGTCGGCCGGAAATCTTCTGATGTTATCGCGGAATTAAAAGAGAAAAAAGTT
    CCAGATAATTTGATTAAAATTGAGGAAGAAGAGTCGAATGAAAGTGAGGCTGGAACGGTC
    CTGAAGCAAAGTCTACCAGAAGGTACGACCTATGACTTGAGCAAGGCAACTCAAATTGTT
    TTGACAGTAGCTAAAAAAGCTACGACGATTCAATTAGGGAACTATATTGGACGGAACTCT
    ACAGAAGTAATCTCAGAACTCAAGCAGAAGAAGGTTCCTGAGAATTTGATTAAGATAGAG
    GAAGAAGAGTCCAGCGAAAGCGAACCAGGAACGATTATGAAACAAAGTCCAGGTGCCGGA
    ACGACTTATGATGTGAGTAAACCTACTCAAATTGTCTTGACAGTAGCTAAAAAAGTTACA
    AGTGTTGCCATGCCGAGTTACATTGGTTCCAGCTTGGAGTTTACTAAGAACAATTTGATT
    CAAATTGTTGGGATTAAGGAAGCTAATATAGAAGTTGTAGAAGTGACGACAGCGCCTGCA
    GGTAGTGTAGAAGGCATGGTTGTTGAACAAAGTCCTAGAGCAGGTGAAAAGGTAGACCTA
    AATAAGACTAGAGTCAAGATTTCAATCTACAAACCTAAAACAACTTCAGCTACTCCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_1542 [new locus tag: SPD_RS08205 ]
  • symbol: SPD_1542
  • description: serine/threonine protein kinase
  • length: 659
  • theoretical pI: 8.97355
  • theoretical MW: 72292.2
  • GRAVY: -0.288771

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 179.8)
    and 2 more
    Unknown function General Kae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 38.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenylphenol 6-hydroxylase (TIGR01982; EC 1.14.13.-; HMM-score: 18.4)
  • TheSEED  :
    • Serine/threonine protein kinase PrkC, regulator of stationary phase
    A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins  Serine/threonine protein kinase PrkC, regulator of stationary phase
    and 1 more
    Conserved gene cluster associated with Met-tRNA formyltransferase  Serine/threonine protein kinase PrkC, regulator of stationary phase
  • PFAM:
    PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 178.5)
    no clan defined PASTA; PASTA domain (PF03793; HMM-score: 143.9)
    and 7 more
    PKinase (CL0016) PK_Tyr_Ser-Thr; Protein tyrosine and serine/threonine kinase (PF07714; HMM-score: 133.2)
    ABC1; ABC1 atypical kinase-like domain (PF03109; HMM-score: 36.3)
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 28.3)
    RIO1; RIO1 family (PF01163; HMM-score: 21.5)
    Kdo; Lipopolysaccharide kinase (Kdo/WaaP) family (PF06293; HMM-score: 21.1)
    Kinase-like; Kinase-like (PF14531; HMM-score: 20.6)
    Pkinase_fungal; Fungal protein kinase (PF17667; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001218
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000273
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVAFAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQNPLPSVIAENSSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVEGMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Linda Nováková, Lenka Sasková, Petra Pallová, Jirí Janecek, Jana Novotná, Ales Ulrych, Jose Echenique, Marie-Claude Trombe, Pavel Branny
Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates.
FEBS J: 2005, 272(5);1243-54
[PubMed:15720398] [WorldCat.org] [DOI] (P p)Lenka Sasková, Linda Nováková, Marek Basler, Pavel Branny
Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae.
J Bacteriol: 2007, 189(11);4168-79
[PubMed:17416671] [WorldCat.org] [DOI] (P p)Andrew T Ulijasz, Shaun P Falk, Bernard Weisblum
Phosphorylation of the RitR DNA-binding domain by a Ser-Thr phosphokinase: implications for global gene regulation in the streptococci.
Mol Microbiol: 2009, 71(2);382-90
[PubMed:19040630] [WorldCat.org] [DOI] (I p)Makoto Osaki, Tania Arcondéguy, Amandine Bastide, Christian Touriol, Hervé Prats, Marie-Claude Trombe
The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization.
J Bacteriol: 2009, 191(15);4943-50
[PubMed:19502404] [WorldCat.org] [DOI] (I p)Linda Nováková, Silvia Bezousková, Petr Pompach, Petra Spidlová, Lenka Sasková, Jaroslav Weiser, Pavel Branny
Identification of multiple substrates of the StkP Ser/Thr protein kinase in Streptococcus pneumoniae.
J Bacteriol: 2010, 192(14);3629-38
[PubMed:20453092] [WorldCat.org] [DOI] (I p)Katrin Beilharz, Linda Nováková, Daniela Fadda, Pavel Branny, Orietta Massidda, Jan-Willem Veening
Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.
Proc Natl Acad Sci U S A: 2012, 109(15);E905-13
[PubMed:22431591] [WorldCat.org] [DOI] (I p)Aurore Fleurie, Sylvie Manuse, Chao Zhao, Nathalie Campo, Caroline Cluzel, Jean-Pierre Lavergne, Céline Freton, Christophe Combet, Sébastien Guiral, Boumediene Soufi, Boris Macek, Erkin Kuru, Michael S VanNieuwenhze, Yves V Brun, Anne-Marie Di Guilmi, Jean-Pierre Claverys, Anne Galinier, Christophe Grangeasse
Interplay of the serine/threonine-kinase StkP and the paralogs DivIVA and GpsB in pneumococcal cell elongation and division.
PLoS Genet: 2014, 10(4);e1004275
[PubMed:24722178] [WorldCat.org] [DOI] (I e)Jenny A Herbert, Andrea M Mitchell, Timothy J Mitchell
A Serine-Threonine Kinase (StkP) Regulates Expression of the Pneumococcal Pilus and Modulates Bacterial Adherence to Human Epithelial and Endothelial Cells In Vitro.
PLoS One: 2015, 10(6);e0127212
[PubMed:26090876] [WorldCat.org] [DOI] (I e)

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