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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 21-MAR-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_RS03230 [old locus tag: SPD_0598 ]
  • pan locus tag?: PNEUPAN001603000
  • symbol: murD
  • pan gene symbol?: murD
  • synonym:
  • product: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_RS03230 [old locus tag: SPD_0598 ]
  • symbol: murD
  • product: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 622903..624255
  • length: 1353
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAGTAATAGATCAATTTAAAAATAAGAAAGTTCTTGTTTTAGGTTTGGCCAAGTCT
    GGTGAATCTGCAGCTCGTTTGTTGGACAAGCTAGGTGCCATTGTGACAGTAAATGATGGG
    AAACCTTTCGAGGACAATCCAGCTGCCCAAAGTTTGCTGGAAGAAGGGATCAAGGTCATT
    ACAGGTGGCCATCCTTTGGAACTCTTGGATGAAGAGTTTGCCCTTATGGTGAAAAATCCA
    GGTATCCCCTACAACAATCCCATGATTGAAAAGGCTTTGGCCAAGAGAATTCCAGTCTTG
    ACTGAGGTGGAATTGGCTTATTTGATTTCAGAAGCACCGATTATTGGTATCACAGGATCG
    AACGGTAAGACAACCACAACGACTATGATTGGGGAAGTTTTGACTGCTGCTGGGCAACAT
    GGTCTTTTATCAGGGAATATCGGCTATCCTGCCAGTCAGGTTGCTCAAATAGCATCAGAT
    AAGGACACGCTTGTTATGGAACTTTCTTCTTTCCAACTCATGGGTGTTCAAGAATTCCAT
    CCAGAGATTGCGGTTATTACCAACCTCATGCCAACTCATATCGACTACCATGGGTCATTT
    TCTGAATATGTAGCAGCCAAGTGGAATATCCAGAACAAGATGACAGCAGCTGATTTCCTT
    GTCTTGAACTTTAATCAAGACTTGGCAAAAGACTTGACTTCCAAGACAGAAGCCACTGTT
    GTACCATTTTCAACACTTGAAAAGGTTGATGGAGCTTATCTGGAAGATGGTCAACTCTAC
    TTCCGTGGTGAAGTAGTCATGGCAGCGAATGAAATCGGTGTTCCAGGTAGCCACAATGTG
    GAAAATGCCCTTGCGACTATTGCTGTAGCCAAGCTTCGTGATGTGGACAATCAAACCATC
    AAGGAAACTCTTTCAGCCTTCGGTGGTGTCAAACACCGTCTCCAGTTTGTGGATGACATC
    AAGGGTGTTAAATTCTATAACGACAGTAAATCAACTAATATCTTGGCTACTCAAAAAGCC
    TTATCAGGATTTGACAACAGCAAGGTCGTCTTGATTGCAGGTGGTTTGGACCGTGGCAAT
    GAGTTTGACGAATTGGTGCCAGACATTACTGGACTCAAGAAGATGGTCATCCTGGGTCAA
    TCTGCAGAACGTGTCAAACGGGCAGCAGACAAGGCTGGTGTCGCTTATGTGGAGGCGACA
    GATATTGCAGATGCGACCCGCAAGGCCTATGAGCTTGCGACTCAAGGAGATGTGGTTCTT
    CTTAGTCCTGCCAATGCCAGCTGGGATATGTATGCTAACTTTGAAGTACGTGGCGACCTC
    TTTATCGACACAGTAGCGGAGTTAAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1353

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_RS03230 [old locus tag: SPD_0598 ]
  • symbol: MurD
  • description: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
  • length: 450
  • theoretical pI: 4.65843
  • theoretical MW: 48579.1
  • GRAVY: -0.0173333

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.9?  ExPASy
    UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 415.5)
    and 8 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 156.3)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 105.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 83.1)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 60.7)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 24)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 21.1)
    Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 17.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, A subunit (TIGR01042; EC 3.6.3.14; HMM-score: 12.7)
  • TheSEED: see SPD_0598
  • PFAM:
    P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 112.5)
    and 5 more
    no clan defined Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 44.1)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 18.9)
    Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 16.1)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 14.1)
    no clan defined TraC; TraC-like protein (PF07820; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9981
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0012
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020946
    • TAT(Tat/SPI): 0.000544
    • LIPO(Sec/SPII): 0.003111
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFEDNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKRIPVLTEVELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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