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serotype 4
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serotype 19A
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serotype 1
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serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
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R6
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serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_0752 [new locus tag: SPG_RS03800 ]
  • pan locus tag?: PNEUPAN001800000
  • symbol: pnp
  • pan gene symbol?: pnp_2
  • synonym:
  • product: purine nucleoside phosphorylase I, inosine and guanosine-specific

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_0752 [new locus tag: SPG_RS03800 ]
  • symbol: pnp
  • product: purine nucleoside phosphorylase I, inosine and guanosine-specific
  • replicon: chromosome
  • strand: +
  • coordinates: 731649..732458
  • length: 810
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACATTTTTAAACAAAATCCATGAAACTGCTACTTTCCTGAAAGAAAAGGGAATTGCA
    GCCCCTGAGTTCGGTCTAATCCTTGGGTCAGGACTTGGAGAATTGGCAGAAGAAATCGAA
    AATCCAGTTGTAGTAGACTATGCTGAGATTCCAAACTGGGGCCGTTCAACAGTAGTCGGT
    CATGCTGGTAAATTGGTATATGGTGAACTGGCAGGTCGCAAGGTCTTGGCTCTTCAAGGG
    CGTTTCCATTTCTATGAAGGGAATCCTCTGGAAGTGGTGACTTTCCCAGTTCGTGTGATG
    AAAGTTCTTGGATGTGAAGGTGTTATTGTAACCAATGCAGCTGGCGGTATCAGATTTGGT
    CCTGGTACCTTGATGGCTATCTCAGACCATATCAACATGACGGGGCAAAATCCATTGATG
    GGGGAAAACTTGGATGACTTTGGCCCACGTTTCCCAGATATGTCTAGGGCCTACACACCA
    GAATACCGTGCCACTGCCCATGAAGTGGCTAAAAAACTTAATATCAAGATTGATGAAGGT
    GTCTATATCGGAGTTACTGGTCCGACTTATGAAACACCAGCAGAAATTCGTTCCTATAAG
    ACACTGGGAGCAGATGCAGTTGGTATGTCTACGGTTCCTGAAGTTATCGTGGCAGCCCAC
    TCTGGCTTGAAAGTTCTGGGAATTTCATGTATCACTAACTTTGCGGCTGGTTTCCAAGAA
    GAACTCAATCACGAAGAAGTTGTAGAAGTGACTGAACGTGTTAAAGGTGATTTCAAAGGC
    TTGCTTAAAGCGATTCTTGCTGAATTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    810

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_0752 [new locus tag: SPG_RS03800 ]
  • symbol: Pnp
  • description: purine nucleoside phosphorylase I, inosine and guanosine-specific
  • length: 269
  • theoretical pI: 5.04237
  • theoretical MW: 29003.1
  • GRAVY: 0.0713755

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.1?  ExPASy
    Purine-nucleoside phosphorylase Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides purine nucleoside phosphorylase I, inosine and guanosine-specific (TIGR01700; EC 2.4.2.1; HMM-score: 346.6)
    inosine/guanosine/xanthosine phosphorylase family (TIGR01697; EC 2.4.2.1; HMM-score: 342.5)
    and 4 more
    purine nucleotide phosphorylase (TIGR01698; EC 2.4.2.1; HMM-score: 263.9)
    xanthosine phosphorylase (TIGR01699; EC 2.4.2.1; HMM-score: 243.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides methylthioadenosine phosphorylase (TIGR01694; EC 2.4.2.28; HMM-score: 77.3)
    Metabolism Central intermediary metabolism Other methylthioadenosine phosphorylase (TIGR01694; EC 2.4.2.28; HMM-score: 77.3)
  • TheSEED  :
    • N-Ribosylnicotinamide phosphorylase (EC 2.4.2.1)
    • Purine nucleoside phosphorylase (EC 2.4.2.1)
    Carbohydrates Monosaccharides Deoxyribose and Deoxynucleoside Catabolism  Purine nucleoside phosphorylase (EC 2.4.2.1)
    and 3 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  N-Ribosylnicotinamide phosphorylase (EC 2.4.2.1)
    Nucleosides and Nucleotides Purines Purine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
  • PFAM:
    PUP (CL0408) PNP_UDP_1; Phosphorylase superfamily (PF01048; HMM-score: 166.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011326
    • TAT(Tat/SPI): 0.001102
    • LIPO(Sec/SPII): 0.001155
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55793 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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