From PneumoWiki
Jump to navigation Jump to search
TIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_2101
  • pan locus tag?:
  • symbol: SPG_2101
  • pan gene symbol?: —
  • synonym:
  • product: blood group cleaving endo-beta-galactosidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_2101
  • symbol: SPG_2101
  • product: blood group cleaving endo-beta-galactosidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1989921..1991651
  • length: 1731
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP001015 (1989921..1991651) NCBI
  • BioCyc:
  • MicrobesOnline: 5757367 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAAGTAAAAAAATAGCAAAACAGATTTTAATAGCTACGGCTGTTTTAACAAGTTTT
    TTAGGAAGTAATTTGGTTTATGCAGATGTAGTTCAAAGTAATTCTAATAACAGAGCTAGT
    ACTGAAACTGCTAGAGTAACAGGGAATAACTTAGAAAAACTTATTACTAAAGATAAAGAA
    ATTGATAAAGAGATGACATATCTTTCAGATATGGATTGGTCTTCTGCAACTCATGGTGAT
    ATAGATAAAACAAAAACAGTACAAAAAGATGCTCCATTTACAACAGGAAATAAAGGAGAG
    CATACTAAGATTTCATTGCTCACTTCTGATGATAAAGTTAAATATTTTGATAAAGGAATA
    GGAACAGTCGCAGATTCTCCATCAGTAATCAGCTATGATATCAGCGGTCAAGGGTTTGAA
    AAATTTGAAACTTATATAGGAATTGATCAAAGTGCTAATAGTTCTAGATCAGACCATGCA
    GTAGTGGATAGAATAGAAATAGAAATTGATGGAAAGGTTGTCTATTTTAGTAGTGTAACT
    AATCCAGAAGGATTCAGATATAATACCCAGGCACAATTTATTAGTGTGACAATTCCACAA
    AACGCTAAAAAAATATCCTTAAAATCATTCGCAGGAGAACATACATGGGGTGATGAGGTT
    GTATTTGCTGATGCTAAACTTATTAAAACTGTTAGTACTCAAACTATAACTCCAGATCTT
    CTTAATAAAGGAATTAATGGAGGAGTATATCTTTCTGATTTAGAATGGGTTGATGCTACT
    CATGGTGATGACGATAAATCAAAAACAGTTCAGAAAGATAAACCGTTTACACCTGGTAAT
    AACGGGTCTAATAATAAAATAAAATTACTAATTGACGGTAAGGAAGTCGAATTTAATAAG
    GGATTGGGAACTGTAGCAAGTAACCCGTCTAGTATAAAATATGATGTTTCGGGTGCTAAT
    GTTACAAGATTTATTTCCTATGTCGGAATTGATAGGAGTGCAAATCATTTAAATTCAGAT
    TACGCAGATATTCAAAAATTTGAAGTTGTTGCGGATGGTAAAGTCATTTACTCATCAGAT
    TCAAAATATCCAAAAGGAATTAAGTATGATACATCGGCATTTCTGGTTGATGTTGAAATT
    CCCAAAGATACACAGACAATAGAACTTAAATCATATTCAGGGAAACATACATGGGCTGAC
    GAATTGGTTTTAGGTGGAGCCTTGTTTATGGCAAATGGTAAATTTAAGAATCCTAATGAT
    TGGTCAGAAGTTGATAAACGTCGTGAAATTAATAATGAACATCCGTTACTTATGATGCCT
    TTATATGCTAACGGAGAAGAGTTTAACCAAGGGAAATATACTTTTTGGGGAGGAGATACA
    TTAACAGGTAAGTGGGAGAATATACCAGATGACTTAAAACCATATACTGTTATACAGTTA
    CATCCAGATGATCTTCCTAAAAGAGATGGAGCTGCTAGAGATTTTTATGAGCATATGTTA
    GAAGAAGCAGCGAAATATGTAAATCCTAAGACTGGAAAAAATGAACCTATTCCAGTTATT
    CTAACGGTTTACACTGCTGGTAATATGCCTTATTATACTTCGGCACATTGGTTAAGTACC
    AGCTGGATTGATAAAATGTATCAAAAATATCCAAATTTGCATGGTATTTTCAGTACTGAG
    AATTATTGGATTTGGGCAAATGATATTAAAATAAGGCGGCAGATTACTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1731

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_2101
  • symbol: SPG_2101
  • description: blood group cleaving endo-beta-galactosidase
  • length: 576
  • theoretical pI: 5.53788
  • theoretical MW: 64404.6
  • GRAVY: -0.538368

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Fucolectin-related protein
  • PFAM:
    GBD (CL0202) NPCBM; NPCBM/NEW2 domain (PF08305; HMM-score: 141.2)
    no clan defined Glyco_hydro_98M; Glycosyl hydrolase family 98 (PF08306; HMM-score: 120.9)
    and 1 more
    GHD (CL0369) SpuA_C; SpuA C-terminal (PF18033; HMM-score: 18.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.99144
    • TAT(Tat/SPI): 0.002614
    • LIPO(Sec/SPII): 0.00195
    • Cleavage Site: CS pos: 28-29. VYA-DV. Pr: 0.9446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55203 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETARVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHTKISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHAVVDRIEIEIDGKVVYFSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGDEVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPFTPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSANHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSYSGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQGKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVNPKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWANDIKIRRQIT

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA* regulon, FucR* (repression) regulon
    CcpA*(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    FucR*(TF)important in Fucose utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPG_2101&oldid=75085"