From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_2102 [new locus tag: SPG_RS10505 ]
  • pan locus tag?: PNEUPAN000194000
  • symbol: SPG_2102
  • pan gene symbol?: galA
  • synonym:
  • product: glycosyl hydrolase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_2102 [new locus tag: SPG_RS10505 ]
  • symbol: SPG_2102
  • product: glycosyl hydrolase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1991662..1993878
  • length: 2217
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACGATTTATATTAATAAGGACGAGACCGTTTTTCATTTGGCAATGAAAGATAGTAGT
    TATATTTTTAGAATTTTAGAAAATGGGGAACTTCAACATCTACATTTTGGGAAAAGGATT
    CATGTCAAGGAAAATTATAACCAATTGATGGCCTATGAAAAAAGAGGATTTGAAGTATCT
    TTTTCTGAAGAATTTGAGGATATTCAACAGTCTATGATACAAAATGAATATTCTTCATAT
    GGGAAAGGAGATTTTCGGCATCCAGCCTTTCAAGTTCAAGGAATGAATGGTAGTAGGATA
    ACGACACTAAAATATCAAGATTTTGAACTTGAAAAAGGGAAAAATCGTCTTAACTCTCTA
    CCTTCAACATTTGATGATATTGGTCAGTGTGCGGAAACATTAACGATTATTTTAACAGAT
    TCCATATTAGATTTAACTGTTAGACTAAATTATACAATTTTTCCGGAATACAATGTCTTA
    GTTAGAAATACGGAATTTTTAAATAATAGCAATAATAAGTTGACTCTTTTGAAAGCAATG
    AGCTTACAGCTAGATCTACCTGATAGTCAATATGACTTTATTCAATTTTCTGGAGCATGG
    CTGAGGGAACGTCAGTTATATAGAACTTCGCTTAGACCAGGTATTCAAGCAATAGATAGC
    TTGAGATACTCATCAAGTCCTCAGCAAAATCCTTTCTTTATGCTATCAAGGAGGGAAACT
    ACAGAGCATAGTGGTGAGGTTTATGGTTTTAACTTTATCTATTCTGGAAATTTTCAAAAT
    ATGATTGAAGTTGACCATTTTGACACCGCTAGAGTAACGGTAGGAATAAATCCAGTAGAA
    TTTCGTTTTTTATTAAATCCTGCCGAAAGTTTTGTGACACCAGAAGCAATTGTGATCTAT
    TCTGATCAAGGGATGAATCAGATGAGCCAACAACTATCAGATTTTTATCGACATCATTTA
    GTTAATCCTAATTTTTCTCAAGCTAGTCGTCCTATAATACTCAATAGTTGGGAAACATTT
    TATTTTGACTTGGGTACAGAAAAAATTTTAGATTTAGCAAAGGCTGCTAAAGATTTAGGG
    ATAGAATTATTTGTACTGGATGATGGTTGGTTTGGTCATAGGAAAGATGACAAAAGTTCT
    CTGGGTGATTGGGTAACAGATAGAAGTCGCCTTCCTGAAGGTATTGGATTTCTTGCAGAT
    GAAATTCACAAAATAGGTTTACAATTTGGTTTGTGGTTTGAGCCTGAAATGATTTCTATT
    GATAGTGATTTGTACAAGAATCACGCCGATTGGACTATCCATTTGTTAGACAGAGAGAAG
    TCAGTAGGAAGAAATCAATATGTGTTGGATTTGACGAGACAGGAAGTTGTTGATTACCTT
    TTTGATTCTATTTCTAAAATCATAATCAAAACAAATCTGGATTATATCAAATGGGATATG
    AATCGTCATATAACAGATATTTATAGTATTGAACTTGATTCTGAACAGCAGATGGAATTT
    GGTCATCGATATATCTTAGGTCTTTATCAGTTATTAGATCGTTTAATAACTAAGTTCCCT
    TCAGTTCTATTTGAATCTTGTTCTTCAGGTGGTGGACGTTTTGATTTAGGACTTATGTAT
    TATGCACCGCAAGCGTGGACGAGTGATGATACGGACCCGATAGAAAGATTGAAAATTCAG
    CATGGAACTTCTTATGGATATTCTCCATCAATGATGACAGCCCATGTTTCTATTTCTCCA
    AATGAACAAAGTGGAAGACAAACGAGTTTGGACACTAGGACAAATGTAGCTTATTTTAGT
    TCTTTCGGTTATGAATTAGATGTTACGAGATTGTCGGTAGAAGAAAAAGAACAAGTGAGA
    GAACAAATTCAGTTTTATAAAAAATATCGTTCATTGTTTCAATATGGGGATTTCTATAGG
    ATAAACAGTCCTTTTAGTTGTGATTCTGCTAGTTGGCAAGTTGTTTCAAAAGATAAATGC
    CAATCGATTTTATTGTATGCTCAATTGAATAGTAAGTTGAATCCAGGTTATACAAGAGTT
    TATTTTAGTGGTTTAGATAAAGATAAATGCTATTCCGTCTCTAGATTTGATGAGTTCTTT
    TATGGAGATGAATTAATGAATGCTGGAATAAAAGTCAGTTTAAGTAATTTAGCACTTTGT
    GTTCCAGAATATCTTACAAAATTATTTGTTATTGAAGAAGTTGTATGTAAATATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2217

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_2102 [new locus tag: SPG_RS10505 ]
  • symbol: SPG_2102
  • description: glycosyl hydrolase family protein
  • length: 738
  • theoretical pI: 4.93971
  • theoretical MW: 85783.1
  • GRAVY: -0.383333

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Alpha-galactosidase (EC 3.2.1.22)
    Carbohydrates Di- and oligosaccharides Fructooligosaccharides(FOS) and Raffinose Utilization  Alpha-galactosidase (EC 3.2.1.22)
    and 1 more
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Alpha-galactosidase (EC 3.2.1.22)
  • PFAM:
    Glyco_hydro_tim (CL0058) Melibiase; Melibiase (PF02065; HMM-score: 516.3)
    and 4 more
    Gal_mutarotase (CL0103) Glyco_hydro_36N; Glycosyl hydrolase family 36 N-terminal domain (PF16875; HMM-score: 262.9)
    GHD (CL0369) Glyco_hydro_36C; Glycosyl hydrolase family 36 C-terminal domain (PF16874; HMM-score: 66)
    Glyco_hydro_tim (CL0058) Melibiase_2; Alpha galactosidase A (PF16499; HMM-score: 26.2)
    Glyco_hydro_31; Glycosyl hydrolases family 31 (PF01055; HMM-score: 21.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000989
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.000217
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF54875 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKENYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITTLKYQDFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVLVRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAIDSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGINPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIILNSWETFYFDLGTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLPEGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQFYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYFSGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA* regulon, FucR* (repression) regulon
    CcpA*(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    FucR*(TF)important in Fucose utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPG_2102&oldid=75086"