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serotype 4
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HU-OH
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Hu15
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Hu17
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INV104
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INV200
serotype 14
JJA
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MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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OXC141
serotype 3
P1031
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R6
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SP49
serotype 19A
SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
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ST556
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TCH8431/19A
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Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_2188 [new locus tag: SPG_RS10925 ]
  • pan locus tag?: PNEUPAN003949000
  • symbol: SPG_2188
  • pan gene symbol?: htrA
  • synonym:
  • product: serine protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_2188 [new locus tag: SPG_RS10925 ]
  • symbol: SPG_2188
  • product: serine protease
  • replicon: chromosome
  • strand: +
  • coordinates: 2076940..2078121
  • length: 1182
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAACATCTAAAAACATTTTACAAAAAATGGTTTCAATTATTAGTCGTTATCGTCATT
    AGCTTTTTTAGTGGAGCCTTGGGTAGTTTTTCAATAACTCAACTAACTCAAAAAAGTAGT
    GTAAACAACTCTAACAACAATAGTACTATTACACAAACTGCCTATAAGAACGAAAATTCA
    ACAACACAGGCTGTTAACAAAGTAAAAGATGCTGTTGTTTCTGTTATTACTTATTCGGCA
    AACAGACAAAATAGCGTATTTGGCAATGATGATACTGACACAGATTCTCAGCGAATCTCT
    AGTGAAGGATCTGGAGTTATTTATAAAAAGAATGATAAAGAAGCTTACATCGTCACCAAC
    AATCACGTTATAAATGGCGCCAGCAAAGTAGATATTAGATTGTCAGATGGGACTAAAGTA
    CCTGGAGAAATTGTCGGAGCTGACACTTTCTCTGATATTGCTGTCGTCAAAATCTCTTCA
    GAAAAAGTGACAACAGTAGCTGAGTTTGGTGATTCTAGTAAGTTAACTGTAGGAGAAACT
    GCTATTGCCATCGGTAGCCCGTTAGGTTCTGAATATGCAAATACTGTCACTCAAGGTATC
    GTATCCAGTCTCAATAGAAATGTATCCTTAAGATCGGAAGATGGACAAGCTATTTCTACA
    AAAGCCATCCAAACTGATACTGCTATTAACCCAGGTAACTCTGGCGGCCCACTGATCAAT
    ATTCAAGGGCAGGTTATCGGAATTACCTCAAGTAAAATTGCTACAAATGGAGGAACATCT
    GTAGAAGGTCTTGGTTTCGCAATTCCTGCAAATGATGCTATCAATATTATTGAACAGTTA
    GAAAAAAACGGAAAAGTGACGCGTCCAGCTTTGGGAATCCAGATGGTTAATTTATCTAAT
    GTGAGTACAAGCGACATCAGAAGACTCAATATTCCAAGTAATGTTACATCTGGTGTAGTT
    GTTCGTTCGGTACAAAGTAATATGCCTGCCAATGGTCACCTTGAAAAATACGATGTAATT
    ACAAAAGTAGATGACAAAGAGATTGCTTCATCAACAGACTTACAAAGTGCTCTTTACAAC
    CATTCTATCGGAGACACCATTAAGATAACCTACTATCGTAACGGGAAAGAAGAAACTACC
    TCTANCAAACTTAACAAGAGTTCAGGTGATTTAGAATCTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_2188 [new locus tag: SPG_RS10925 ]
  • symbol: SPG_2188
  • description: serine protease
  • length: 393
  • theoretical pI: 6.98004
  • theoretical MW: 41742.2
  • GRAVY: -0.272449

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 273.3)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 273.3)
    and 5 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 178.3)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 178.3)
    Signal transduction Regulatory functions Other RNA chaperone Hfq (TIGR02383; HMM-score: 14.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 13.2)
    GLPGLI family protein (TIGR01200; HMM-score: 7)
  • TheSEED  :
    • Serine protease, DegP/HtrA, do-like (EC 3.4.21.-)
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 105.9)
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 87.5)
    and 6 more
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 59.3)
    Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 26.3)
    PDZ-like (CL0466) PDZ; PDZ domain (PF00595; HMM-score: 21.2)
    Peptidase_PA (CL0124) Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 15)
    PDZ-like (CL0466) PDZ_6; PDZ domain (PF17820; HMM-score: 14.1)
    no clan defined FPP; Filament-like plant protein, long coiled-coil (PF05911; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0006
    • Cytoplasmic Membrane Score: 0.8218
    • Cell wall & surface Score: 0.0121
    • Extracellular Score: 0.1654
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.773697
    • TAT(Tat/SPI): 0.010815
    • LIPO(Sec/SPII): 0.170382
    • Cleavage Site: CS pos: 31-32. SFS-IT. Pr: 0.1057
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55645 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSEKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSXKLNKSSGDLES

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Yasser Musa Ibrahim, Alison R Kerr, Jackie McCluskey, Tim J Mitchell
Role of HtrA in the virulence and competence of Streptococcus pneumoniae.
Infect Immun: 2004, 72(6);3584-91
[PubMed:15155668] [WorldCat.org] [DOI] (P p)Marco Cassone, Alyssa L Gagne, Lynn A Spruce, Steven H Seeholzer, Michael E Sebert
The HtrA protease from Streptococcus pneumoniae digests both denatured proteins and the competence-stimulating peptide.
J Biol Chem: 2012, 287(46);38449-59
[PubMed:23012372] [WorldCat.org] [DOI] (I p)Sacha F de Stoppelaar, Hester J Bootsma, Aldert Zomer, Joris J T H Roelofs, Peter W M Hermans, Cornelis van 't Veer, Tom van der Poll
Streptococcus pneumoniae serine protease HtrA, but not SFP or PrtA, is a major virulence factor in pneumonia.
PLoS One: 2013, 8(11);e80062
[PubMed:24244609] [WorldCat.org] [DOI] (I e)

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