PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 10-NOV-2021

⊟Summary

• organism: Streptococcus pneumoniae G54
• locus tag: SPG_RS07720 [old locus tag: SPG_1549 ]
• pan locus tag^?: PNEUPAN003028000
• symbol: SPG_RS07720
• pan gene symbol^?: mntR
• synonym:
• product: metal-dependent transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPG_RS07720 [old locus tag: SPG_1549 ]
• symbol: SPG_RS07720
• product: metal-dependent transcriptional regulator
• replicon: chromosome
• strand: +
• coordinates: 1489037..1489687
• length: 651
• essential: unknown

⊟Accession numbers

• Location: NC_011072 (1489037..1489687) NCBI
• BioCyc: SPG_RS07720 BioCyc
• MicrobesOnline: see SPG_1549
• PneumoBrowse for strain D39V: SPV_1450 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGACCCCAAACAAAGAAGACTATCTAAAATGTATTTATGAAATTGGCATAGACCTGCAT
  AAGATTACCAACAAGGAAATTGCGGCTCGCATGCAAGTCTCTCCCCCTGCCGTAACTGAA
  ATGATCAAACGAATGAAAAGTGAAAATCTCATCCTAAAGGACAAGGAATGTGGCTATCTA
  CTGACTGACCTCGGTCTCAAACTGGTCTCTGAGCTCTATCGTAAGCACCGCTTGATTGAA
  GTTTTTCTAGTTCATCATTTAGACTATACAAGTGACCAGATTCACGAGGAAGCTGAGGTC
  TTGGAACACACTGTCTCTGACCTGTTCGTGGAAAGACTAGATAAACTGCTAGGTTTCCCT
  AAAACCTGCCCCCACGGAGGAACTATTCCTGCCAAGGGAGAACTCCTCGTTGAAATCAAT
  AACCTCCCACTAGCTGATATCAAGGAAGCTGGCGCCTACCGCCTGACTCGGGTGCACGAT
  AGTTTTGACATTCTCCATTATCTGGACAAGCACTCACTTCACATCGGTGACCAGCTCCAA
  GTCAAGCAGTTTGATGGCTTCAGCAATACCTTCACTATCCTCAGTAACGACGAGGATTTA
  CAAGTGAATATAGACATTGCAAAACAACTCTATGTCGAGAAAATCAACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  651
  

⊟Protein

⊟General

• locus tag: SPG_RS07720 [old locus tag: SPG_1549 ]
• symbol: SPG_RS07720
• description: metal-dependent transcriptional regulator
• length: 216
• theoretical pI: 5.74358
• theoretical MW: 24807.4
• GRAVY: -0.256481

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 16.4)
  phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 14.6)
  and 4 more
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 13.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 12.7)
  Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 12.7)
  homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 12.6)
• TheSEED: see SPG_1549
• PFAM:
  HTH (CL0123) Fe_dep_repr_C; Iron dependent repressor, metal binding and dimerisation domain (PF02742; HMM-score: 93.1)
  and 19 more
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 50.2)
  MarR; MarR family (PF01047; HMM-score: 31.2)
  TRB (CL0206) FeoA; FeoA domain (PF04023; HMM-score: 27.9)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 25.1)
  MarR_2; MarR family (PF12802; HMM-score: 23.6)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 22.1)
  HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 18.3)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 13.5)
  HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 13.5)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 13.3)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.2)
  Phage_rep_O; Bacteriophage replication protein O (PF04492; HMM-score: 13.1)
  Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 12.9)
  Sigma70_r3; Sigma-70 region 3 (PF04539; HMM-score: 12.8)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.8)
  no clan defined Herpes_UL14; Herpesvirus UL14-like protein (PF03580; HMM-score: 12.5)
  HTH (CL0123) Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 11.9)
  GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 11.5)
  no clan defined DUF465; Protein of unknown function (DUF465) (PF04325; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by MntR*, TF important in Manganese homeostasis: see SPG_1549

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004829
  • TAT(Tat/SPI): 0.000221
  • LIPO(Sec/SPII): 0.000613
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000188504 NCBI
• UniProt:

⊟Protein sequence

• MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKRMKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLEHTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHDSFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNIDIAKQLYVEKIN

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPG_RS07720 > SPG_RS07730

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1450 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Jason W Johnston, David E Briles, Lisa E Myers, Susan K Hollingshead
Mn2+-dependent regulation of multiple genes in Streptococcus pneumoniae through PsaR and the resultant impact on virulence.
Infect Immun: 2006, 74(2);1171-80
[PubMed:16428766] [WorldCat.org] [DOI] (P p)Tomas G Kloosterman, Robert M Witwicki, Magdalena M van der Kooi-Pol, Jetta J E Bijlsma, Oscar P Kuipers
Opposite effects of Mn2+ and Zn2+ on PsaR-mediated expression of the virulence genes pcpA, prtA, and psaBCA of Streptococcus pneumoniae.
J Bacteriol: 2008, 190(15);5382-93
[PubMed:18515418] [WorldCat.org] [DOI] (I p)Wouter T Hendriksen, Hester J Bootsma, Angela van Diepen, Silvia Estevão, Oscar P Kuipers, Ronald de Groot, Peter W M Hermans
Strain-specific impact of PsaR of Streptococcus pneumoniae on global gene expression and virulence.
Microbiology (Reading): 2009, 155(Pt 5);1569-1579
[PubMed:19372167] [WorldCat.org] [DOI] (P p)Radha Gupta, Minny Bhatty, Edwin Swiatlo, Bindu Nanduri
Role of an iron-dependent transcriptional regulator in the pathogenesis and host response to infection with Streptococcus pneumoniae.
PLoS One: 2013, 8(2);e55157
[PubMed:23437050] [WorldCat.org] [DOI] (I p)Irfan Manzoor, Sulman Shafeeq, Oscar P Kuipers
Ni2+-Dependent and PsaR-Mediated Regulation of the Virulence Genes pcpA, psaBCA, and prtA in Streptococcus pneumoniae.
PLoS One: 2015, 10(11);e0142839
[PubMed:26562538] [WorldCat.org] [DOI] (I e)