⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae G54
- locus tag: SPG_RS09180 [old locus tag: SPG_1836 ]
- pan locus tag?: PNEUPAN003527000
- symbol: ply
- pan gene symbol?: ply
- synonym:
- product: cholesterol-dependent cytolysin pneumolysin
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPG_RS09180 [old locus tag: SPG_1836 ]
- symbol: ply
- product: cholesterol-dependent cytolysin pneumolysin
- replicon: chromosome
- strand: -
- coordinates: 1749821..1751236
- length: 1416
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_011072 (1749821..1751236) NCBI
- BioCyc: SPG_RS09180 BioCyc
- MicrobesOnline: see SPG_1836
- PneumoBrowse for strain D39V: SPV_1726 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGGCAAATAAAGCAGTAAATGACTTTATACTAGCTATGAATTACGATAAAAAGAAACTC
TTGACCCATCAGGGAGAAAGTATTGAAAATCGTTTCATCAAAGAGGGTAATCAGCTACCC
GATGAGTTTGTTGTTATCGAAAGAAAGAAGCGGAGCTTGTCGACAAATACAAGTGATATT
TCTGTAACAGCTACCAACGACAGTCGCCTCTATCCTGGAGCACTTCTCGTAGTGGATGAG
ACCTTGTTAGAGAATAATCCCACTCTTCTTGCGGTCGATCGTGCTCCGATGACTTATAGT
ATTGATTTGCCTGGTTTGGCAAGTAGCGATAGCTTTCTCCAAGTGGAAGACCCCAGCAAT
TCGAGTGTTCGCGGAGCGGTAAACGATTTGTTGGCTAAGTGGCATCAAGATTATGGTCAG
GTCAATAATGTCCCAGCTAGAATGCAGTATGAAAAAATCACGGCTCACAGCATGGAACAA
CTCAAGGTCAAGTTTGGTTCTGACTTTGAAAAGACAGGGAATTCTCTTGATATTGATTTT
AACTCTGTCCATTCAGGCGAAAAGCAGATTCAGATTGTTAATTTTAAGCAGATTTATTAT
ACAGTCAGCGTAGATGCTGTTAAAAATCCAGGAGATGTGTTTCAAGATACTGTAACGGTA
GAGGATTTAAAACAGAGAGGAATTTCTGCAGAGCGTCCTTTGGTCTATATTTCGAGTGTT
GCTTATGGGCGCCAAGTCTATCTCAAGTTGGAAACCACGAGTAAGAGTGATGAAGTAGAG
GCTGCTTTTGAAGCTTTGATAAAAGGAGTCAAGGTAGCTCCTCAGACAGAGTGGAAGCAG
ATTTTGGACAATACAGAAGTGAAGGCGGTTATTTTAGGGGGCGACCCAAGTTCGGGTGCC
CGAGTTGTAACAGGCAAGGTGGATATGGTAGAGGACTTGATTCAAGAAGGCAGTCGCTTT
ACAGCAGATCATCCAGGCTTGCCGATTTCCTATACAACTTCTTTTTTACGTGACAATGTA
GTTGCGACCTTTCAAAATAGTACAGACTATGTTGAGACTAAGGTTACAGCTTACAGAAAC
GGAGATTTACTGCTGGATCATAGTGGTGCCTATGTTGCCCAATATTATATTACTTGGAAT
GAATTATCCTATGATCATCAAGGTAAGGAAGTCTTGACTCCTAAGGCTTGGGACAGAAAT
GGGCAGGATTTAACGGCTCACTTTACCACTAGTATTCCTTTAAAAGGGAATGTTCGTAAT
CTCTCTGTCAAAATTAGAGAGTGTACCGGGCTTGCCTGGGAATGGTGGCGTACGGTTTAT
GAAAAAACCGATTTGCCACTAGTGCGTAAGCGGACGATTTCTATTTGGGGAACAACTCTC
TATCCTCAGGTAGAAGATAAGGTAGAAAATGACTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPG_RS09180 [old locus tag: SPG_1836 ]
- symbol: Ply
- description: cholesterol-dependent cytolysin pneumolysin
- length: 471
- theoretical pI: 4.97899
- theoretical MW: 52898
- GRAVY: -0.419533
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SPG_1836
- PFAM: CDC (CL0293) Thiol_cytolysin; Thiol-activated cytolysin (PF01289; HMM-score: 540.7)and 2 moreno clan defined Thiol_cytolys_C; Thiol-activated cytolysin beta sandwich domain (PF17440; HMM-score: 168.1)Effector_1; Effector from type III secretion system (PF04518; HMM-score: 15.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0.1
- Cytoplasmic Membrane Score: 0.14
- Cellwall Score: 0.15
- Extracellular Score: 9.6
- Internal Helices: 0
- DeepLocPro: Extracellular
- Cytoplasmic Score: 0.0029
- Cytoplasmic Membrane Score: 0.0023
- Cell wall & surface Score: 0.001
- Extracellular Score: 0.9938
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00309
- TAT(Tat/SPI): 0.000696
- LIPO(Sec/SPII): 0.000448
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_001284361 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription[edit | edit source]
- transcription start site:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1726 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
P Balachandran, S K Hollingshead, J C Paton, D E Briles
The autolytic enzyme LytA of Streptococcus pneumoniae is not responsible for releasing pneumolysin.
J Bacteriol: 2001, 183(10);3108-16
[PubMed:11325939] [WorldCat.org] [DOI] (P p)Katherine E Price, Andrew Camilli
Pneumolysin localizes to the cell wall of Streptococcus pneumoniae.
J Bacteriol: 2009, 191(7);2163-8
[PubMed:19168620] [WorldCat.org] [DOI] (I p)C Feldman, T J Mitchell, P W Andrew, G J Boulnois, R C Read, H C Todd, P J Cole, R Wilson
The effect of Streptococcus pneumoniae pneumolysin on human respiratory epithelium in vitro.
Microb Pathog: 1990, 9(4);275-84
[PubMed:2097494] [WorldCat.org] [DOI] (P p)Katherine E Price, Neil G Greene, Andrew Camilli
Export requirements of pneumolysin in Streptococcus pneumoniae.
J Bacteriol: 2012, 194(14);3651-60
[PubMed:22563048] [WorldCat.org] [DOI] (I p)A M Berry, J Yother, D E Briles, D Hansman, J C Paton
Reduced virulence of a defined pneumolysin-negative mutant of Streptococcus pneumoniae.
Infect Immun: 1989, 57(7);2037-42
[PubMed:2731982] [WorldCat.org] [DOI] (P p)Katharina van Pee, Alexander Neuhaus, Edoardo D'Imprima, Deryck J Mills, Werner Kühlbrandt, Özkan Yildiz
CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin.
Elife: 2017, 6;
[PubMed:28323617] [WorldCat.org] [DOI] (I e)Kristin Jahn, Stefan Handtke, Raghavendra Palankar, Sabrina Weißmüller, Geraldine Nouailles, Thomas P Kohler, Jan Wesche, Manfred Rohde, Corina Heinz, Axel F Aschenbrenner, Martina Wolff, Jörg Schüttrumpf, Martin Witzenrath, Sven Hammerschmidt, Andreas Greinacher
Pneumolysin induces platelet destruction, not platelet activation, which can be prevented by immunoglobulin preparations in vitro.
Blood Adv: 2020, 4(24);6315-6326
[PubMed:33351126] [WorldCat.org] [DOI] (I p)K Kanclerski, R Möllby
Production and purification of Streptococcus pneumoniae hemolysin (pneumolysin).
J Clin Microbiol: 1987, 25(2);222-5
[PubMed:3818918] [WorldCat.org] [DOI] (P p)J C Paton, R A Lock, D J Hansman
Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae.
Infect Immun: 1983, 40(2);548-52
[PubMed:6840851] [WorldCat.org] [DOI] (P p)J C Paton
The contribution of pneumolysin to the pathogenicity of Streptococcus pneumoniae.
Trends Microbiol: 1996, 4(3);103-6
[PubMed:8868088] [WorldCat.org] [DOI] (P p)J Rossjohn, R J Gilbert, D Crane, P J Morgan, T J Mitchell, A J Rowe, P W Andrew, J C Paton, R K Tweten, M W Parker
The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumoniae.
J Mol Biol: 1998, 284(2);449-61
[PubMed:9813129] [WorldCat.org] [DOI] (P p)