⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae G54
- locus tag: SPG_RS10905 [old locus tag: SPG_2184 ]
- pan locus tag?: PNEUPAN003934000
- symbol: comD
- pan gene symbol?: comD
- synonym:
- product: competence system sensor histidine kinase ComD
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPG_RS10905 [old locus tag: SPG_2184 ]
- symbol: comD
- product: competence system sensor histidine kinase ComD
- replicon: chromosome
- strand: -
- coordinates: 2074525..2075850
- length: 1326
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_011072 (2074525..2075850) NCBI
- BioCyc: SPG_RS10905 BioCyc
- MicrobesOnline: see SPG_2184
- PneumoBrowse for strain D39V: SPV_2064 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGGATTTATTTGGATTTGGGACGGTTATTGTTCATTTTTTAATTATTAGTCACAGTTAC
CATTTTATTTGTAAAGGTCAAATAAATAGAAAAGAATTATTCGTTTTTGGTGCTTATACA
TTACTAACAGAAATAGTATTTGATTTTCCCTTATATATTCTATATTTAGATGGGTTAGGG
ATTGAAAGATTTTTATTTCCTTTGGGATTATATTCCTATTTTCGATGGATGAAACAGTAT
GAGAGGGATAGAGGACTATTCCTAAGTTTACTACTATCTCTTTTATATGAGAGCACTCAT
AACTTTCTGTCCGTAACTTTCTCCTCTATAACAGGAGATAATTTTGTTTTACAATATCAT
TTCCCATTCTTTTTCGTTGTGACGGTGTTAACCTATTTTGTTACATTAAAAATCATTTAC
TATTTCCATTTGGAACTAGCCTATTTTGACGAGGACTACCTTTATCCTTTCTTGAAAAAA
GTATTTTTTGCTTTACTATTGCTACATATTGTATCTTTCGTTTCAGATATGGTAAGTACG
ATTAAACATTTGAATAGTTTTGGAAGTATTTTGTCATCTATTGTCTTTATCTCTCTCCTT
TTGACCTTCTTTGCAATGAATTCTCATAAAGTTCAAATGGAGAAAGAGATTGCTTTGAAG
CAGAAGAAATTTGAACAGAAACATTTACAGAATTACACAGATGAAATTGTTGGTCTGTAT
AATGAAATCCGTGGTTTTCGACATGATTATGCTGGAATGCTTGTCAGCATGCAGATGGCA
ATTGACAGTGGTAATTTACAGGAAATTGACAGAATTTACAATGAAGTTTTAGTCAAANCN
AATCATAAATTGCGTTCAGATAAGTACACTTACTTTGATTTGAACAACATAGAAGACTCA
GCTTTACGAAGTTTGGTTGCTCAGTCAATTGTCTATGCTCGAAATAATGGTGTAGAGTTT
ACACTGGAAGTAAAAGATACGATTACCAAGCTTCCAATTGAACTATTGGATTTGGTTCGT
ATCATGAGCGTTTTATTGAATAATGCTGTCGAAGGATCGGCTGATAGCTATAAAAAGCAG
ATGGAAGTAGCAGTTATTAAGATGGAAACTGAAACAGTTGTTGTGATTCAGAATTCATGT
AAAATGACGATGACTCCTTCAGGAGATCTATTTGCCTTAGGATTCTCCACTAAGGGAAGA
AATCGCGGAGTCGGATTAAATAATGTGAAAGAACTACTAGATAAGTACAACAATATTATT
TTAGAAACAGAGATGGAAGGCAGTACATTTAGACAAATCATTAGATTTAAGAGGGAATTT
GAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPG_RS10905 [old locus tag: SPG_2184 ]
- symbol: ComD
- description: competence system sensor histidine kinase ComD
- length: 441
- theoretical pI: 6.65561
- theoretical MW: 51268.3
- GRAVY: 0.189318
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SPG_2184
- PFAM: His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 49.9)and 4 moreHATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 27.9)no clan defined DUF6172; Family of unknown function (DUF6172) (PF19669; HMM-score: 12.5)DUF1514; Protein of unknown function (DUF1514) (PF07438; HMM-score: 10.7)DUF6524; Family of unknown function (DUF6524) (PF20134; HMM-score: 10)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009758
- TAT(Tat/SPI): 0.000247
- LIPO(Sec/SPII): 0.014038
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_012512666 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTEIVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKXNHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVVVIQNSCKMTMTPSGDLFALGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SPG_RS10890 < SPG_RS10895 < comE < comD < comC
⊟Regulation[edit | edit source]
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_2064 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
O Ween, P Gaustad, L S Håvarstein
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.
Mol Microbiol: 1999, 33(4);817-27
[PubMed:10447890] [WorldCat.org] [DOI] (P p)Bernard Martin, Chantal Granadel, Nathalie Campo, Vincent Hénard, Marc Prudhomme, Jean-Pierre Claverys
Expression and maintenance of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae.
Mol Microbiol: 2010, 75(6);1513-28
[PubMed:20180906] [WorldCat.org] [DOI] (I p)Claudia Trappetti, Luciana Gualdi, Lorenzo Di Meola, Prashant Jain, Cindy C Korir, Paul Edmonds, Francesco Iannelli, Susanna Ricci, Gianni Pozzi, Marco R Oggioni
The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model.
BMC Microbiol: 2011, 11;75
[PubMed:21492426] [WorldCat.org] [DOI] (I e)M S Chandler, D A Morrison
Competence for genetic transformation in Streptococcus pneumoniae: molecular cloning of com, a competence control locus.
J Bacteriol: 1987, 169(5);2005-11
[PubMed:3032904] [WorldCat.org] [DOI] (P p)L S Håvarstein, G Coomaraswamy, D A Morrison
An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
Proc Natl Acad Sci U S A: 1995, 92(24);11140-4
[PubMed:7479953] [WorldCat.org] [DOI] (P p)E V Pestova, L S Håvarstein, D A Morrison
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system.
Mol Microbiol: 1996, 21(4);853-62
[PubMed:8878046] [WorldCat.org] [DOI] (P p)L S Håvarstein, P Gaustad, I F Nes, D A Morrison
Identification of the streptococcal competence-pheromone receptor.
Mol Microbiol: 1996, 21(4);863-9
[PubMed:8878047] [WorldCat.org] [DOI] (P p)