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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_1270 [new locus tag: SPH_RS06145 ]
  • pan locus tag?: PNEUPAN000159000
  • symbol: SPH_1270
  • pan gene symbol?: bbmA_1
  • synonym:
  • product: neopullulanase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_1270 [new locus tag: SPH_RS06145 ]
  • symbol: SPH_1270
  • product: neopullulanase
  • replicon: chromosome
  • strand: +
  • coordinates: 1177785..1179527
  • length: 1743
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    GTGGAATTAACAGCCATTTACCACAGACCAGAGTCGGAGTATGCCTATCTTTATAAGGAT
    AAGAAACTTCATATTCGGATTCGAACTAAGAAAGGGGATATTGAAAACATCAACTTGCAT
    TATGGTGACCCTTTTATCTTTATGGAGGAGTTTTATCAAGATACAAAGAAAATGGCAAAG
    ATAACTTCTGATGCCTTATTTGACCATTGGCAGGTTGAAGTGTCCGTCGACTTTGCACGT
    ATCCAGTATCTATTTGAACTCAAGGATACAGAAGGTCAAAGTATTTTGTATGGCGATAAA
    GGGTGTGTTGAAAATTCTTTAGAAAATCTTCGTGCTATTGGAAATGGATTTAAGTTGCCC
    TATCTTCATGAGATTGATGCCTGCAAGGTTCCTGACTGGGTTTTAAATACGGTATGGTAT
    CAGATATTTCCTGAGAGATTTGCCAATGGAAATGCTCTATTAAACCCAGAAGGGACATTA
    GACTGGGATTTATCTATCACACCTCAGAGTGATGATTTCTTTGGTGGTGATTTACAGGGG
    ATTATTGACCATCTGGATTATTTGCAAGACTTGGGCATTACTGGACTGTATCTTTGTCCT
    ATCTTTGAATCTACGAGCAATCATAAGTACAATACGACAGATTACTTTGAAATTGACCGT
    CATTTTGGGGACAAGGATACTTTTCGGGAACTGGTCGAGCAAGCGCATCAGCGAGGTATG
    AAAATTATGTTGGATGCAGTATTTAACCATATTGGTTCGCAATCGACTCAATGGCAAGAT
    GTTGTCCAAAATGGTGAACAGTCTGCTTATAAAGATTGGTTCCATATCCAACAATTCCCA
    GTGACTACTGAAAAGCCAGCTAATAAGAGAGACTTACCCTATCATGCTTTTGGTTTCGAG
    GACTATATGCCTAAGCTAAATACGGCCAATCCAGAAGTCAAGGACTATCTTTTAAAGGTT
    GCGACTTATTGGATTGAAGAGTTTGATATCGATGCTTGGCGTTTGGATGTGGCTAATGAG
    ATTGACCATCAGTTTTGGAAGGATTTTCGAAAAGCAGTTTTAGCTAAAAAGCCAGATCTT
    TATATCCTAGGAGAAGTCTGGCATACGTCTCAGCCTTGGTTAAATGGAGATGAGTTCCAT
    GCGGTCATGAATTATCCTTTATCTGATAGTATCAAGGACTATTTCTTACGAGGAATTAAG
    AAGACAGACCAATTCATCGATGAAATCAATTGCCAATCTATGTATTACAAGCAACAGATT
    TCAGAAGTTATGTTTAATCTCTTGGATTCTCATGATACAGAGCGAATCCTGTGGACGGCT
    AATGAGGATGCTCAACTGGTCAAATCAGCTCTAGCCTTTCTCTTTTTACAAAAGGGAACA
    CCGTGTATTTATTACGGAACAGAGTTAGCTTTAACCGGAGGACCGGACCCAGATTGTCGA
    CGTTGTATGCCTTGGGAACGTGTATCAAGTGACAATGAGATGCTGAACTTTATGAAGAGG
    CTGATTAAGATTCGCAAACATGCGTCAGCAACCATTCAGCATGGCAAGCATAGTCTCCAA
    GAAATCAAAGCTGATGTAGTAGCTTTGCAGTGGAAATATGAAGGGCGAATCCTCAAAGCG
    ATTTTCAACCAATCAAAGGAAGATTATTATTTACAAAAAGAAGCAGTAGCACTAGCAAGC
    AATTGCCAAGAAATAGAGAATCAGCTTGTCATCTCTCCAAAAGGATTTGTGATTTTTCAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1743

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_1270 [new locus tag: SPH_RS06145 ]
  • symbol: SPH_1270
  • description: neopullulanase
  • length: 580
  • theoretical pI: 5.02397
  • theoretical MW: 67822.3
  • GRAVY: -0.467931

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.135?  ExPASy
    Neopullulanase Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 112.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 97)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 95.9)
    and 7 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 85.9)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 62.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 52.1)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 45.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 43.7)
    pullulanase, extracellular (TIGR02102; HMM-score: 36.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 20.9)
  • TheSEED  :
    • Neopullulanase (EC 3.2.1.135)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Neopullulanase (EC 3.2.1.135)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 254.5)
    and 3 more
    E-set (CL0159) Alpha-amylase_N; Alpha amylase, N-terminal ig-like domain (PF02903; HMM-score: 135.7)
    Glyco_hydro_tim (CL0058) GHL6; Hypothetical glycosyl hydrolase 6 (PF14871; HMM-score: 16.3)
    DUF5696; Family of unknown function (DUF5696) (PF18952; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002683
    • TAT(Tat/SPI): 0.000096
    • LIPO(Sec/SPII): 0.000338
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAKITSDALFDHWQVEVSVDFARIQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLPYLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTLDWDLSITPQSDDFFGGDLQGIIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGMKIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFEDYMPKLNTANPEVKDYLLKVATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINCQSMYYKQQISEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKAIFNQSKEDYYLQKEAVALASNCQEIENQLVISPKGFVIFH

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MalR* (repression) regulon, CcpA regulon
    MalR*(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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