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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_1468 [new locus tag: SPH_RS07100 ]
  • pan locus tag?: PNEUPAN002616000
  • symbol: SPH_1468
  • pan gene symbol?: nanB
  • synonym:
  • product: sialidase B (Neuraminidase B)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_1468 [new locus tag: SPH_RS07100 ]
  • symbol: SPH_1468
  • product: sialidase B (Neuraminidase B)
  • replicon: chromosome
  • strand: -
  • coordinates: 1354443..1356665
  • length: 2223
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAAAAAAAATATTAAACAATATGTAACCTTAGGTACTGTAGTGGTATTATCAGCATTT
    GTTGCTAACTCAGTTGCAGCTCAGGAGACTGAAACTTCTGAAGTATCAACACCAAAGTTG
    GTGCAACCTGTTGCACCAACGACTCCGATTTCGGAAGTACAACCTACATCGGATAACTCT
    TCGGAAGTTACTGTACAACCTCGAACAGTTGAAACTACTGTTAAGGATCCATCTTCTACA
    GCGGAAGAAACTCCTGTCTTAGAAAAAAATAATGTTACTTTAACAGGGGGCGGAGAAAAT
    GTTACTAAAGAGTTAAAGGATAAATTTACTAGCGGTGACTTTACTGTAGTGATTAAGTAC
    AATCAGTCAAGTGAGAAAGGCTTACAAGCTCTGTTTGGAATATCTAATTCCAAACCCGGT
    CAACAAAATAGTTATGTAGATGTGTTCCTTAGAGACAATGGTGAGTTGGGGATGGAAGCG
    CGTGATACTTCTTCCAATAAAAATAACCTAGTATCCAGACCTGCTTCAGTTTGGGGTAAG
    TACAAACAAGAGGCTGTGACTAACACTGTTGCAGTAGTAGCAGATTCAGTCAAAAAAACA
    TATTCTTTATACGCAAATGGTACAAAAGTAGTAGAAAAGAAAGTGGATAATTTCCTAAAC
    ATCAAGGATATTAAAGGTATTGATTACTATATGCTTGGGGGAGTGAAACGTGCAGGAAAA
    ACGGCGTTTGGTTTTAACGGAACACTAGAAAATATCAAATTCTTTAATAGTGCATTGGAT
    GAAGAAACTGTTAAAAAGATGACAACAAACGCTGTTACTGGACATTTAATTTATACGGCT
    AATGATACAACAGGTTCTAACTATTTCCGTATTCCAGTTCTGTATACTTTTAGCAATGGT
    CGGGTATTTTCAAGCATTGACGCTCGTTACGGTGGAACTCATGATTTCTTGAATAAAATT
    AATATTGCTACAAGTTATAGTGATGATAATGGTAAGACATGGACTAAACCAAAATTAACA
    TTGGCATTCGATGATTTTGCGCCAGTACCATTAGAATGGCCTCGTGAAGTTGGTGGACGT
    GACTTACAAATCAGCGGTGGTGCAACCTATATTGACTCTGTTATTGTTGAAAAAAAGAAC
    AAACAAGTACTCATGTTTGCTGATGTGATGCCTGCTGGAGTAAGTTTTAGAGAAGCAACT
    AGAAAAGATTCAGGTTATAAACAAATTGATGGTAATTATTACCTTAAATTAAGGAAACAA
    GGTGATACTGATTACAATTATACTATTCGTGAGAATGGTACTGTATACGACGATCGTACC
    AACAGACCAACTGAATTTTCAGTAGATAAAAATTTCGGTATTAAACAAAATGGTAATTAT
    TTGACGGTAGAGCAATATTCGGTTTCATTTGAAAATAATAAAAAGACTGAATATCGTAAT
    GGGACTAAAGTCCATATGAATATCTTCTACAAAGATGCATTGTTTAAAGTAGTGCCAACG
    AACTATATTGCATATATTTCTAGCAATGATCATGGCGAATCTTGGTCTGCACCAACTTTA
    TTACCTCCTATAATGGGACTTAATCGGAATGCGCCATATTTAGGTCCTGGACGTGGAATC
    ATTGAAAGCTCAACTGGACGTATTCTTATTCCGTCTTACACTGGTAAAGAGTCTGCGTTC
    ATTTATAGTGACGATAATGGAGCATCTTGGAAAGTTAAAGTAGTGCCACTTCCTTCTAGT
    TGGTCAGCAGAAGCACAATTTGTAGAATTGAGTCCAGGAGTAATTCAAGCATATATGCGT
    ACAAATAATGGTAAAATTGCATATTTAACAAGTAAAGACGCAGGTACTACTTGGAGTGCA
    CCGGAATATTTGAAATTTGTTTCAAATCCAAGTTATGGAACACAATTATCAATCATCAAT
    TATAGCCAATTGATTGATGGTAAAAAGGCTGTCATTTTAAGTACTCCAAACTCCACAAAT
    GGTCGTAAACACGGACAAATTTGGATTGGTCTAATTAATGATGATAATACAATTGATTGG
    CGTTATCATCACGACGTTGATTATAGTAACTATGGATACTCATATTCAACATTGACAGAG
    TTACCAAATCATGAAATTGGATTGATGTTTGAAAAATTTGATTCATGGTCTCGTAATGAA
    CTTCATATGAAAAATGTTGTACCATATATAACATTTAAGATTGAAGATCTGAAAAAGAAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2223

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_1468 [new locus tag: SPH_RS07100 ]
  • symbol: SPH_1468
  • description: sialidase B (Neuraminidase B)
  • length: 740
  • theoretical pI: 8.59393
  • theoretical MW: 82436.8
  • GRAVY: -0.498378

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.18?  ExPASy
    Exo-alpha-sialidase Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
  • TIGRFAM:
  • TheSEED  :
    • Sialidase (EC 3.2.1.18)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Sialidase (EC 3.2.1.18)
    and 1 more
    Sulfur Metabolism Sulfur Metabolism - no subcategory Galactosylceramide and Sulfatide metabolism  Sialidase (EC 3.2.1.18)
  • PFAM:
    Concanavalin (CL0004) Sialidase; Sialidase, N-terminal domain (PF02973; HMM-score: 126.1)
    and 3 more
    Sialidase (CL0434) BNR_2; BNR repeat-like domain (PF13088; HMM-score: 99.3)
    BNR; BNR/Asp-box repeat (PF02012; HMM-score: 32.3)
    Concanavalin (CL0004) Laminin_G_3; Concanavalin A-like lectin/glucanases superfamily (PF13385; HMM-score: 19.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 1.81
    • Extracellular Score: 8.19
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0008
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0262
    • Extracellular Score: 0.9726
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.980963
    • TAT(Tat/SPI): 0.001497
    • LIPO(Sec/SPII): 0.015522
    • Cleavage Site: CS pos: 27-28. VAA-QE. Pr: 0.8149
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPVAPTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVTKELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEARDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNFLNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHLIYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTWTKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPAGVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDKNFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISSNDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDNGASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYLKFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRYHHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: RpiR* (repression) regulon, NanR* (repression) regulon, CcpA regulon
    RpiR*(TF)important in Sialic acid utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    NanR*(TF)important in Sialic acid utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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